FlyBase Allele Report: Dmel\awd[MSM95]

General Information

Symbol

Dmel\awd^MSM95

Species

D. melanogaster

Name

FlyBase ID

FBal0123032

Feature type

allele

Associated gene

Dmel\awd

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Krishnan et al., 2001)

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

paralytic | heat sensitive (with awd^KRS6)

(Krishnan et al., 2001)

Phenotype Manifest In

optic cartridge | heat sensitive (with awd^KRS6)

(Krishnan et al., 2001)

photoreceptor neuron | heat sensitive (with awd^KRS6)

(Krishnan et al., 2001)

Detailed Description

Statement

Reference

100% of homozygotes are paralysed in 3 minutes at 40^oC.

(Rikhy et al., 2003)

awd^MSM95/awd^KRS6 flies are paralysed at 38^oC. These flies lose the on- and off-transients normally seen in an electroretinogram at 39^oC in a use dependent manner. At 39^oC, membrane invaginations are seen at a low level in the photoreceptor terminals in the lamina in awd^MSM95/awd^KRS6 flies.

(Krishnan et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

awd^MSM95/awd^KRS6 has paralytic | heat sensitive phenotype, suppressible by Hsap\NME1^awd.Tag:HA

(Krishnan et al., 2001)

awd^MSM95/awd^KRS6 has paralytic | heat sensitive phenotype, suppressible by Hsap\NME2^awd.Tag:HA

(Krishnan et al., 2001)

Enhancer of

Statement

Reference

awd^MSM95 is an enhancer of paralytic | heat sensitive phenotype of shi^CK2

(Rikhy et al., 2003, Krishnan et al., 2001)

awd^MSM95 is an enhancer of paralytic | heat sensitive phenotype of sesB^org

(Rikhy et al., 2003)

awd^MSM95/awd^CM47 is an enhancer of paralytic | heat sensitive phenotype of shi^SHY

(Krishnan et al., 2001)

awd^MSM95/awd^MSF15 is an enhancer of paralytic | heat sensitive phenotype of shi^SHY

(Krishnan et al., 2001)

awd^MSM95 is an enhancer of paralytic | temperature conditional phenotype of shi²

(Krishnan et al., 2001)

awd^MSM95 is an enhancer of paralytic | heat sensitive phenotype of shi^SHY

(Krishnan et al., 2001)

awd^MSM95/awd^KRS6 is an enhancer of paralytic | heat sensitive phenotype of shi^CK2

(Krishnan et al., 2001)

NOT Enhancer of

Statement

Reference

awd^MSM95 is a non-enhancer of paralytic | temperature conditional phenotype of para^ts1

(Krishnan et al., 2001)

awd^MSM95 is a non-enhancer of paralytic | heat sensitive phenotype of comt⁶

(Krishnan et al., 2001)

Other

Statement

Reference

awd^MSM95, pn¹ has viable phenotype

(Krishnan et al., 2001)

awd^K/awd^MSM95, pn¹ has lethal phenotype

(Krishnan et al., 2001)

Phenotype Manifest In

Suppressed by

Statement

Reference

awd^MSM95/awd^KRS6 has | heat sensitive phenotype, suppressible | partially by Hsap\NME1^awd.Tag:HA

(Krishnan et al., 2001)

awd^MSM95/awd^KRS6 has | heat sensitive phenotype, suppressible | partially by Hsap\NME2^awd.Tag:HA

(Krishnan et al., 2001)

awd^MSM95 has | heat sensitive phenotype, suppressible by Hsap\NME2^awd.Tag:HA

(Krishnan et al., 2001)

awd^MSM95 has | heat sensitive phenotype, suppressible by Hsap\NME1^awd.Tag:HA

(Krishnan et al., 2001)

Enhancer of

Statement

Reference

awd^MSM95/awd^KRS6 is an enhancer of optic cartridge phenotype of shi^CK2

(Krishnan et al., 2001)

awd^MSM95/awd^KRS6 is an enhancer of photoreceptor neuron phenotype of shi^CK2

(Krishnan et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

100% of sesB^org ; awd^MSM95/awd^MSM95 flies are paralysed in 3 minutes at 34^oC. 100% of shi^CK2 ; awd^MSM95/awd^MSM95 flies are paralysed in 3 minutes at 29^oC. 100% of sesB^org shi^CK2 ; awd^MSM95/awd^MSM95 flies are paralysed in 3 minutes at 27^oC. The on- and off-transients of the electroretinogram (ERG) are lost at 27^oC in sesB^org shi^CK2/Y ; awd^MSM95/awd^MSM95 flies.

(Rikhy et al., 2003)

The temperature at which 100% of shi², shi^SHY or shi^CK2 flies are paralysed is reduced if they also carry awd^MSM95. The temperature at which the ERG on- and off-transients are lost is lowered to 30^oC in shi^CK2 flies that also carry awd^MSM95/awd^KRS6. The temperature at which the membrane invaginations are seen in the photoreceptor terminals in the lamina in shi^CK2 flies is reduced if they are also carrying awd^MSM95/awd^KRS6. shi^CK2/Y ; awd^MSM95/awd^KRS6 motor terminals show reduced uptake of an endocytosis tracer at 38^oC compared to controls. The general morphology of the motor nerve endings appears normal in these flies.