FB2024_03 , released June 25, 2024
Allele: Dmel\shiSHY
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General Information
Symbol
Dmel\shiSHY
Species
D. melanogaster
Name
FlyBase ID
FBal0065690
Feature type
allele
Associated gene
Dmel\shi
Associated Insertion(s)
Carried in Construct
Also Known As
Sushi
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Ramaswami et al., 1993)
Progenitor genotype
shi2
(Narayanan et al., 2005)
Cytology
Description

Amino acid replacement: T749I.

(Narayanan et al., 2005)

The T749I substitution maps to the COOH-terminal region of the GTPase effector domain of the shi protein.

(Narayanan et al., 2005)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
X:15,901,996..15,901,996 [+]
Nucleotide change:

C15901996T

Amino acid change:

T740I | shi-PA; T740I | shi-PB; T740I | shi-PC; T740I | shi-PE; T740I | shi-PF; T740I | shi-PG; T740I | shi-PH; T740I | shi-PI; T740I | shi-PJ; T740I | shi-PK; T746I | shi-PL; T746I | shi-PM; T759I | shi-PN; T744I | shi-PO; T744I | shi-PP

Reported amino acid change:

T749I

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Narayanan et al., 2005)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Mutant flies are paralysed at 34.5oC.

      (Krishnan et al., 2001)

      Flies are not paralytic at 28oC. Mutation also suppresses the paralytic phenotype of shi1, the eye, thoracic bristle, wing and wing margin bristle phenotypes.

      (Ramaswami et al., 1993)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Statement
      Reference

      shiSHY has paralytic | heat sensitive phenotype, enhanceable by sesBorg

      (Rikhy et al., 2003)

      shiSHY has paralytic | heat sensitive phenotype, enhanceable by awdCM47

      (Krishnan et al., 2001)

      shiSHY has paralytic | heat sensitive phenotype, enhanceable by awdCM47/awdMSF15

      (Krishnan et al., 2001)

      shiSHY has paralytic | heat sensitive phenotype, enhanceable by awdMSF15

      (Krishnan et al., 2001)

      shiSHY has paralytic | heat sensitive phenotype, enhanceable by awdMSM95/awdCM47

      (Krishnan et al., 2001)

      shiSHY has paralytic | heat sensitive phenotype, enhanceable by awdMSM95/awdMSF15

      (Krishnan et al., 2001)

      shiSHY has paralytic | heat sensitive phenotype, enhanceable by awdMSM95

      (Krishnan et al., 2001)
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      sesBorg lowers the temperature at which 100% of shiSHY flies are paralysed in 3 minutes, from 35oC to 33oC.

      (Rikhy et al., 2003)

      The temperature at which 100% of shiSHY flies are paralysed is reduced if they also carry awdMSM95, awdCM47 or awdMSF15.

      (Krishnan et al., 2001)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Complements

      shi2

      (Narayanan et al., 2005)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer

      Revertant: complete

      (Narayanan et al., 2005)

      The T749I substitution acts as a intragenic suppressor of the paralytic phenotype and synaptic defects of shi2.

      (Narayanan et al., 2005)

      Selected as: suppressor of shi2.

      (Narayanan et al., 2005)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (6)
      Reported As
      Symbol Synonym
      Shy
      (Narayanan et al., 2005)
      Sushi
      (Narayanan et al., 2005, Ramaswami et al., 1993)
      shiSHY
      shiShy
      (Krishnan et al., 1996)
      shishy
      (Narayanan et al., 2005)
      Name Synonyms
      Suppressor of shi
      (Narayanan et al., 2005)
      Secondary FlyBase IDs
        References (5)