FlyBase Allele Report: Dmel\pn[1]

General Information

Symbol

Dmel\pn¹

Species

D. melanogaster

Name

FlyBase ID

FBal0013871

Feature type

allele

Associated gene

Dmel\pn

Associated Insertion(s)

422{}pn¹

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - molecular evidence

Mutagen

spontaneous

Nature of the Allele

Allele class

hypomorphic allele - molecular evidence

(Chen et al., 2019)

Mutagen

spontaneous

(Teng et al., 1991)

Progenitor genotype

Associated Insertion(s)

422{}pn¹

Cytology

Description

422 element insertion.

(Timmons and Shearn, 1996)

Insertion of the 422 element into the pn locus.

(Frolov et al., 1994)

Insert stated as cause: undefined

(Teng et al., 1991)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

X:2,181,352..2,182,939

(Timmons and Shearn, 1996, Frolov et al., 1994)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal circadian behavior | adult stage

(Chen et al., 2019)

abnormal eye color

(Chen et al., 2019, Timmons and Shearn, 1996, Teng et al., 1991)

abnormal locomotor rhythm | adult stage

(Chen et al., 2019)

abnormal oxidative stress response | adult stage

(Chen et al., 2019)

increased mortality | adult stage | chemical conditional

(Chen et al., 2019)

visible | recessive

Phenotype Manifest In

embryonic/larval proventriculus | third instar larval stage

(Chen et al., 2019)

mitochondrion | third instar larval stage

(Chen et al., 2019)

pigment cell

(Timmons and Shearn, 1996, Teng et al., 1991)

Detailed Description

Statement

Reference

pn¹ third instar larvae exhibit a significant reduction in the percentage of cellular area marked for mitochondria (sqh-mitoEYFP) in the proventriculus cells from the foregut compared to controls. pn¹ adults also exhibit brownish-purple eyes and an increased sensitivity to oxidative stress (induced by feeding with paraquat) when compared to controls.

pn¹ adults exhibit a significant reduction in the amplitude of locomotor activity rhythm (circadian rhythm) under constant darkness while they exhibit normal circadian periods with normal percentages of rhythmic flies under constant darkness; normal bimodal activity patterns and anticipation under light-dark condition; normal cell bodies and axonal projections of PDF-expressing circadian pacemaker neurons when compared to controls.

(Chen et al., 2019)

Suppresses hop^Tum lethality to same extent as awd^K.

(Zinyk et al., 1993)

Eye colour: brownish-red.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

awd^MSM95, pn¹ has viable phenotype

(Krishnan et al., 2001)

awd^K/awd^KRS6, pn¹ has lethal phenotype

(Krishnan et al., 2001)

awd^K/awd^MSM95, pn¹ has lethal phenotype

(Krishnan et al., 2001)

awd^K, pn¹ has lethal phenotype

(Krishnan et al., 2001, Timmons and Shearn, 1996)

awd^K, pn¹ has lethal | second instar larval stage phenotype

(Sturtevant, 1955)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

pn¹ has phenotype, non-suppressible by su(Hw)²

(Lindsley and Grell, 1968)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

pn¹ is rescued by pn^hs.3

(Timmons and Shearn, 1996)

Partially rescued by

pn¹ is partially rescued by pn^hs.PZ

(Chen et al., 2019)

Not rescued by

pn¹ is not rescued by pn^hs.4

(Timmons and Shearn, 1996)

Comments

pn^hs.4 fails to rescue the lethal interaction of pn¹ with awd^K. pn^hs.3 does rescue the lethality (with or without heat shock) and male progeny are recovered. Eye colour of P{HS-PN⁺4} transformants remains pn even after several heat pulses, P{HS-PN⁺3} transformants (without heat shock or with one or numerous heat shocks) exhibit wild-type colouration.