FlyBase Allele Report: Dmel\awd[KRS6]

General Information

Symbol

Dmel\awd^KRS6

Species

D. melanogaster

Name

FlyBase ID

FBal0030537

Feature type

allele

Associated gene

Dmel\awd

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Xu et al., 1996, Timmons et al., 1993)

Mutagen

ethyl methanesulfonate

(Timmons et al., 1993)

Progenitor genotype

awd^K

(Timmons et al., 1993)

Cytology

Description

788bp deletion that removes the entire protein coding region.

(Timmons et al., 1995)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of pupal stage | recessive

(Xu et al., 1996)

paralytic | heat sensitive (with awd^MSM95)

(Krishnan et al., 2001)

some die during pupal stage | recessive

(Xu et al., 1996)

Phenotype Manifest In

egg chamber

(Woolworth et al., 2009)

optic cartridge | heat sensitive (with awd^MSM95)

(Krishnan et al., 2001)

photoreceptor neuron | heat sensitive (with awd^MSM95)

(Krishnan et al., 2001)

Detailed Description

Statement

Reference

awd^KRS6 females in which the lethality has been rescued by expression of awd^hs.P at 29[o]C and then the females have been shifted to 18[o]C for 3 days have very few intact egg chambers. The few surviving egg chambers are reduced and uneven and show disrupted epithelial organisation and epithelial discontinuity.

(Woolworth et al., 2009)

awd^MSM95/awd^KRS6 flies are paralysed at 38^oC. These flies lose the on- and off-transients normally seen in an electroretinogram at 39^oC in a use dependent manner. At 39^oC, membrane invaginations are seen at a low level in the photoreceptor terminals in the lamina in awd^MSM95/awd^KRS6 flies.

(Krishnan et al., 2001)

Ovaries from homozygous larvae transplanted into female sterile host larvae fail to develop egg chambers. 21 of 26 egg laying females generated from embryos that had been transplanted with awd^KRS6 pole cells gave rise to viable progeny, the females are heterozygous. The remaining 5 females are homozygous and give rise to embryos that survive as late as the blastoderm stage. Surprisingly females carrying germline clones give rise to viable progeny, the only source of awd present is in the cytoplasm of the heterozygous clone mother cells.

(Xu et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

awd^MSM95/awd^KRS6 has paralytic | heat sensitive phenotype, suppressible by Hsap\NME1^awd.Tag:HA

(Krishnan et al., 2001)

awd^MSM95/awd^KRS6 has paralytic | heat sensitive phenotype, suppressible by Hsap\NME2^awd.Tag:HA

(Krishnan et al., 2001)

Enhancer of

Statement

Reference

awd^MSM95/awd^KRS6 is an enhancer of paralytic | heat sensitive phenotype of shi^CK2

(Krishnan et al., 2001)

Other

Statement

Reference

awd^K/awd^KRS6, pn¹ has lethal phenotype

(Krishnan et al., 2001)

Phenotype Manifest In

Suppressed by

Statement

Reference

awd^MSM95/awd^KRS6 has | heat sensitive phenotype, suppressible | partially by Hsap\NME1^awd.Tag:HA

(Krishnan et al., 2001)

awd^MSM95/awd^KRS6 has | heat sensitive phenotype, suppressible | partially by Hsap\NME2^awd.Tag:HA

(Krishnan et al., 2001)

awd^KRS6 has phenotype, suppressible by Hsap\NME1^awd.Tag:HA

(Xu et al., 1996)

awd^KRS6 has phenotype, suppressible by Hsap\NME2^awd.Tag:HA

(Xu et al., 1996)

Enhancer of

Statement

Reference

awd^MSM95/awd^KRS6 is an enhancer of optic cartridge phenotype of shi^CK2

(Krishnan et al., 2001)

awd^MSM95/awd^KRS6 is an enhancer of photoreceptor neuron phenotype of shi^CK2

(Krishnan et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

The temperature at which the ERG on- and off-transients are lost is lowered to 30^oC in shi^CK2 flies that also carry awd^MSM95/awd^KRS6. The temperature at which the membrane invaginations are seen in the photoreceptor terminals in the lamina in shi^CK2 flies is reduced if they are also carrying awd^MSM95/awd^KRS6. shi^CK2/Y ; awd^MSM95/awd^KRS6 motor terminals show reduced uptake of an endocytosis tracer at 38^oC compared to controls. The general morphology of the motor nerve endings appears normal in these flies.

(Krishnan et al., 2001)

Hsap\NME1^{awd.T:Ivir\HA1} exhibits partial rescue of the awd^KRS6 mutant zygotic phenotype, a greater degree of rescue is seen with Hsap\NME2^{awd.T:Ivir\HA1}.

(Xu et al., 1996)

Xenogenetic Interactions

Statement

Reference

Hsap\NME1^{awd.T:Ivir\HA1} exhibits partial rescue of the awd^KRS6 mutant zygotic phenotype, a greater degree of rescue is seen with Hsap\NME2^{awd.T:Ivir\HA1}.

(Xu et al., 1996)

Complementation and Rescue Data

Fails to complement

awd^MSM95

(Krishnan et al., 2001)

Rescued by

awd^KRS6 is rescued by awd^hs.P

(Woolworth et al., 2009)

awd^MSM95/awd^KRS6 is rescued by awd^+mPAS7

(Krishnan et al., 2001)

Not rescued by

awd^MSM95/awd^KRS6 is not rescued by awd^H119A