FB2024_03 , released June 25, 2024
Allele: Dmel\shiCK2
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General Information
Symbol
Dmel\shiCK2
Species
D. melanogaster
Name
FlyBase ID
FBal0082267
Feature type
allele
Associated gene
Dmel\shi
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Mutagen
    Nature of the Allele
    Allele class
    Mutagen
    Progenitor genotype
    Cytology
    Description
    Mutations Mapped to the Genome
    Curation Data
    Type
    Location
    Additional Notes
    References
    Variant Molecular Consequences
    Associated Sequence Data
    DDBJ / EMBL / GenBank
    DNA sequence
    Protein sequence
     
    UniProtKB/Swiss-Prot
      UniProtKB/TrEMBL
        Expression Data
        Reporter Expression
        Additional Information
        Statement
        Reference
         
        Marker for
        Reflects expression of
        Reporter construct used in assay
        Human Disease Associations
        Disease Ontology (DO) Annotations
        Models Based on Experimental Evidence ( 0 )
        Disease
        Evidence
        References
        Modifiers Based on Experimental Evidence ( 0 )
        Disease
        Interaction
        References
        Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
         
        Disease-implicated variant(s)
         
        Phenotypic Data
        Phenotypic Class
        Phenotype Manifest In
        Detailed Description
        Statement
        Reference

        100% of shiCK2 flies completely paralyse within about 2 minutes at 35oC. At 33oC, no shiCK2 flies are paralysed within 3 minutes. The on- and off-transients of the electroretinogram (ERG) are lost at 36oC in shiCK2/Y flies.

        (Rikhy et al., 2003)

        Mutant flies are paralysed at 35oC. At 36oC the on- and off-transients normally seen in an electroretinogram are lost. At 31oC shiCK2 flies show characteristic recycling intermediates (deep membrane invaginations) in the photoreceptor terminals in the lamina.

        (Krishnan et al., 2001)

        Sub-anaesthetic concentration of carbon dioxide specifically suppresses the temperature sensitive paralytic phenotype.

        (Krishnan et al., 1996)
        External Data
        Interactions
        Show genetic interaction network for Enhancers & Suppressors
        Phenotypic Class
        Enhanced by
        Statement
        Reference

        shiCK2 has abnormal neurophysiology | heat sensitive phenotype, enhanceable by sesBorg

        (Rikhy et al., 2003)

        shiCK2 has paralytic | heat sensitive phenotype, enhanceable by awdMSM95

        (Rikhy et al., 2003, Krishnan et al., 2001)

        shiCK2 has paralytic | heat sensitive phenotype, enhanceable by sesBorg/sesBorg

        (Rikhy et al., 2003)

        shiCK2 has paralytic | heat sensitive phenotype, enhanceable by awdCM47

        (Krishnan et al., 2001)

        shiCK2 has paralytic | heat sensitive phenotype, enhanceable by awdMSF15

        (Krishnan et al., 2001)

        shiCK2 has paralytic | heat sensitive phenotype, enhanceable by awdMSM95/awdKRS6

        (Krishnan et al., 2001)
        NOT Enhanced by
        Statement
        Reference

        shiCK2 has paralytic | heat sensitive phenotype, non-enhanceable by awdK/awdK

        (Krishnan et al., 2001)
        Phenotype Manifest In
        Enhanced by
        Statement
        Reference

        shiCK2 has optic cartridge phenotype, enhanceable by awdMSM95/awdKRS6

        (Krishnan et al., 2001)

        shiCK2 has photoreceptor neuron phenotype, enhanceable by awdMSM95/awdKRS6

        (Krishnan et al., 2001)
        Additional Comments
        Genetic Interactions
        Statement
        Reference

        sesBorg lowers the temperature at which 100% of shiCK2 flies are paralysed in 3 minutes, from 35oC to 33oC. 100% of shiCK2 ; awdMSM95/awdMSM95 flies are paralysed in 3 minutes at 29oC. 100% of sesBorg shiCK2 ; awdMSM95/awdMSM95 flies are paralysed in 3 minutes at 27oC. The on- and off-transients of the electroretinogram (ERG) are lost at 33oC in sesBorg shiCK2/Y flies. The on- and off-transients of the electroretinogram (ERG) are lost at 27oC in sesBorg shiCK2/Y ; awdMSM95/awdMSM95 flies.

        (Rikhy et al., 2003)

        The temperature at which 100% of shiCK2 flies are paralysed is reduced if they also carry awdMSM95, awdCM47 or awdMSF15. The temperature at which the ERG on- and off-transients are lost is lowered to 30oC in shiCK2 flies that also carry awdMSM95/awdKRS6. The temperature at which the membrane invaginations are seen in the photoreceptor terminals in the lamina in shiCK2 flies is reduced if they are also carrying awdMSM95/awdKRS6. shiCK2/Y ; awdMSM95/awdKRS6 motor terminals show reduced uptake of an endocytosis tracer at 38oC compared to controls. The general morphology of the motor nerve endings appears normal in these flies.

        (Krishnan et al., 2001)
        Xenogenetic Interactions
        Statement
        Reference
        Complementation and Rescue Data
        Comments
        Images (0)
        Mutant
        Wild-type
        Stocks (0)
        Notes on Origin
        Discoverer
        External Crossreferences and Linkouts ( 0 )
        Synonyms and Secondary IDs (1)
        Reported As
        Symbol Synonym
        shiCK2
        (Rikhy et al., 2007)
        Name Synonyms
        Secondary FlyBase IDs
          References (5)