FlyBase Allele Report: Dmel\mam[H.UAS]

General Information

Symbol

Dmel\mam^H.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0102514

Feature type

allele

Associated gene

Dmel\mam

Associated Insertion(s)

Carried in Construct

P{UAS-mamH}

Also Known As

UAS-MamH, MamH

Key Links

Transgenic product class

inframe_deletion

(Helms et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Helms et al., 1999)

Mutagen

in vitro construct

(Helms et al., 1999)

Progenitor genotype

Carried in construct

P{UAS-mamH}

Cytology

Description

A truncated mam open reading frame, ending at nucleotide 1489 (amino acid residue 245), is driven under the control of UASt sequences. Sequences located carboxy to the basic cluster are removed, including both acidic charge clusters.

(Helms et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

mam

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | heat sensitive, with Scer\GAL4^hs.PH

(Yedvobnick et al., 2004)

visible

(Helms et al., 1999)

visible, with Scer\GAL4³⁰⁹

(Helms et al., 1999)

visible, with Scer\GAL4^bbg-C96

(Kwon et al., 2013, Yedvobnick et al., 2001)

visible | adult stage, with Scer\GAL4³⁰⁹

(Alexander et al., 2006)

visible | adult stage, with Scer\GAL4³⁰⁹, mam^EP2022.A

(Alexander et al., 2006)

visible | adult stage, with Scer\GAL4^bbg-C96

(Alexander et al., 2006)

visible | adult stage, with Scer\GAL4^bbg-C96, mam^EP2022.A

(Alexander et al., 2006)

visible | heat sensitive, with Scer\GAL4^hs.PH

(Yedvobnick et al., 2004)

Phenotype Manifest In

eye | heat sensitive, with Scer\GAL4^hs.PH

(Yedvobnick et al., 2004)

female germline stem cell, with Scer\GAL4^C587

(Song et al., 2007)

germarium cap cell, with Scer\GAL4^C587

(Song et al., 2007)

larval RP2 motor neuron | ectopic | heat sensitive, with Scer\GAL4^hs.PH

(Yedvobnick et al., 2004)

macrochaeta, with Scer\GAL4³⁰⁹

(Helms et al., 1999)

macrochaeta | ectopic, with Scer\GAL4³⁰⁹

(Helms et al., 1999)

macrochaeta | increased number | heat sensitive, with Scer\GAL4^hs.PH

(Yedvobnick et al., 2004)

notopleural bristle, with Scer\GAL4³⁰⁹

(Helms et al., 1999)

posterior notopleural bristle, with Scer\GAL4³⁰⁹

(Alexander et al., 2006)

posterior notopleural bristle, with Scer\GAL4³⁰⁹, mam^EP2022.A

(Alexander et al., 2006)

posterior notopleural bristle | ectopic, with Scer\GAL4³⁰⁹

(Helms et al., 1999)

RP2sib neuron | heat sensitive, with Scer\GAL4^hs.PH

(Yedvobnick et al., 2004)

scutellar bristle

(Helms et al., 1999)

scutellar bristle, with Scer\GAL4³⁰⁹

(Helms et al., 1999)

scutellar bristle | ectopic, with Scer\GAL4³⁰⁹

(Helms et al., 1999)

wing, with Scer\GAL4^bbg-C96

(Yedvobnick et al., 2001, Helms et al., 1999)

wing | heat sensitive, with Scer\GAL4^hs.PH

(Yedvobnick et al., 2004)

wing margin, with Scer\GAL4^bbg-C96

(Kwon et al., 2013, Alexander et al., 2006)

wing margin, with Scer\GAL4^bbg-C96, mam^EP2022.A

(Alexander et al., 2006)

wing margin bristle, with Scer\GAL4^bbg-C96

(Helms et al., 1999)

Detailed Description

Statement

Reference

Expression of mam^H.Scer\UAS under the control of Scer\GAL4^bbg-C96 results in a 100% penetrant wing nicking phenotype.

(Kwon et al., 2013)

The number of cap cells and germline stem cells in the ovaries of 2 day old females expressing mam^H.Scer\UAS under the control of Scer\GAL4^C587 is significantly reduced compared to wild type.

(Song et al., 2007)

Expression of mam^H.Scer\UAS under the control of Scer\GAL4^C96 produces nicks around the wing margin with 100% penetrance (whereas mam^H.Scer\UAS heterozygotes do not exhibit any wing phenotypes). This phenotype is partially suppressed in flies also heterozygous for mam^EP2022.A.

Expression of mam^H.Scer\UAS under the control of Scer\GAL4³⁰⁹ elicits an increase in the number of bristles at the site of the posterior notopleural macrochaete (whereas mam^H.Scer\UAS heterozygotes do not exhibit any bristle phenotypes at this site). This phenotype is partially suppressed in flies also heterozygous for mam^EP2022.A.

(Alexander et al., 2006)

mam^H.Scer\UAS; Scer\GAL4¹¹⁵¹ pupae have normal numbers of dorso-ventral indirect flight muscle founder cells.

(Dutta et al., 2004)

Expression of mam^H.Scer\UAS under the control of Scer\GAL4^hs.PH between 2 and 6 hours of development results in a neurogenic phenotype. Expression of mam^H.Scer\UAS under the control of Scer\GAL4^hs.PH between 6 and 8 hours of development results in the GMC-1 dividing symmetrically into two RP2 neurons in about 10% of hemisegments. Expression of mam^H.Scer\UAS under the control of Scer\GAL4^hs.PH during the third larval instar results in duplications of the macrochaetae, scarring of the eye and severe loss of the wing blade.

(Yedvobnick et al., 2004)

Shows a low level of scutellar bristle duplications even without a Scer\GAL4 driver. When driven by Scer\GAL4³⁰⁹ perturbations are observed in bristle number predominantly at scutellar and notopleural sites, duplications of macrochaetae are seen at most scutellar sites and posterior notopleural sites. The macrochaetae multiplication phenotype is enhanced by the addition of mam¹. When mam^H.Scer\UAS is driven by Scer\GAL4^C96 wing nicks are seen and there is a loss of wing margin bristles. The addition of mam¹ produces a stronger wing nicking phenotype that is incompletely penetrant.

(Helms et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^bbg-C96, mam^H.UAS has visible phenotype, enhanceable by lola^EP-1000

(Kwon et al., 2013)

Scer\GAL4^bbg-C96, mam^H.UAS has visible phenotype, enhanceable by lola^EP-1538

(Kwon et al., 2013)

Scer\GAL4^bbg-C96, mam^H.UAS has visible phenotype, enhanceable by pum^EP-1646

(Kwon et al., 2013)

Scer\GAL4^bbg-C96, mam^H.UAS has visible phenotype, enhanceable by lilli^EP-1630/Scer\GAL4^bbg-C96

(Kwon et al., 2013)

Scer\GAL4^bbg-C96, mam^H.UAS has visible phenotype, enhanceable by Lk6²

(Kwon et al., 2013)

Scer\GAL4^bbg-C96, mam^H.UAS has visible phenotype, enhanceable by Pabp2^EP-558

(Kwon et al., 2013)

Scer\GAL4^bbg-C96, mam^H.UAS has visible phenotype, enhanceable by EcR^EP-939

(Kwon et al., 2013)

Scer\GAL4^bbg-C96, mam^H.UAS has visible | adult stage phenotype, enhanceable by RpL13A^EP3614.A, Scer\GAL4^bbg-C96

(Alexander et al., 2006)

Scer\GAL4^bbg-C96, mam^H.UAS has visible | adult stage phenotype, enhanceable by Delta^EP3816.A, Scer\GAL4^bbg-C96

(Alexander et al., 2006)

Scer\GAL4³⁰⁹, mam^H.UAS has visible | adult stage phenotype, enhanceable by Delta^EP3816.A, Scer\GAL4³⁰⁹

(Alexander et al., 2006)

NOT Enhanced by

Statement

Reference

Scer\GAL4^bbg-C96, mam^H.UAS has visible phenotype, non-enhanceable by Scer\GAL4^bbg-C96/Tudor-SN^EP-593

(Kwon et al., 2013)

Suppressed by

Statement

Reference

Scer\GAL4^bbg-C96, mam^H.UAS has visible phenotype, suppressible by tara^EP-425

(Kwon et al., 2013)

Scer\GAL4^bbg-C96, mam^H.UAS has visible phenotype, suppressible by Lk6^EP-573

(Kwon et al., 2013)

Scer\GAL4^bbg-C96, mam^H.UAS has visible phenotype, suppressible by Atg1^EP-1202

(Kwon et al., 2013)

Scer\GAL4^bbg-C96, mam^H.UAS has visible phenotype, suppressible by wdb^EP-1037/Scer\GAL4^bbg-C96

(Kwon et al., 2013)

Scer\GAL4^bbg-C96, mam^H.UAS has visible phenotype, suppressible by Scer\GAL4^bbg-C96/emc^EP-1561

(Kwon et al., 2013)

Scer\GAL4^bbg-C96, mam^H.UAS has visible phenotype, suppressible by emc^D

(Kwon et al., 2013)

Scer\GAL4³⁰⁹, mam^H.UAS has visible | adult stage phenotype, suppressible by RpL13A^EP3614.A, Scer\GAL4³⁰⁹

(Alexander et al., 2006)

NOT suppressed by

Statement

Reference

Scer\GAL4^bbg-C96, mam^H.UAS has visible phenotype, non-suppressible by Scer\GAL4^bbg-C96/Tudor-SN^EP-593

(Kwon et al., 2013)

Enhancer of

Statement

Reference

mam^H.UAS, Scer\GAL4^bbg-C96 is an enhancer of visible phenotype of Rbf^280.UAS, Scer\GAL4^bbg-C96

(Kwon et al., 2013)

Other

Statement

Reference

Df(1)N-54l9, Scer\GAL4³⁰⁹, mam^H.UAS has lethal phenotype

(Helms et al., 1999)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^bbg-C96, mam^H.UAS has wing margin phenotype, enhanceable by lola^EP-1000

(Kwon et al., 2013)

Scer\GAL4^bbg-C96, mam^H.UAS has wing margin phenotype, enhanceable by lola^EP-1538

(Kwon et al., 2013)

Scer\GAL4^bbg-C96, mam^H.UAS has wing margin phenotype, enhanceable by pum^EP-1646

(Kwon et al., 2013)

Scer\GAL4^bbg-C96, mam^H.UAS has wing margin phenotype, enhanceable by lilli^EP-1630/Scer\GAL4^bbg-C96

(Kwon et al., 2013)

Scer\GAL4^bbg-C96, mam^H.UAS has wing margin phenotype, enhanceable by Lk6²

(Kwon et al., 2013)

Scer\GAL4^bbg-C96, mam^H.UAS has wing margin phenotype, enhanceable by Pabp2^EP-558

(Kwon et al., 2013)

Scer\GAL4^bbg-C96, mam^H.UAS has wing margin phenotype, enhanceable by EcR^EP-939

(Kwon et al., 2013)

Scer\GAL4^bbg-C96, mam^H.UAS has wing margin phenotype, enhanceable by RpL13A^EP3614.A, Scer\GAL4^bbg-C96

(Alexander et al., 2006)

Scer\GAL4^bbg-C96, mam^H.UAS has wing margin phenotype, enhanceable by Delta^EP3816.A, Scer\GAL4^bbg-C96

(Alexander et al., 2006)

Scer\GAL4³⁰⁹, mam^H.UAS has posterior notopleural bristle phenotype, enhanceable by Delta^EP3816.A, Scer\GAL4³⁰⁹

(Alexander et al., 2006)

NOT Enhanced by

Statement

Reference

Scer\GAL4^bbg-C96, mam^H.UAS has wing margin phenotype, non-enhanceable by Scer\GAL4^bbg-C96/Tudor-SN^EP-593

(Kwon et al., 2013)

Suppressed by

Statement

Reference

Scer\GAL4^bbg-C96, mam^H.UAS has wing margin phenotype, suppressible by tara^EP-425

(Kwon et al., 2013)

Scer\GAL4^bbg-C96, mam^H.UAS has wing margin phenotype, suppressible by Lk6^EP-573

(Kwon et al., 2013)

Scer\GAL4^bbg-C96, mam^H.UAS has wing margin phenotype, suppressible by Atg1^EP-1202

(Kwon et al., 2013)

Scer\GAL4^bbg-C96, mam^H.UAS has wing margin phenotype, suppressible by wdb^EP-1037/Scer\GAL4^bbg-C96

(Kwon et al., 2013)

Scer\GAL4^bbg-C96, mam^H.UAS has wing margin phenotype, suppressible by Scer\GAL4^bbg-C96/emc^EP-1561

(Kwon et al., 2013)

Scer\GAL4³⁰⁹, mam^H.UAS has posterior notopleural bristle phenotype, suppressible by RpL13A^EP3614.A, Scer\GAL4³⁰⁹

(Alexander et al., 2006)

NOT suppressed by

Statement

Reference

Scer\GAL4^bbg-C96, mam^H.UAS has wing margin phenotype, non-suppressible by Scer\GAL4^bbg-C96/Tudor-SN^EP-593

(Kwon et al., 2013)

Enhancer of

Statement

Reference

mam^H.UAS, Scer\GAL4^bbg-C96 is an enhancer of wing margin phenotype of Rbf^280.UAS, Scer\GAL4^bbg-C96

(Kwon et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

The wing nicking phenotype caused by expression of mam^H.Scer\UAS under the control of Scer\GAL4^bbg-C96 is suppressed if the flies are also carrying one of tara^EP-425, Lk6^EP-573, emc^D or Atg1^EP-1202 or are co-expressing either wdb^EP-1037 or emc^EP-1561.

The wing nicking phenotype caused by expression of mam^H.Scer\UAS under the control of Scer\GAL4^bbg-C96 is enhanced if the flies are also carrying one of Pabp2^EP-558, EcR^EP-939, lola^EP-1000, lola^EP-1538, Lk6², or pum^EP-1646 or are co-expressing lilli^EP-1630.

Co-expression of Tudor-SN^EP-593 has no effect on the wing nicking phenotype caused by expression of mam^H.Scer\UAS under the control of Scer\GAL4^bbg-C96.

The wing nicking phenotype caused by expression of Rbf^280.Scer\UAS under the control of Scer\GAL4^bbg-C96 is enhanced if the flies are also co-expressing mam^H.Scer\UAS.

(Kwon et al., 2013)

The wing margin nicking phenotype produced by expression of mam^H.Scer\UAS under the control of Scer\GAL4^C96 is enhanced in flies also heterozygous for RpL13A^EP3614.A.

The wing margin nicking phenotype produced by expression of mam^H.Scer\UAS under the control of Scer\GAL4^C96 is slightly enhanced in flies also heterozygous for Dl^EP3816.A, though the phenotypic effects are largely additive.

The increase in bristle number at the site of the posterior notopleural macrochaete produced by expression of mam^H.Scer\UAS under the control of Scer\GAL4³⁰⁹ is enhanced in flies also heterozygous for Dl^EP3816.A.

The increase in bristle number at the site of the posterior notopleural macrochaete produced by expression of mam^H.Scer\UAS under the control of Scer\GAL4³⁰⁹ is slightly suppressed in flies also heterozygous for RpL13A^EP3614.A as fewer bristles are evident in some cases.

(Alexander et al., 2006)

The addition of Df(1)N-54l9 to flies expressing mam^H.Scer\UAS under the control of Scer\GAL4³⁰⁹ produces lethality.

(Helms et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

26673

w^*; P{UAS-mamH}2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

mam^H.Scer\UAS

mam^H.UAS

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

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