In wild-type animals, exposure to 40Gy of γ rays induces a distinct pattern of apoptosis in the wing disc, focussed on the the wing pouch, but excluding the DV boundary region and prospective vein. In Dp^a4/Dp^a2 animals, the same irradiation treatment induces a different pattern of apoptosis, a smaller number of apoptotic cells are clustered in the center of the wing pouch, many in the D-V boundary region or prospective veins. This new pattern of apoptosis is consistent over time and follows the same kinetics as in wild-type. Marker analysis suggests that patterning of the wing disc (including vein and D-V boundary specification) is normal in these animals.

Dp^a2 animals partially rescued by Dp^hs.PD have rough eyes and are female sterile. Nurse cell heterochromatin is also increased. In Dp^a2/Df(2R)vg56 egg chambers, unlike wild-type (where nurse cells undergo apoptosis during stages 12 to 13 and have degenerated by stage 14), degeneration of nurse cells is abnormal and occurs late if at all.

Females carrying homozygous germline clones are sterile. The females can produce some eggs, but at a rate far less than wild-type females. The eggs are all abnormal and do not develop. There is a highly penetrant chorion defect; the dorsal appendages are replaced with a single fused appendage. The fused appendage varies in length and shape (they are often short and wide) and in the degree of fusion; some appendages are fused only at the base, or are fused at the base and at the tips but not in the middle. The eggs are smaller than normal, with some only half normal size. Ovarioles containing homozygous germline clones contain homozygous mutant egg chambers that progress to the final stages of oogenesis and deposit chorion material around the oocyte. These late stage oocytes are much smaller than normal and the chorions are ventralised. Young egg chambers show an aberrant accumulation of actin in several brightly stained foci, which persist throughout oogenesis. Actin bundles do form in mutant nurse cells in late stage egg chambers, but their density is reduced compared to wild type. Mutant nurse cells fail to degenerate on schedule. Few if any stage 13 dumpless egg chambers contain TUNEL-positive apoptotic nuclei (in contrast to wild type). Approximately 26% of mutant egg chambers contain approximately double the normal number of nurse cell nuclei. Single oocytes with five ring canals are seen in some of these egg chambers, suggesting that an additional germline mitosis has occurred.

Hemizygous Dp^a2 adults that are partly rescued from lethality by basal expression of Dp^hs.PD produce thin eggshells. Ovaries show abnormal patterns of BrdU incorporation during stage 10B and later stages. The follicle cell nuclei fail to initiate amplification in most egg chambers, while in some stage 10B egg chambers genomic replication rather than amplification is seen.

Disrupt G[[1]]-S transcription late in embryogenesis, DNA replication still occurs.

Hemizygotes exhibit kidney-shaped rough eyes, thin and short bristles on the thorax and head and severe abdominal abnormalities.

Dp^a1/Dp^a2 flies exhibit reduced eyes, missing and disorganised ommatidia, stunted, missing and disorganised bristles. Daily heat shocks of Dp^hs.PD rescued proliferation defects to normal looking eyes.

Dp^a1/Dp^a2 is a suppressor of abnormal mitotic cell cycle phenotype of Rheb^UAS.cSa, Scer\GAL4^Act5C.PP

Dp^a1/Dp^a2 is a suppressor of abnormal mitotic cell cycle phenotype of Myc^UAS.cZa, Scer\GAL4^Act5C.PP

Df(2R)vg-B/Dp^a2 is a suppressor of abnormal developmental rate phenotype of E2f1⁹¹/E2f1^rM729

CycD², Dp^a2 has partially lethal | dominant phenotype

CycD¹, Dp^a2 has partially lethal | dominant phenotype

Dp^a1/Dp^a2, Scer\GAL4^Act5C.PP, dap^UAS.cdNa has abnormal mitotic cell cycle phenotype

Dp^a1/Dp^a2, Scer\GAL4^Act5C.PP, Wee1^UAS.cPa has abnormal mitotic cell cycle phenotype

Dp^a1/Dp^a2, Scer\GAL4^Act5C.PP, Wee1^UAS.cPa has cell lethal | partially phenotype

Dp^a4/Dp^a2 has wing disc | wandering third instar larval stage phenotype, suppressible by Df(3L)X14/+

Dp^a4/Dp^a2 has wing disc | wandering third instar larval stage phenotype, suppressible by Df(3L)XR38/+

Dp^a4/Dp^a2 has wing disc | wandering third instar larval stage phenotype, suppressible by Dark^CD4/Ark[+]

Dp^a1/Dp^a2 is a suppressor of mitotic cell cycle phenotype of Rheb^UAS.cSa, Scer\GAL4^Act5C.PP

Dp^a1/Dp^a2 is a suppressor of mitotic cell cycle phenotype of Myc^UAS.cZa, Scer\GAL4^Act5C.PP

Dp^a1/Dp^a2, Scer\GAL4^Act5C.PP, dap^UAS.cdNa has mitotic cell cycle phenotype

Dp^a1/Dp^a2, Scer\GAL4^Act5C.PP, Wee1^UAS.cPa has mitotic cell cycle phenotype