FlyBase Allele Report: Dmel\Wee1[UAS.cPa]

General Information

Symbol

Dmel\Wee1^UAS.cPa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Price

FlyBase ID

FBal0138116

Feature type

allele

Associated gene

Dmel\Wee1

Associated Insertion(s)

Carried in Construct

P{UAS-Wee1.P}

Also Known As

UAS-Dwee1, UAS-wee, UAS-Wee1

Key Links

Transgenic product class

cDNA

(Price et al., 2002)

wild_type

(Price et al., 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Price et al., 2002)

wild_type

(Price et al., 2002)

Mutagen

in vitro construct

(Price et al., 2002)

Progenitor genotype

Carried in construct

P{UAS-Wee1.P}

Cytology

Description

UASt regulatory sequences drive expression of a 2.2kb Wee1 cDNA fragment.

(Price et al., 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Wee1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle, with Scer\GAL4^en.PU

(Zielke et al., 2014)

abnormal mitotic cell cycle, with Scer\GAL4^unspecified

(Reis and Edgar, 2004)

visible, with Scer\GAL4^ap-md544

(Price et al., 2002)

visible, with Scer\GAL4^GMR.PF

(Price et al., 2002)

visible, with Scer\GAL4^pnr-MD237

(Price et al., 2002)

visible, with Scer\GAL4^sd-SG29.1

(Price et al., 2002)

Phenotype Manifest In

adult thorax, with Scer\GAL4^pnr-MD237

(Price et al., 2002)

chaeta & adult thorax | dorsal, with Scer\GAL4^ap-md544

(Price et al., 2002)

chaeta & adult thorax | dorsal, with Scer\GAL4^pnr-MD237

(Price et al., 2002)

interommatidial bristle, with Scer\GAL4^GMR.PF

(Price et al., 2002)

mitotic cell cycle, with Scer\GAL4^unspecified

(Reis and Edgar, 2004)

ommatidium, with Scer\GAL4^GMR.PF

(Price et al., 2002)

scutellum, with Scer\GAL4^ap-md544

(Price et al., 2002)

wing, with Scer\GAL4^ap-md544

(Price et al., 2002)

wing margin, with Scer\GAL4^sd-SG29.1

(Price et al., 2002)

Detailed Description

Statement

Reference

Scer\GAL4^en.PU-mediated expression increases the fraction of wing disc cells in G2 phase and concomitantly decreases the fraction in G1.

(Zielke et al., 2014)

Cells expressing wee^Scer\UAS.cPa show an elongated G2 phase, their rates of division are similar to wild-type indicating their G1 phases are shorter.

(Reis and Edgar, 2004)

Expression of wee^Scer\UAS.cPa under the control of one copy of Scer\GAL4^ap-md544 or Scer\GAL4^pnr-MD237 results in a reduced number of sensory bristles on the adult dorsal thorax. Flies expressing wee^Scer\UAS.cPa under the control of two copies of Scer\GAL4^ap-md544 have a nearly bald dorsal thorax and a severe reduction of the scutellum. Flies expressing wee^Scer\UAS.cPa under the control of two copies of Scer\GAL4^pnr-MD237 have a furrowed thorax. Flies expressing wee^Scer\UAS.cPa under the control of Scer\GAL4^ap-md544 have upturned wings. Flies expressing wee^Scer\UAS.cPa under the control of Scer\GAL4^sd-SG29.1 show extensive scalloping of the wing margin. Flies expressing wee^Scer\UAS.cPa under the control of Scer\GAL4^GMR.PF have disorganised facets and frequent duplications of bristles in the eye.

(Price et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^sd-SG29.1, Wee1^UAS.cPa has visible phenotype, enhanceable by Scer\GAL4^sd-SG29.1/trbl^UAS.cGa

(Price et al., 2002)

Scer\GAL4^sd-SG29.1, Wee1^UAS.cPa has visible phenotype, enhanceable by trbl^UAS.cGa/Scer\GAL4^GMR.PF

(Price et al., 2002)

Enhancer of

Statement

Reference

Scer\GAL4^sd-SG29.1/Wee1^UAS.cPa is an enhancer of visible phenotype of Scer\GAL4^sd-SG29.1, trbl^UAS.cGa

(Price et al., 2002)

Wee1^UAS.cPa/Scer\GAL4^GMR.PF is an enhancer of visible phenotype of Scer\GAL4^sd-SG29.1, trbl^UAS.cGa

(Price et al., 2002)

Other

Statement

Reference

Scer\GAL4^neur-P72, Wee1^UAS.cPa, stg^AR2 has decreased occurrence of cell division phenotype

(Fichelson and Gho, 2004)

Dp^a1/Dp^a2, Scer\GAL4^Act5C.PP, Wee1^UAS.cPa has abnormal mitotic cell cycle phenotype

(Reis and Edgar, 2004)

Dp^a1/Dp^a2, Scer\GAL4^Act5C.PP, Wee1^UAS.cPa has cell lethal | partially phenotype

(Reis and Edgar, 2004)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^sd-SG29.1, Wee1^UAS.cPa has wing phenotype, enhanceable by Scer\GAL4^sd-SG29.1/trbl^UAS.cGa

(Price et al., 2002)

Scer\GAL4^sd-SG29.1, Wee1^UAS.cPa has eye phenotype, enhanceable by trbl^UAS.cGa/Scer\GAL4^GMR.PF

(Price et al., 2002)

Scer\GAL4^sd-SG29.1, Wee1^UAS.cPa has interommatidial bristle phenotype, enhanceable by trbl^UAS.cGa/Scer\GAL4^GMR.PF

(Price et al., 2002)

Enhancer of

Statement

Reference

Scer\GAL4^sd-SG29.1/Wee1^UAS.cPa is an enhancer of wing phenotype of Scer\GAL4^sd-SG29.1, trbl^UAS.cGa

(Price et al., 2002)

Wee1^UAS.cPa/Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Scer\GAL4^sd-SG29.1, trbl^UAS.cGa

(Price et al., 2002)

Wee1^UAS.cPa/Scer\GAL4^GMR.PF is an enhancer of interommatidial bristle phenotype of Scer\GAL4^sd-SG29.1, trbl^UAS.cGa

(Price et al., 2002)

Other

Statement

Reference

Scer\GAL4^neur-P72, Wee1^UAS.cPa, stg^AR2 has macrochaeta phenotype

(Fichelson and Gho, 2004)

Scer\GAL4^neur-P72, Wee1^UAS.cPa, stg^AR2 has trichogen cell phenotype

(Fichelson and Gho, 2004)

Scer\GAL4^neur-P72, Wee1^UAS.cPa, stg^AR2 has tormogen cell phenotype

(Fichelson and Gho, 2004)

Scer\GAL4^neur-P72, Wee1^UAS.cPa, stg^AR2 has external sensory organ precursor cell I phenotype

(Fichelson and Gho, 2004)

Dp^a1/Dp^a2, Scer\GAL4^Act5C.PP, Wee1^UAS.cPa has mitotic cell cycle phenotype

(Reis and Edgar, 2004)

Additional Comments

Genetic Interactions

Statement

Reference

When wee^Scer\UAS.cPa is driven by Scer\GAL4^neur-P72 in stg^AR2 animals exhibit severe bristle loss. The lineage of external sensory organ is significantly modified in mutants. Usually, only two cell divisions of the pI cell are seen as opposed to the three seen in wild-type. The first mitosis, oriented in the epithelial plane, gives rise to a large anterior cell and a small posterior glial cell. The glial cell quickly undergoes nuclear fragmentation. The large anterior cell divides once and resulting in a sheath and neuron cell. Also the timing of cell divisions is stringly affected. In the median rows od sensory organs, the first division occurs on average 4 hours later than in wild-type. The second division occurs ~3 hours late. Finally the polarity of the first division is reversed compared to the wild-type situation. This abnormal division sequence is observed in 78% of mutant organs.

(Fichelson and Gho, 2004)

Cells expressing wee^Scer\UAS.cPa driven by Scer\GAL4^Act5C.PP in a Dp^a1/Dp^a2 background, divide more slowly than controls. These cells are also subviable.

(Reis and Edgar, 2004)

Coexpression of wee^Scer\UAS.cPa and trbl^Scer\UAS.cGa under the control of Scer\GAL4^sd-SG29.1 results in almost complete ablation of wing tissue. Occasionally, conversion of wing tissue to thoracic tissue is seen.

(Price et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Wee1^Scer\UAS.cPa

Wee1^UAS.cPa

wee^Scer\UAS.cPa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Price

(Price et al., 2002)

Secondary FlyBase IDs

References (8)

Report Sections

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