FlyBase Allele Report: Dmel\Dp[a1]

General Information

Symbol

Dmel\Dp^a1

Species

D. melanogaster

Name

FlyBase ID

FBal0063499

Feature type

allele

Associated gene

Dmel\Dp

Associated Insertion(s)

Carried in Construct

Also Known As

dDP^a1

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Royzman et al., 1997)

Progenitor genotype

Cytology

Description

Mutation is within the DEF box in a block of amino acids that is highly conserved between Dp and E2f1.

(Royzman et al., 1997)

Nucleotide substitution: C?T.

(Royzman et al., 1997)

Amino acid replacement: R149C.

(Royzman et al., 1997)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:13,226,234..13,226,234 [+]

Nucleotide change:

C13226234T

Amino acid change:

R217C | Dp-PA; R213C | Dp-PB; R189C | Dp-PC

(Royzman et al., 1997)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

egg chorion

(Royzman et al., 1999)

eye

(Royzman et al., 1997)

eye (with Dp^a2)

(Royzman et al., 1997)

follicle cell (with Df(2R)vg56)

(Cayirlioglu et al., 2003)

follicle cell & nucleus

(Royzman et al., 1999)

heterochromatin & nurse cell (with Df(2R)vg56)

(Royzman et al., 2002)

interommatidial bristle

(Royzman et al., 1997)

interommatidial bristle (with Dp^a2)

(Royzman et al., 1997)

macrochaeta

(Royzman et al., 1997)

nuclear membrane & nurse cell (with Df(2R)vg56)

(Royzman et al., 2002)

nurse cell (with Df(2R)vg56)

(Royzman et al., 2002)

ommatidium

(Royzman et al., 1997)

ommatidium (with Dp^a2)

(Royzman et al., 1997)

ovary (with Df(2R)vg56)

(Royzman et al., 2002)

polar body nucleus (with Df(2R)vg56)

(Royzman et al., 2002)

tergite

(Royzman et al., 1997)

wing vein

(Royzman et al., 1997)

Detailed Description

Statement

Reference

Dp^a1/Df(2R)vg56 follicle cells show increased ploidy compared to control cells by FACS analysis.

(Cayirlioglu et al., 2003)

The ovaries of Dp^a1/Df(2R)vg56 females are smaller than wild-type but have the normal complement of nurse and follicle cells. Occasionally, degenerating early egg chambers (stage 8 and earlier) are seen. The predominant defects in Dp^a1/Df(2R)vg56 females are a failure of nurse cells to deposit their contents into the oocyte and degenerate and an increased amount of heterochromatic DNA in the nurse cell nuclei. In homozygous Dp^a1 egg chambers, unlike wild-type (where nurse cells undergo apoptosis during stages 12 to 13 and have degenerated by stage 14), degeneration of nurse cells is abnormal and occurs late if at all. Dp^a1/Df(2R)vg56 mutant mothers lay few eggs. In the rare cases in which mature eggs are laid most are unfertilized, apparently due to defects in chorion structure. The few fertilized embryos arrest in early division cycles. In addition, the morphology of the polar bodies are affected. In embryos from these females meiosis is completed, but polar body chromosomes are either condensed, improperly arranged or dispersed in the embryo.

(Royzman et al., 2002)

Eggs laid by hemizygous females have thin eggshells. Hemizygous ovaries show abnormal patterns of BrdU incorporation during stage 10B and later stages. The follicle cell nuclei fail to initiate amplification in most egg chambers, while in some stage 10B egg chambers genomic replication rather than amplification is seen.

(Royzman et al., 1999)

Disrupt G[[1]]-S transcription late in embryogenesis, DNA replication still occurs.

Hemizygotes exhibit rough eyes, incomplete wing vein, thin and short bristles and etched tergites. Females lay eggs with thin chorions. More females than males in progeny.

Dp^a1/Dp^a2 flies exhibit reduced eyes, missing and disorganised ommatidia, stunted, missing and disorganised bristles. Daily heat shocks of Dp^hs.PD rescued proliferation defects to normal looking eyes.

Heat induced expression of E2f1^hs.PD at 25[o]C provides significant rescue of the mutant phenotype, but higher levels of expression dampen the rescue.

(Royzman et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Dp^a1 is an enhancer of visible phenotype of Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH

(Gupta et al., 2008)

Dp^a1 is an enhancer of abnormal size phenotype of Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH

(Gupta et al., 2008)

Suppressor of

Statement

Reference

Dp^a1/Dp^a2 is a suppressor of abnormal mitotic cell cycle phenotype of Rheb^UAS.cSa, Scer\GAL4^Act5C.PP

(Reis and Edgar, 2004)

Dp^a1/Dp^a2 is a suppressor of abnormal mitotic cell cycle phenotype of Myc^UAS.cZa, Scer\GAL4^Act5C.PP

(Reis and Edgar, 2004)

Other

Statement

Reference

Dp^a1/Dp^a2, Scer\GAL4^Act5C.PP, dap^UAS.cdNa has abnormal mitotic cell cycle phenotype

(Reis and Edgar, 2004)

Dp^a1/Dp^a2, Scer\GAL4^Act5C.PP, Wee1^UAS.cPa has abnormal mitotic cell cycle phenotype

(Reis and Edgar, 2004)

Dp^a1/Dp^a2, Scer\GAL4^Act5C.PP, Wee1^UAS.cPa has cell lethal | partially phenotype

(Reis and Edgar, 2004)

Phenotype Manifest In

Enhancer of

Statement

Reference

Dp^a1 is an enhancer of eye phenotype of Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH

(Gupta et al., 2008)

Suppressor of

Statement

Reference

Dp^a1/Dp^a2 is a suppressor of mitotic cell cycle phenotype of Rheb^UAS.cSa, Scer\GAL4^Act5C.PP

(Reis and Edgar, 2004)

Dp^a1/Dp^a2 is a suppressor of mitotic cell cycle phenotype of Myc^UAS.cZa, Scer\GAL4^Act5C.PP

(Reis and Edgar, 2004)

Other

Statement

Reference

Dp^a1/Dp^a2, Scer\GAL4^Act5C.PP, dap^UAS.cdNa has mitotic cell cycle phenotype

(Reis and Edgar, 2004)

Dp^a1/Dp^a2, Scer\GAL4^Act5C.PP, Wee1^UAS.cPa has mitotic cell cycle phenotype

(Reis and Edgar, 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Cells expressing dap^{Scer\UAS.cdNa} driven by Scer\GAL4^Act5C.PP in a Dp^a1/Dp^a2 background, divide more slowly than controls. Cells expressing wee^Scer\UAS.cPa driven by Scer\GAL4^Act5C.PP in a Dp^a1/Dp^a2 background, divide more slowly than controls. These cells are also subviable. Cells expressing dm^Scer\UAS.cZa or Rheb^Scer\UAS.cSa driven by Scer\GAL4^Act5C.PP in a Dp^a1/Dp^a2 background, the compensatory elongation of the G2 phase, is not as pronounced seen in dm^Scer\UAS.cZa expressing cells.

(Reis and Edgar, 2004)

Xenogenetic Interactions

Statement

Reference

Dp^a1 enhances the eye phenotypes seen when Mmus\Shisa5^Scer\UAS.cGa is expressed under the control of Scer\GAL4^dpp.PH.

(Gupta et al., 2008)

Complementation and Rescue Data

Rescued by

Dp^a1 is rescued by Dp^hs.PD

(Royzman et al., 1997)

Comments

Daily heat shocks of Dp^hs.PD in Dp^a1/Dp^a2 individuals rescues the proliferation defects to normal looking eyes.

Heat induced expression of Dp^hs.PD at 25^oC partially rescues the lethality, males are fertile. Recovered adults still exhibit the visible eye, wing vein and bristle defects and are still female sterile. Induced expression at 37^oC completely rescues the lethality and all developmental phenotypes are suppressed. Daily heat shocks of Dp^hs.PD rescued proliferation defects seen in Dp^a1/Dp^a2 flies leading to normal looking eyes.

(Royzman et al., 1997)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

7277

w^*; cn¹ Dp^a1 bw¹ speck¹/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Dp^a1

(Flegel et al., 2016, Baumgardt et al., 2014, Gupta et al., 2008, Christensen and Cook, 2007.3.22, Christensen and Cook, 2007.3.22, Cayirlioglu et al., 2003)

dDP^a1

(Reis and Edgar, 2004, Royzman et al., 2002, Bosco et al., 2001, Myster et al., 2000, Royzman et al., 1999)

dDp^a1

(Royzman and Orr-Weaver, 1998, Royzman et al., 1997)

Name Synonyms

Secondary FlyBase IDs

References (13)

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