FlyBase Allele Report: Dmel\Taf6[XS-922]

General Information

Symbol

Dmel\Taf6^XS-922

Species

D. melanogaster

Name

FlyBase ID

FBal0048985

Feature type

allele

Associated gene

Dmel\Taf6

Associated Insertion(s)

Carried in Construct

Also Known As

TAF60^XS-922

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

X ray

Nature of the Allele

Allele class

loss of function allele

(Aoyagi and Wassarman, 2001)

Mutagen

X ray

(Karim et al., 1996)

Progenitor genotype

Cytology

Description

In frame insertion of 6bp (TACTAC), which encodes two Tyr residues, adjacent to position 207 of the protein.

(Aoyagi and Wassarman, 2001)

In frame insertion of six base pairs that encodes two Tyr residues adjacent to position 207.

(Sauer et al., 1996)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

insertion

3L:19,599,828..19,599,829 [+]

Comment:

In frame insertion of 6bp (TACTAC).

Inserted_sequence:

TACTAC

(Aoyagi and Wassarman, 2001)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell number | adult stage, with Taf6^hs.Tag:FLAG

(Aoyagi and Wassarman, 2001)

cell lethal | recessive | somatic clone

(Aoyagi and Wassarman, 2001)

decreased cell number | germline clone | oogenesis

(Aoyagi and Wassarman, 2001)

decreased cell number | spermatogenesis (with Taf6²), with Taf6^hs.Tag:FLAG

(Aoyagi and Wassarman, 2001)

female sterile | germline clone | recessive

(Aoyagi and Wassarman, 2001)

increased cell size | adult stage, with Taf6^hs.Tag:FLAG

(Aoyagi and Wassarman, 2001)

lethal | recessive

(Karim et al., 1996, Sauer et al., 1996)

lethal - all die before end of larval stage (with Taf6¹)

(Aoyagi and Wassarman, 2001)

lethal - all die before end of larval stage (with Taf6³)

(Aoyagi and Wassarman, 2001)

lethal - all die before end of larval stage | recessive

(Aoyagi and Wassarman, 2001)

lethal - all die before end of pupal stage (with Taf6²)

(Aoyagi and Wassarman, 2001)

male sterile (with Taf6²), with Taf6^hs.Tag:FLAG

(Aoyagi and Wassarman, 2001)

some die during larval stage (with Taf6¹)

(Aoyagi and Wassarman, 2001)

some die during larval stage (with Taf6³)

(Aoyagi and Wassarman, 2001)

some die during larval stage | recessive

(Aoyagi and Wassarman, 2001)

some die during pupal stage (with Taf6²)

(Aoyagi and Wassarman, 2001)

visible (with Taf6²), with Taf6^hs.Tag:FLAG

(Aoyagi and Wassarman, 2001)

visible (with Taf6^hs.Tag:FLAG)

(Aoyagi and Wassarman, 2001)

Phenotype Manifest In

eye (with Taf6²), with Taf6^hs.Tag:FLAG

(Aoyagi and Wassarman, 2001)

eye (with Taf6^hs.Tag:FLAG)

(Aoyagi and Wassarman, 2001)

eye photoreceptor cell (with Taf6^hs.Tag:FLAG)

(Aoyagi and Wassarman, 2001)

eye photoreceptor cell | increased number (with Taf6^hs.Tag:FLAG)

(Aoyagi and Wassarman, 2001)

ommatidium (with Taf6^hs.Tag:FLAG)

(Aoyagi and Wassarman, 2001)

oocyte | germline clone

(Aoyagi and Wassarman, 2001)

primary spermatocyte (with Taf6²), with Taf6^hs.Tag:FLAG

(Aoyagi and Wassarman, 2001)

rhabdomere (with Taf6^hs.Tag:FLAG)

(Aoyagi and Wassarman, 2001)

spermatid (with Taf6²), with Taf6^hs.Tag:FLAG

(Aoyagi and Wassarman, 2001)

spermatocyte, with Taf6^hs.Tag:FLAG

(Aoyagi and Wassarman, 2001)

testis (with Taf6²), with Taf6^hs.Tag:FLAG

(Aoyagi and Wassarman, 2001)

wing (with Taf6²), with Taf6^hs.Tag:FLAG

(Aoyagi and Wassarman, 2001)

wing (with Taf6^hs.Tag:FLAG)

(Aoyagi and Wassarman, 2001)

Detailed Description

Statement

Reference

Homozygous female germline clones fail to produce eggs.

Homozygous clones generated in the eye are not recovered (although twin spots are seen).

Taf6^XS-922 homozygotes rescued to adulthood by expression of Taf6^{hs.T:Zzzz\FLAG} have an external rough eye phenotype. The ommatidia have an abnormal number of photoreceptors in many cases, either missing an inner or outer photoreceptor or having an extra inner or outer photoreceptor. The mutant rhabdomeres are larger than normal in the mutant animals. The mutant adults have a notched wing phenotype.

Taf6^XS-922/Taf6² animals rescued to adulthood by expression of Taf6^{hs.T:Zzzz\FLAG} have notched wing and rough eye phenotypes, which are less severe if the animals are heat shocked during adulthood to express Taf6^{hs.T:Zzzz\FLAG}.

Taf6^XS-922/Taf6² males rescued to adulthood by expression of Taf6^{hs.T:Zzzz\FLAG} (including heat shock during the adult stage) have postmeiotic stages including mature spermatids in the testis, but they are less abundant than in wild type. Taf6^XS-922/Taf6² males rescued to adulthood by expression of Taf6^{hs.T:Zzzz\FLAG} with no heat shock during the adult stage have a more severe phenotype; proliferation of stem cells to primary spermatocytes is normal, but the primary spermatocytes occupy an abnormally large portion of the testis and degenerate without initiating meiotic chromosome condensation.

Taf6^XS-922 homozygous males rescued to adulthood by expression of Taf6^{hs.T:Zzzz\FLAG} have needle-like structures in the spermatocytes.

(Aoyagi and Wassarman, 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Taf6^XS-922 is an enhancer of visible phenotype of Dsor1^XS520

(Karim et al., 1996)

Suppressor of

Statement

Reference

Taf6[+]/Taf6^XS-922 is a suppressor of visible phenotype of Ras85D^V12.sev

(Aoyagi and Wassarman, 2001)

Taf6[+]/Taf6^XS-922 is a suppressor of decreased cell number | adult stage phenotype of Ras85D^V12.sev

(Aoyagi and Wassarman, 2001)

Taf6^XS-922 is a suppressor of lethal phenotype of Ras85D^V12.sev, sev^S11.Tag:MYC

(Maixner et al., 1998)

Other

Statement

Reference

Ras85D^V12.sev, Taf6^XS-922, sev^S11.Tag:MYC has viable phenotype

(Maixner et al., 1998)

Phenotype Manifest In

Enhancer of

Statement

Reference

Taf6^XS-922 is an enhancer of eye phenotype of Dsor1^XS520

(Karim et al., 1996)

Taf6^XS-922 is an enhancer of eye phenotype of Raf¹²

(Karim et al., 1996)

NOT Enhancer of

Statement

Reference

Taf6^XS-922 is a non-enhancer of embryo phenotype of bcd^ΔQAC, bcd⁶

(Schaeffer et al., 1999)

Suppressor of

Statement

Reference

Taf6[+]/Taf6^XS-922 is a suppressor of eye phenotype of Ras85D^V12.sev

(Aoyagi and Wassarman, 2001)

Taf6[+]/Taf6^XS-922 is a suppressor of photoreceptor cell R7 phenotype of Ras85D^V12.sev

(Aoyagi and Wassarman, 2001)

Taf6^XS-922 is a suppressor of phenotype of Ras85D^V12.sev

(Karim et al., 1996, Sauer et al., 1996)

NOT Suppressor of

Statement

Reference

Taf6^XS-922 is a non-suppressor of embryo phenotype of bcd^ΔQAC, bcd⁶

(Schaeffer et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

The rough eye phenotype of Ras85D^V12.sev flies is dominantly suppressed by Taf6^XS-922. The increased number of R7 photoreceptor cells per ommatidium that is seen in Ras85D^V12.sev flies is suppressed from 3.1 to 1.5 by Taf6^XS-922/+.

(Aoyagi and Wassarman, 2001)

The ability of bcd^ΔQAC to rescue embryos derived from bcd⁶ females is not affected by Taf6^XS-922.

(Schaeffer et al., 1999)

The lethality caused by sev^{S11.T:Hsap\MYC} in combination with Ras85D^V12.sev is suppressed by Taf6^XS-922.

(Maixner et al., 1998)

Suppresses the rough eye phenotype of Ras85D^V12.sev. Has little or no effect on the phenotype of Ras85D^N17.sev. Has little or no effect on the phenotype of phl::tor^13D.hs.sev. Suppresses the phenotype of Ras85D::Src64B^{V12.ΔCAAX.sev}. Has little or no effect on the lethality of phl¹². Enhances the phl¹² eye phenotype. Enhances the Dsor1^XS520 eye phenotype. Has little or no effect on the aop^S2382 eye phenotype.

(Karim et al., 1996)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

Taf6³/Taf6^XS-922 is partially rescued by Taf6^hs.Tag:FLAG

(Aoyagi and Wassarman, 2001)

Taf6¹/Taf6^XS-922 is partially rescued by Taf6^hs.Tag:FLAG

(Aoyagi and Wassarman, 2001)

Taf6²/Taf6^XS-922 is partially rescued by Taf6^hs.Tag:FLAG

(Aoyagi and Wassarman, 2001)

Taf6^XS-922 is partially rescued by Taf6^hs.Tag:FLAG

(Aoyagi and Wassarman, 2001)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Insertion disrupts an evolutionarily conserved region of the protein (amino acids 195-452).

(Sauer et al., 1996)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

SR3-4B^XS-922

(Sauer et al., 1996)

TAF60^XS-922

(Maixner et al., 1998)

TAF60YY

(Pham et al., 1999)

TAF_II60^XS922

(Aoyagi and Wassarman, 2001)

TAF_II60^YY

(Schaeffer et al., 1999, Sauer et al., 1998)

TAF_II60YY

(Zhou et al., 1998)

Taf60^XS-922

(Karim et al., 1996)

Taf6^XS-922

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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