FlyBase Allele Report: Dmel\Pka-C1[H2]

The class IV dendritic arborization neurons in Pka-C1^H2 mutant clones show a significant decrease in the complexity of dendritic arbors in respect to the total dendritic length. The total number of dendritic branching points is also decreased.

(Copf, 2014)

Pka-C1^H2 heterozygotes do not exhibit an effect on immediate memory and long-term memory.

(Zhao et al., 2013)

No change in wing area is seen in heterozygous Pka-C1^H2 mutant flies raised at 27[o]C.

(Cheng et al., 2012)

Pka-C1^H2/+ heterozygotes have dramatically improved memory retention curves compared with wild-type flies. Avoidance of naive flies to the odors odors and electrical shocks used during training is not significantly different between wild-type and Pka-C1^H2 heterozygous mutants, indicating that the observed increases in memory are not caused by increased sensitivity to these stimuli. Memory retention curves of Pka-C1^H2 /+ heterozygotes indicate that early forms of memory, including memory tested immediately after training (3-min memory) and short forms of memory (1-h memory) are not greatly affected. However, memory at later time points, 3 h and 7 h after training, progressively increases relative to wild-type, such that at 7h, memory is approximately double that of wild-type.

Pka-C1^H2/+ flies have improved cold shock-resistant 3-h memory, indicating that anesthesia-resistant memory is increased in these flies. Pka-C1^H2/+ heterozygote show increased 24-h memory after massed training, indicating increased ARM production.

Compared to wild-types, long-term memory is not altered in Pka-C1^H2/+ heterozygotes.

Memory enhancement in Pka-C1^H2/+ flies begins between 1 and 3 h after single cycle training and reverts to normal within 4 days after spaced training.

(Horiuchi et al., 2008)

Heterozygotes show normal one-day memory after spaced training in a Pavlovian olfactory learning assay.

(Lu et al., 2007)

Pka-C1^H2 germline clones show over 90% penetrance of mid-oogenesis AP polarity defects and fusion of nurse cell membranes. The polarity defects are in least in part due to a defect in microtubule organization; microtubules emanate from along the entire cortex in stage 8-10 oocytes, instead of emanating from multiple sites at the anterior cortex only as in wild-type oocytes. Additionally, a strong focus of microtubules is sometimes seen at the posterior pole of these clones, indicating that these oocytes aberrantly retain a discrete posterior microtubule organizing center. However, movement of the oocyte nucleus from the posterior to the anterior cortex is not affected and dorsal follicle cell fate is specified correctly.

(Steinhauer and Kalderon, 2005)

When mutant clones are made in the legs of female adults, slightly thickened bristles are seen, indicating a weak transformation to male.

(Horabin et al., 2003)

Egg chambers containing homozygous follicle cell clones contain ectopic polar cells.

(Bai and Montell, 2002)

Germ cells in embryos lacking maternal Pka-C1 function (derived from females carrying Pka-C1^H2 germline clones and fertilised with a wild-type sperm) associate prematurely into clumps at stage 11 and remain in clumps at the centre of the embryo at later stages. There is an increase in germ cell number indicative of a failure to maintain cell cycle arrest. These embryos do not hatch.

(Deshpande et al., 2001)

Homozygous somatic stem cell clones in the ovary induce ectopic polar and border cells, but cause only mild over-proliferation of follicle cells and do not affect oocyte positioning or oocyte polarity (as indicated by normal migration of the oocyte nucleus to the dorsal anterior corner). Mutant ovarioles include follicle cells that proliferate beyond stage 6 (in contrast to wild type), although rarely beyond stage 8.

(Zhang and Kalderon, 2000)

Heterozygotes show increased sensitivity to ethanol in an inebriometer assay.

(Moore et al., 1998)

Clones in the eye that lack both Pka-C1 and smo behave like loss of function Pka-C1 clones. Clones show ectopic photoreceptor differentiation and eventually merge with the endogenous field of differentiation, show no retardation of the furrow, pass through a furrow fate and induce non-autonomous ectopic photoreceptor differentiation outside the clone.

(Strutt and Mlodzik, 1997)

Homozygous clones produce anterior duplications of the normal pattern in the wing. Reduction or elimination of protein kinase A activity has no effect on the phenotype generated when the G-sα60A pathway is activated in wing epithelial cells; wing duplications that contain blisters are formed when G-sα60A^{Q215L.Scer\UAS} is expressed in a Pka-C1^H2 background using Scer\GAL4^OK10.

(Wolfgang et al., 1996)

Border follicle cells sometimes do not migrate or show incomplete migration in Pka-C1^H2/Pka-C1^Tw2 females.

(Lane and Kalderon, 1995)

Pka-C1^H2 clones cause pattern defects in the wing, notum, halteres, antennae and leg.

(Li et al., 1995)

Homozygous germline clones generate egg chambers with numerous nurse cell fusions.

(Lane and Kalderon, 1994)

Hemizygotes are larval lethal. Mature oocytes are smaller than wild type and nurse cells are multinucleate.

(Lane and Kalderon, 1993)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Pka-C1^H2 has lethal phenotype, non-suppressible by Mmus\Prkaca^mC.Act5C

(Steinhauer and Kalderon, 2005)

Suppressor of

Statement

Reference

Pka-C1^H2 is a suppressor of increased size | larval stage phenotype of mthl5^MB03076

(Saad and Hipfner, 2021)

Pka-C1[+]/Pka-C1^H2 is a suppressor of partially lethal - majority die | maternal effect phenotype of Pka-R1¹⁸³⁰⁴

(Yoshida et al., 2004)

Other

Statement

Reference

Pka-C1[+]/Pka-C1^H2, rg¹ has abnormal memory phenotype

(Zhao et al., 2013)

Pka-C1^H2, rg[+]/rg¹ has abnormal memory phenotype

(Zhao et al., 2013)

Pka-C1[+]/Pka-C1^H2, spoon^P1 has abnormal memory | dominant phenotype

(Lu et al., 2007)

Pka-C1^H2, yu[+]/spoon^P1 has abnormal memory | dominant phenotype

(Lu et al., 2007)

Pka-C1^H2, Su(fu)^LP has abnormal cell polarity | somatic clone | oogenesis phenotype

(Zhang and Kalderon, 2000)

Pka-C1^H2, Su(fu)^LP has increased cell number | somatic clone | oogenesis phenotype

(Zhang and Kalderon, 2000)

Pka-C1^H2, Su(fu)^LP has increased occurrence of cell division | somatic clone | oogenesis phenotype

(Zhang and Kalderon, 2000)

Phenotype Manifest In

Enhanced by

Statement

Reference

Pka-C1^H2 has phenotype, enhanceable by Su(fu)^LP/Su(fu)^LP

(Zhang and Kalderon, 2000)

Pka-C1^H2 has follicle cell | ectopic | somatic clone phenotype, enhanceable by Su(fu)^LP/Su(fu)^LP

(Zhang and Kalderon, 2000)

Suppressed by

Statement

Reference

Pka-C1^H2 has nurse cell | germline clone phenotype, suppressible by Mmus\Prkaca^mC.Act5C

(Steinhauer and Kalderon, 2005)

Pka-C1^H2 has oocyte | germline clone phenotype, suppressible by Mmus\Prkaca^mC.Act5C

(Steinhauer and Kalderon, 2005)

Pka-C1^H2 has wing | somatic clone phenotype, suppressible by Mmus\Prkaca^mC.Act5C

(Li et al., 1995)

Pka-C1^H2 has scutum | somatic clone phenotype, suppressible by Mmus\Prkaca^mC.Act5C

(Li et al., 1995)

Pka-C1^H2 has haltere | somatic clone phenotype, suppressible by Mmus\Prkaca^mC.Act5C

(Li et al., 1995)

Pka-C1^H2 has antenna | somatic clone phenotype, suppressible by Mmus\Prkaca^mC.Act5C

(Li et al., 1995)

Pka-C1^H2 has wing | somatic clone phenotype, suppressible by dpp^H61

(Li et al., 1995)

Pka-C1^H2 has scutum | somatic clone phenotype, suppressible by dpp^H61

(Li et al., 1995)

Pka-C1^H2 has haltere | somatic clone phenotype, suppressible by dpp^H61

(Li et al., 1995)

Pka-C1^H2 has antenna | somatic clone phenotype, suppressible by dpp^H61

(Li et al., 1995)

Pka-C1^H2 has leg | somatic clone phenotype, suppressible by dpp^H61

(Li et al., 1995)

Pka-C1^H2 has leg | somatic clone phenotype, suppressible by wg^l-17

(Li et al., 1995)

Pka-C1^H2 has wing | somatic clone phenotype, suppressible by dpp^H46

(Li et al., 1995)

Pka-C1^H2 has scutum | somatic clone phenotype, suppressible by dpp^H46

(Li et al., 1995)

Pka-C1^H2 has haltere | somatic clone phenotype, suppressible by dpp^H46

(Li et al., 1995)

Pka-C1^H2 has antenna | somatic clone phenotype, suppressible by dpp^H46

(Li et al., 1995)

Pka-C1^H2 has leg | somatic clone phenotype, suppressible by dpp^H46

(Li et al., 1995)

Pka-C1^H2 has wing | somatic clone phenotype, suppressible by wg^L2

(Li et al., 1995)

Pka-C1^H2 has scutum | somatic clone phenotype, suppressible by wg^L2

(Li et al., 1995)

Pka-C1^H2 has haltere | somatic clone phenotype, suppressible by wg^L2

(Li et al., 1995)

Pka-C1^H2 has antenna | somatic clone phenotype, suppressible by wg^L2

(Li et al., 1995)

Pka-C1^H2 has leg | somatic clone phenotype, suppressible by wg^L2

(Li et al., 1995)

NOT suppressed by

Statement

Reference

Pka-C1^H2 has nurse cell | germline clone phenotype, non-suppressible by Lkb1^{S535E.UASp.GFP}/Scer\GAL4^unspecified

(Steinhauer and Kalderon, 2005)

Pka-C1^H2 has oocyte | germline clone phenotype, non-suppressible by Lkb1^{S535E.UASp.GFP}/Scer\GAL4^unspecified

(Steinhauer and Kalderon, 2005)

Enhancer of

Statement

Reference

Pka-C1[+]/Pka-C1^H2 is an enhancer of wing cell phenotype of Df(3R)Gprk2-del1, Gprk2^KO

(Cheng et al., 2012)

Suppressor of

Statement

Reference

Pka-C1^H2 is a suppressor of wing disc | larval stage phenotype of mthl5^MB03076

(Saad and Hipfner, 2021)

Pka-C1[+]/Pka-C1^H2 is a suppressor of embryonic abdominal segment | germline clone | maternal effect phenotype of Pka-R1^E1/Pka-R1¹⁸³⁰⁴

(Yoshida et al., 2004)

Pka-C1[+]/Pka-C1^H2 is a suppressor of embryonic head | germline clone | maternal effect phenotype of Pka-R1^E1/Pka-R1¹⁸³⁰⁴

(Yoshida et al., 2004)

Pka-C1^H2/Pka-C1^H2 is a suppressor of phenotype of hh^ts2

(Zhang and Kalderon, 2000)

Pka-C1^H2/Pka-C1^H2 is a suppressor of egg chamber phenotype of hh^ts2

(Zhang and Kalderon, 2000)

Pka-C1^H2 is a suppressor of egg chamber phenotype of hh^ts2

(Zhang and Kalderon, 2000)

Other

Statement

Reference

Pka-C1^H2, Su(fu)^LP has oocyte nucleus | somatic clone phenotype

(Zhang and Kalderon, 2000)

Pka-C1^H2, Su(fu)^LP has oocyte | somatic clone phenotype

(Zhang and Kalderon, 2000)

Additional Comments

Genetic Interactions

Statement

Reference

rg¹/+ ; Pka-C1^H2/+ double-heterozygous flies exhibit a short-term memory defect after one-cycle training, but display normal long-term memory after space training.

(Zhao et al., 2013)

One copy of Pka-C1^H2 enhances the reduction in L3-L4 intervein area seen in Gprk2^KO/Df(3R)Gprk2-del1 mutant flies.

(Cheng et al., 2012)

yu^P1/+ ; Pka-C1^H2/+ double heterozygotes show impaired one-day memory after spaced training in a Pavlovian olfactory learning assay.

(Lu et al., 2007)

Expression of the lkb1^{S535E.Scer\UAS.P\T.T:Avic\GFP} transgene fails to rescue the oocyte polarity and nurse cell fusion phenotypes of Pka-C1^H2 germline clones.

(Steinhauer and Kalderon, 2005)

Somatic stem cell clones in the ovary homozygous for both Pka-C1^H2 and Su(fu)^LP induce extensive follicle cell over proliferation, mis-placed oocytes (28%) and aberrant oocyte polarity (28%). The egg chamber proliferation defects of hh^ts2 females kept at the restrictive temperature are rescued by homozygous Pka-C1^H2 clones, producing fully developed ovarioles in some cases. In all cases, the proliferating somatic cells are homozygous for Pka-C1^H2.

(Zhang and Kalderon, 2000)

Homozygous Pka-C1^H2 female germ line clones that also carry Mmus\Pkaca^mC.Act5C give rise to eggs which can be fertilised and develop in a small number of cases. Many Pka-C1^H2/Df(2L)γ15 embryos derived from these germ line clones hatch, and have ventral cuticle patterns similar to wild-type.

(Ohlmeyer and Kalderon, 1997)

The pattern defects seen in Pka-C1^H2 clones can be suppressed by expression of dpp^H46, dpp^H61, wg^l-17 and wg^L2.

(Li et al., 1995)

Xenogenetic Interactions

Statement

Reference

Expression of the constitutively active Mmus\Pkaca^mC.Act5C transgene rescues the oocyte polarity and nurse cell fusion phenotypes in Pka-C1^H2 germline clones. However, the transgene is unable to rescue the embryonic and larval lethality of Pka-C1^H2 mutants.

(Steinhauer and Kalderon, 2005)

The pattern defects seen in Pka-C1^H2 clones can be suppressed by expression of Mmus\Pkaca^mC.Act5C.

(Li et al., 1995)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

4101

107586

Notes on Origin

Discoverer

Comments

Strong Pka-C1 allele.

(Lane and Kalderon, 1993)

Clonal analysis indicates that Pka-C1 acts autonomously in the germline.

(Lane and Kalderon, 1995)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (15)

Reported As

Symbol Synonym

DC0^H2

(Horiuchi et al., 2008, Yamazaki et al., 2007, Horabin et al., 2003, Lefers et al., 2001, Wang and Holmgren, 2000, Zhang and Kalderon, 2000, Chen and Struhl, 1998, Ohlmeyer and Kalderon, 1997, Li et al., 1996, Melendez et al., 1995)

DC0^h2

(Huang and Kunes, 1998)

DCO^H2

(Yoshida et al., 2004, Bai and Montell, 2002, Vied and Horabin, 2001, Gorfinkiel et al., 1999, Moore et al., 1998, Li et al., 1995)

PKA-C1^H2

(Bala Tannan et al., 2018, Lu et al., 2007)

PKA^H2

(Copf, 2014)

PKA^h2

(Brennan et al., 2001)

PKAC1^H2

(Graves et al., 2015)

Pka-C1^H2

(Kahsai and Cook, 2018, Lee et al., 2018, Zhao et al., 2013)

Pka^H2

(Tanimoto et al., 2000)

PkaC1^H2

(Li et al., 2021)

dco^H2

(Wolfgang et al., 1996)

pka-C1^H2

(Saad and Hipfner, 2021, Little et al., 2020, Garcia-Garcia et al., 2017, Glise et al., 2002, Denef et al., 2000, Strutt and Mlodzik, 1997)

pka-c1^H2

(Cheng et al., 2012, Nakano et al., 2004)

pka^DCOH2

(Deshpande et al., 2001)

pka^h2

(Brook and Cohen, 1996)

Name Synonyms

Secondary FlyBase IDs

References (44)

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