FlyBase Allele Report: Dmel\dco[3]

General Information

Symbol

Dmel\dco³

Species

D. melanogaster

Name

FlyBase ID

FBal0032016

Feature type

allele

Associated gene

Dmel\dco

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Jia et al., 2005)

Mutagen

ethyl methanesulfonate

(Jursnich et al., 1990)

Progenitor genotype

Cytology

Description

The mutated residues R4 and E74 fall in the catalytic domain of the wild type protein. E74 lies in the region of the catalytic domain thought to face the protein substrates, not in the region involved in the catalytic activity. dco² and dco³ share a D401Q polymorphism.

(Zilian et al., 1999)

Amino acid replacement: R4C.

(Zilian et al., 1999)

Amino acid replacement: E74K.

(Zilian et al., 1999)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:31,057,632..31,057,632 [-]

Nucleotide change:

G31057632A

Amino acid change:

Reported amino acid change:

E74K

Comment:

One of two amino acid changes observed in dco³. Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Zilian et al., 1999)

point_mutation

3R:31,057,842..31,057,842 [-]

Nucleotide change:

C31057842T

Amino acid change:

Reported amino acid change:

R4C

Comment:

One of two amino acid changes observed in dco³. Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Zilian et al., 1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult external head

(Zilian et al., 1999)

eye

(Zilian et al., 1999)

imaginal disc

(Zilian et al., 1999)

imaginal disc | somatic clone

(Zilian et al., 1999)

ommatidium

(Zilian et al., 1999)

tarsal segment

(Zilian et al., 1999)

unguis | increased number (with Df(3R)PGX8)

(Zilian et al., 1999)

vibrissal bristle

(Zilian et al., 1999)

wing disc

(Dolezal et al., 2010)

wing disc, with dco^L39Q

(Dolezal et al., 2010)

wing disc (with dco^L39Q)

(Dolezal et al., 2010)

wing disc (with dco^le88)

(Jia et al., 2005)

wing disc | larval stage

(Cho et al., 2006)

wing pouch

(Dolezal et al., 2010)

wing pouch, with dco^L39Q

(Dolezal et al., 2010)

wing pouch (with dco^L39Q)

(Dolezal et al., 2010)

Detailed Description

Statement

Reference

dco³ mutant clones in the eye imaginal disc are overgrown compared to controls.

(Bosch et al., 2014)

The overall morphology of dco³ mutants is abnormal, with extensive rippling and loss of normal morphological features including the wing pouch.

dco³/dco^t.L39Q transheterozygotes are similar to dco³ mutants.

(Dolezal et al., 2010)

The rough eye phenotype observed in Fmr1^sev.PW flies is suppressed in a dco³/+ background.

(Cziko et al., 2009)

dco³ clones in the adult abdomen do not show planar cell polarity defects.

(Feng and Irvine, 2009)

dco³ clones in the wing imaginal disc are larger than their associated wild type sibling clones.

(Galletti et al., 2009)

dco³ mutant wing discs collected 36-48 hours after the L2-L3 molt are overgrown.

(Cho et al., 2006)

dco³/dco^le88 wing discs exhibit an overgrowth phenotype.

(Jia et al., 2005)

Homozygotes show lethality in larval or early pupal stages, with overgrown discs. Pharate adults generated with dco³/hypomorph combinations show morphogenetic aberrations including duplicated structures. dco³ in combination with dco² or dco¹⁸ : lethality in larval or early pupal stages, with overgrown discs. dco³ in combination with dco^le88, dco^i3-193, dco^S053813, dco^S139602, dco^S091510 and dco^P103 : show lethality during larval or early pupal stages with typical dco³ phenotype (swollen tarsi with or without head defects). Discs overgrown. dco³ in combination with dco^S144701 : adult viable with reduced eyes extra vibrissae and expanded wings. dco³ somatic clones in the discs show an autonomous overgrowth phenotype. Cell size is unaffected. Animals with many clones show delayed pupariation.

(Zilian et al., 1999)

Hyperplastic overgrowth mutant. Apico-lateral junctional complexes appear normal.

(Woods et al., 1997)

Hyperplastic imaginal disc overgrowth, affecting all discs. Discs implanted into wild type hosts for metamorphosis over grow and differentiate abnormal imaginal structures. dco¹⁸/dco³ mutant larvae grow at a normal rate until end of feeding period of last larval instar, when growth slows and then stops: they eventually reach double the weight of wild type pupariating larvae. The ecdysteroid secretory activity of dlg1 and dco ring glands in vitro is 1/6 that of wild type.

(Sehnal and Bryant, 1993)

Larval life prolonged by up to 14 days. Overgrown larvae show bloating and an increase in water content.

(Zitnan et al., 1993)

When raised at 25^oC the larval period of homozygotes and interallelic heterozygotes is greatly extended and they develop overgrown imaginal discs. Imaginal discs show gap-junctional communication defects: coupling is severely diminished. The single layer epithelium is maintained during overgrowth and they differentiate into the correct but abnormal adult structures.

(Jursnich et al., 1990)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

dco³ has abnormal size | somatic clone phenotype, suppressible by Scer\GAL4^unspecified/Fbxl7^UAS.Tag:FLAG

(Bosch et al., 2014)

dco³ has lethal phenotype, suppressible by Scer\GAL4^Tub.PU/wts^UAS.Tag:MYC

(Feng and Irvine, 2009)

dco³ has abnormal size phenotype, suppressible by Scer\GAL4^Tub.PU/wts^UAS.Tag:MYC

(Feng and Irvine, 2009)

dco³ has increased cell number | larval stage phenotype, suppressible by dachs¹

(Cho et al., 2006)

Enhancer of

Statement

Reference

dco[+]/dco³ is an enhancer of abnormal cell polarity phenotype of fz²⁰/fz¹⁹

(Strutt et al., 2006)

Phenotype Manifest In

Suppressed by

Statement

Reference

dco³ has wing disc | larval stage phenotype, suppressible by dachs¹

(Cho et al., 2006)

Enhancer of

Statement

Reference

dco[+]/dco³ is an enhancer of ommatidium phenotype of fz²⁰/fz¹⁹

(Strutt et al., 2006)

Suppressor of

Statement

Reference

dco[+]/dco³ is a suppressor of eye phenotype of Fmr1^sev.PW

(Cziko et al., 2009)

Other

Statement

Reference

Scer\GAL4^Tub.PU, dco³, wts^UAS.Tag:MYC has wing vein phenotype

(Feng and Irvine, 2009)

Additional Comments

Genetic Interactions

Statement

Reference

The increased size of dco³ clones is rescued by Fbxl7^{Scer\UAS.T:Zzzz\FLAG} expression.

(Bosch et al., 2014)

The lethality and overgrowth phenotypes of dco³ animals are rescued by Scer\GAL4^tub.PU-mediated overexpression of wts^{Scer\UAS.T:Hsap\MYC}, resulting in animals that, aside from some mild wing vein phenotypes, are indistinguishable from wild type animals over-expressing wts^{Scer\UAS.T:Hsap\MYC}.

(Feng and Irvine, 2009)

d¹ suppresses the wing disc overgrowth seen in dco³ mutant larvae.

(Cho et al., 2006)

The ommatidial polarity defects of fz¹⁹/fz²⁰ mutants are enhanced in fz¹⁹/fz²⁰, dco³/+ double mutants.

(Strutt et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially complements

dco^S144701

(Zilian et al., 1999)

Fails to complement

dco^P103

(Zilian et al., 1999)

dco^i3-193

(Zilian et al., 1999)

dco^S053813

(Zilian et al., 1999)

dco^S091510

(Zilian et al., 1999)

dco^S139602

(Zilian et al., 1999)

dco^le88

(Zilian et al., 1999)

dco²

(Zilian et al., 1999)

dco¹⁸

(Zilian et al., 1999)

Rescued by

dco³ is rescued by dco^+t13.1

(Zilian et al., 1999)

Comments

Assessment of complementation based on lethal aspect of phenotype.

(Zilian et al., 1999)

Images (0)

Mutant

Wild-type

Stocks (3)

Bloomington

44250

w^*; P{UAS-wts.MYC}2/CyO; P{neoFRT}82B dco³/TM6B, Tb¹

2455

st¹ Ki¹ cu¹ e¹ dco³/TM6B, Tb⁺

Kyoto

107341

st¹ Ki¹ cu¹ dco³/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

dco³

(Matakatsu et al., 2017, Kelly et al., 2016, Zhang et al., 2016, Bosch et al., 2014, Legent et al., 2012, Dolezal et al., 2010, Christensen et al., 2009.2.28, Cziko et al., 2009, Feng and Irvine, 2009, Galletti et al., 2009, Sopko et al., 2009, Feng et al., 2007, Cho et al., 2006, Strutt et al., 2006, Zhang et al., 2006, Jia et al., 2005)

Name Synonyms

Secondary FlyBase IDs

References (24)

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