FB2024_03 , released June 25, 2024
Allele: Dmel\dco18
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General Information
Symbol
Dmel\dco18
Species
D. melanogaster
Name
FlyBase ID
FBal0032014
Feature type
allele
Associated gene
Dmel\dco
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

hypomorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

hypomorphic allele - genetic evidence

(Zilian et al., 1999)
Mutagen
ethyl methanesulfonate
(Jursnich et al., 1990)
Progenitor genotype
Cytology
Description

The mutated residue S181 is strictly conserved in, and specific for, the CKI kinase family. S181 falls in the L-9D loop. This lesion may weaken or prevent the interaction with at least some of the dco substrates, without affecting its catalytic activity.

(Zilian et al., 1999)

Amino acid replacement: S181F.

(Zilian et al., 1999)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3R:31,057,310..31,057,310 [-]
Nucleotide change:

C31057310T

Amino acid change:

S181F | dco-PA; S181F | dco-PB; S181F | dco-PC; S181F | dco-PD; S181F | dco-PE

Reported amino acid change:

S181F

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Zilian et al., 1999)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      dco3 in combination with dco2 or dco18 : lethality in larval or early pupal stages, with overgrown discs. dco2 in combination with dco18 : show lethality during larval or early pupal stages, discless phenotype. dco18 in combination with dcole88, dcoi3-193, or dcoS053813 : show lethality during larval stages. dco18 in combination with dcoS139602 or dcoS091510 : show lethality during larval or early pupal stages with a discless phenotype. dco18 in combination with dcoP103 : show lethality during larval or early pupal stages with abnormal discs with granular zone and abnormal folding. dco18 in combination with dcoS144701 :adult viable with narrow wings, reduced eyes, disordered facets and minor wing vein defects.

      (Zilian et al., 1999)

      Hyperplastic imaginal disc overgrowth, affecting all discs. Discs implanted into wild type hosts for metamorphosis over grow and differentiate abnormal imaginal structures. dco18/dco3 mutant larvae grow at a normal rate until end of feeding period of last larval instar, when growth slows and then stops: they eventually reach double the weight of wild type pupariating larvae. The ecdysteroid secretory activity of dlg1 and dco ring glands in vitro is 1/6 that of wild type.

      (Sehnal and Bryant, 1993)

      Larval life prolonged by up to 14 days. Overgrown larvae show bloating and an increase in water content.

      (Zitnan et al., 1993)

      Hemizygous embryos are non-viable. Some die in the egg case, and have variable head abnormalities. The head is absent and there is a hole in the dorsal larval cuticle in the most severe cases. Homozygous embryos show polyphasic lethality, with most larvae dying around the second-third larval instar transition. A few larvae survive to the third instar, having small imaginal discs with abnormal folds.

      (Szabad et al., 1991)

      When raised at 25oC the larval period of dco18 flies in heteroallelic combinations is greatly extended and they develop overgrown imaginal discs. Imaginal discs show gap-junctional communication defects: coupling is severely diminished. The single layer epithelium is maintained during overgrowth and they differentiate into the correct but abnormal adult structures. Larvae have double mouth hooks, two sets of filzkorper and setal belts and defects around the labrum and dorsal bridge region of the head skeleton.

      (Jursnich et al., 1990)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer

      Separable from: an undefined, linked embryonic lethal mutation.

      (Zilian et al., 1999)
      Comments
      Comments

      Allelic series in order of decreasing strength: dcole88 > dco18 > dco2.

      (Zilian et al., 1999)

      Pole cell transplantation shows that this allele has no effect on germ-line development.

      (Szabad et al., 1991)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      dco18
      Name Synonyms
      Secondary FlyBase IDs
        References (5)