FlyBase Allele Report: Dmel\dco[le88]

General Information

Symbol

Dmel\dco^le88

Species

D. melanogaster

Name

FlyBase ID

FBal0032019

Feature type

allele

Associated gene

Dmel\dco

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Jia et al., 2005, Zilian et al., 1999)

Mutagen

ethyl methanesulfonate

(Jursnich et al., 1990)

Progenitor genotype

Cytology

Description

Deletion of 369bp in the dco transcription unit, at the 5' end of the ORF, removing the last intron/exon boundary and ends after the T of the ATG initiation codon.

(Zilian et al., 1999)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3R:31,057,850..31,058,218 [-]

Comment:

Deletion of 369bp that removes the last intron/exon boundary and ends after the T of the ATG initiation codon.

(Zilian et al., 1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell death | cell autonomous | recessive | somatic clone

(Zilian et al., 1999)

abnormal developmental rate, with Df(3R)A177der20, dco^L39Q, dco^S101R

(Dolezal et al., 2010)

abnormal developmental rate (with Df(3R)A177der20), with dco^L39Q

(Dolezal et al., 2010)

lethal | cell autonomous | recessive | somatic clone

(Zilian et al., 1999)

lethal | larval stage | recessive

(Zilian et al., 1999)

lethal | recessive

(Szabad et al., 1991)

Phenotype Manifest In

adult external head

(Zilian et al., 1999)

crossvein, with Df(3R)A177der20, dco^L39Q, dco^S101R

(Dolezal et al., 2010)

crossvein (with Df(3R)A177der20), with dco^L39Q

(Dolezal et al., 2010)

egg | germline clone

(Szabad et al., 1991)

eye

(Zilian et al., 1999)

imaginal disc

(Zilian et al., 1999)

imaginal disc (with Df(3R)A177der20), with dco^L39Q

(Dolezal et al., 2010)

imaginal disc | somatic clone

(Zilian et al., 1999)

larva (with Df(3R)A177der20), with dco^L39Q

(Dolezal et al., 2010)

ommatidium

(Zilian et al., 1999)

tarsal segment

(Zilian et al., 1999)

wing (with Df(3R)A177der20), with dco^L39Q

(Dolezal et al., 2010)

wing disc, with Df(3R)A177der20, dco^L39Q, dco^S101R

(Dolezal et al., 2010)

wing disc (with dco³)

(Jia et al., 2005)

wing pouch (with Df(3R)A177der20), with dco^L39Q

(Dolezal et al., 2010)

wing vein

(Zilian et al., 1999)

Detailed Description

Statement

Reference

The presence of dco^t.L39Q in a dco^le88/Df(3R)A177der20 background results in an extended larval development (10 days compared to 5 days for wild-type larvae) associated with a striking overgrowth phenotype in larval imaginal discs. Whereas the wild-type disc stop growing when they reach the appropriate size, the mutant discs continue growing during the extended larval period and before pupariation they reach about three times the normal final size. Although the mutant larvae eventually pupate they do not proceed through metamorphosis, and the pupae die without any signs of adult development. The overall morphology of these mutants is abnormal, with extensive rippling and loss of normal morphological features including the wing pouch. These mutant discs retain their monolayered epithelial structure showing that they exhibit a hyperplastic overgrowth phenotype.

The presence of dco^t.L39Q and dco^t.S101R in a dco^le88/Df(3R)A177der20 background results in an extended larval development period. The discs do not grow larger than normal, but the tissue seems to disintegrate in places, indicating a loss of tissue integrity.

The presence of dco^t.L39Q and dco^t.S101R in a dco^le88/Df(3R)A177der20 background results in a very mild dominant effect on a crossvein in the adult wing, with less than 15% penetrance.

(Dolezal et al., 2010)

dco³/dco^le88 wing discs exhibit an overgrowth phenotype.

(Jia et al., 2005)

Homozygotes show larval lethality with a discless phenotype. dco³ in combination with dco^le88, dco^i3-193, dco^S053813, dco^S139602, dco^S091510 and dco^P103 : show lethality during larval or early pupal stages with typical dco³ phenotype (swollen tarsi with or without head defects). Discs overgrown. dco² in combination with dco^le88 : show lethality during larval or early pupal stages. dco¹⁸ in combination with dco^le88, dco^i3-193, or dco^S053813 : show lethality during larval stages. dco^le88 in combination with dco^i3-193 or dco^S053813 : show lethality during larval stages with a discless phenotype. dco^le88 in combination with dco^S139602 : show lethality during larval or early pupal stages with a discless phenotype. dco^le88 in combination with dco^S091510 : show lethality during larval or early pupal stages with a small discs phenotype. dco^le88 in combination with dco^P103 : show lethality during late pupal stage with abnormal discs with granular zone and abnormal folding. dco^le88 in combination with dco^S144701 : adult viable with reduced eyes, disordered facets and minor wing vein defects. In clones induced in the imaginal discs, homozygous dco^le88 cells are growth inhibited and fail to survive. This growth inhibition and apoptosis is cell autonomous.

(Zilian et al., 1999)

No vitellogenic egg primordia develop in chimeric females containing homozygous dco^le88 germ cells.

(Szabad et al., 1991)

Homozygotes are smaller than wild type at 4-5 days after egg laying. Larvae have correctly positioned imaginal discs. When homozygous or in heteroallelic combinations the imaginal discs degenerate producing a discless phenotype.

(Jursnich et al., 1990)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

BacA\p35^UAS.cHa, Scer\GAL4^en-e16E, dco^le88 has decreased cell number | somatic clone | larval stage phenotype

(Guan et al., 2007)

Phenotype Manifest In

Enhanced by

Statement

Reference

Df(3R)A177der20/dco^le88, dco^L39Q has crossvein phenotype, enhanceable by wts^P2

(Dolezal et al., 2010)

Df(3R)A177der20/dco^le88, dco^L39Q has wing phenotype, enhanceable by wts^P2

(Dolezal et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

dco^le88 mutant clones generated in an Scer\GAL4^en-e16E/BacA\p35^Scer\UAS.cHa background are consistently smaller than twin spots outside this background. The dco^le88 clones are on average 4 times smaller than the twin spots in the posterior compartment. Because the twins and clones originate from the two daughter cells of a single cell that underwent mitotic recombination, they grow for the same period of time in a similar location. Therefore the approimxate 1:4 ratio of clone/twin size in dco^le88 mutants suggests a defectin the rate of growth/proliferation.

(Guan et al., 2007)

Complementation and Rescue Data

Partially complements

dco^S144701

(Zilian et al., 1999)

Fails to complement

dco^P103

(Zilian et al., 1999)

dco^i3-193

(Zilian et al., 1999)

dco^S053813

(Zilian et al., 1999)

dco^S091510

(Zilian et al., 1999)

dco^S139602

(Zilian et al., 1999)

dco³

(Zilian et al., 1999)

dco²

(Zilian et al., 1999)

dco¹⁸

(Zilian et al., 1999)

Rescued by

dco^le88 is rescued by dco^+t13.1

(Zilian et al., 1999)

Comments

Assessment of complementation based on lethal aspect of phenotype.

(Zilian et al., 1999)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Allelic series in order of decreasing strength: dco^le88 > dco¹⁸ > dco².

(Zilian et al., 1999)

Pole cell transplantation shows that the dco^le88 mutation interferes with germ-line development.

(Szabad et al., 1991)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

dco^le88

(Fulford et al., 2019, Matakatsu et al., 2017, Bosch et al., 2014, Dolezal et al., 2010, Guan et al., 2007, Zhang et al., 2006, Jia et al., 2005)

Name Synonyms

Secondary FlyBase IDs

References (10)

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