FlyBase Allele Report: Dmel\shf[2]

General Information

Symbol

Dmel\shf²

Species

D. melanogaster

Name

FlyBase ID

FBal0015605

Feature type

allele

Associated gene

Dmel\shf

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

X ray

Nature of the Allele

Allele class

Mutagen

X ray

(Lindsley and Zimm, 1992)

Progenitor genotype

Cytology

Description

Amino acid replacement: C374S.

(Glise et al., 2005, Gorfinkiel et al., 2005)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:6,772,014..6,772,014 [-]

Nucleotide change:

T6772014A

Amino acid change:

C374S | shf-PA; C378S | shf-PB; C357S | shf-PC; C361S | shf-PD

Reported amino acid change:

C374S

Comment:

T to A nucleotide change at the first position or a G to C change at the second position of the Cys codon leads to a Ser (exact site of mutation unspecified). The mutation has been annotated at the first position of the codon.

(Glise et al., 2005, Gorfinkiel et al., 2005)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased body size

(Gorfinkiel et al., 2005)

fertile

(Lindsley and Zimm, 1992)

partially lethal - majority live

(Gorfinkiel et al., 2005)

semi-sterile

(Gorfinkiel et al., 2005)

viable

(Glise et al., 2005, Lindsley and Zimm, 1992)

Phenotype Manifest In

Detailed Description

Statement

Reference

In shf² mutant adult wings the spacing between the third and fourth longitudinal vein is greatly reduced.

(Avanesov et al., 2012)

shf^EY03173 is homozygous viable with a typical shf wing phenotype - including reduced distance between L3 and L4.

(Glise et al., 2005)

shf² homozygotes are semi-viable with a reduced fertility and a small body. Wing veins L3 and L4 are closer together than in wild-type wings and the anterior cross-vein is reduced or absent. The posterior scutellar bristles are also absent/

(Gorfinkiel et al., 2005)

Veins closer together than in wild type. L3 and L4 tend to fuse near anterior crossvein; anterior crossvein shortened, knotted, or absent. Phenotypic effect visible in prepupal wing bud, the two longitudinal veins diverging at a smaller-than-normal angle. Eyes sometimes slightly rough. Scutellar bristles often absent. Scutellum short. Wings narrow and often warped downward. RK2.

(Lindsley and Zimm, 1992)

Deletions and duplications of denticle belts. Narrow wings with short connecting extra veins between LIII and LIV.

(Diaz-Benjumea and Garcia-Bellido, 1990)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Drer\wif1^UAS.cAa, Scer\GAL4^nub-AC-62, shf² has lethal | pupal stage phenotype

(Avanesov et al., 2012)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

shf² has 1st posterior cell phenotype, non-suppressible by Hsap\WIF1^UAS.cGa/Scer\GAL4^unspecified

(Gorfinkiel et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Expression of Drer\wif1^Scer\UAS.cAa under the control of Scer\GAL4^nub-AC-62 in a shf² mutant background is lethal at the pupal stage.

(Avanesov et al., 2012)

Complementation and Rescue Data

Rescued by

shf² is rescued by shf^UAS.cGb/Scer\GAL4^Bx-MS1096

(Glise et al., 2005)

shf² is rescued by Scer\GAL4^en-e16E/shf^UAS.cGb

(Glise et al., 2005)

shf² is rescued by shf^UAS.cGb/Scer\GAL4^dpp.blk1

(Glise et al., 2005)

shf² is rescued by Scer\GAL4^ptc-559.1/shf^UAS.cGb

(Glise et al., 2005)

shf² is rescued by shf^UAS.cGb/Scer\GAL4^ap-md544

(Glise et al., 2005)

shf² is rescued by Scer\GAL4^hh-Gal4/shf^UAS.cGa

(Gorfinkiel et al., 2005)

Not rescued by

shf² is not rescued by Scer\GAL4^en-e16E/shf^δWIF.UAS

(Avanesov et al., 2012)

Comments

Expression of shf^{δWIF.Scer\UAS} under the control of Scer\GAL4^en-e16E is unable to rescue the wing vein phenotypes seen in shf²/Y mutants.

(Avanesov et al., 2012)

shf^Scer\UAS.cGa; Scer\GAL4^hh-Gal4 rescues the wing phenotype due to shf²/shf².

(Gorfinkiel et al., 2005)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

112

shf²

Notes on Origin

Discoverer

Oliver, 29th Oct. 1929.

(Lindsley and Zimm, 1992)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

D-Wif

(Guerrero et al., 2004)

shf²

(Benner et al., 2019, Avanesov and Blair, 2013, Bilioni et al., 2013, Avanesov et al., 2012, Sánchez-Hernández et al., 2012, Christensen and Cook, 2007.5.8, Christensen and Cook, 2007.5.8, Christensen and Cook, 2007.5.8, Christensen and Cook, 2007.5.8, Christensen and Cook, 2007.10.29)

Name Synonyms

Secondary FlyBase IDs

References (19)

Report Sections

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