Date: Tue, 08 May 2007  08:45:51  -0400
To: flybase-updates@XXXX
From: Kevin Cook <kcook@XXXX>
Subject: Isolation and characterization of Df(1)BSC276
Cc: mdealXXXX, Stacey Christensen <sjchristXXXX>, kaufman@XXXX
Isolation and characterization of Df(1)BSC276
Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(1)BSC276 was isolated as a FLP recombinase-induced recombination 
event involving P{XP}d07849 and PBac{WH}CG14435[f07374]. The deletion 
was isolated as a chromosome lacking miniwhite markers in progeny of 
w[1118] P{XP}d07849/w[1118] PBac{WH}CG14435[f07374]; MKRS, 
P{hsFLP}86E/+ females crossed to Binsinscy/Y males. These females 
were heat shocked as larvae as described in Parks et al., Nature 
Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in 
preceding and succeeding generations maintained the original genetic 
background of the Exelixis insertion stocks (Thibault et al., Nature 
Genetics 36: 283-287, 2004; FBrf0175002). The recombination event 
generated the genetic element P+PBac{XP5.WH5}BSC276 from the segment 
of P{XP}d07849 to the left of its FRT site and the segment of 
PBac{WH}CG14435[f07374] to the right of its FRT site. Its presence 
was verified using the PCR methods and primers described in Parks et 
al.  The cytological breakpoints of Df(1)276 predicted from the 
Release 5 genomic coordinates of the transposable element insertions 
sites are 6C12;6D4. It failed to complement shf[2].
__________________________________________________________
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology           http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX