Date: Tue, 08 May 2007 08:45:00 -0400 To: flybase-updates@XXXX From: Kevin Cook <kcook@XXXX> Subject: Isolation and characterization of Df(1)BSC297 Cc: mdealXXXX, Stacey Christensen <sjchristXXXX>, kaufman@XXXX Isolation and characterization of Df(1)BSC297 Stacey Christensen and Kevin Cook Bloomington Stock Center Indiana University Df(1)BSC297 was isolated as a FLP recombinase-induced recombination event involving P{XP}d07849 and PBac{WH}CG3044[f02328]. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w[1118] P{XP}d07849/w[1118] PBac{WH}CG3044[f02328]; MKRS, P{hsFLP}86E/+ females crossed to Binsinscy/Y males. These females were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC297 from the segment of P{XP}d07849 to the left of its FRT site and the segment of PBac{WH}CG3044[f02328] to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(1)BSC297 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 6C12;6D6. It failed to complement shf[2]. __________________________________________________________ Kevin Cook, Ph.D. Bloomington Drosophila Stock Center Department of Biology http://flystocks.bio.indiana.edu Jordan Hall 142 Indiana University 812-856-1213 1001 E. Third St. 812-855-2577 (fax) Bloomington, IN 47405-7005 kcook@XXXX