FlyBase Allele Report: Dmel\rt[P]

General Information

Symbol

Dmel\rt^P

Species

D. melanogaster

Name

FlyBase ID

FBal0014813

Feature type

allele

Associated gene

Dmel\rt

Associated Insertion(s)

P{hsneo}rt^P

Carried in Construct

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(BDGP Project Members, 1994-1999, Cooley et al., 1988)

Progenitor genotype

Associated Insertion(s)

P{hsneo}rt^P

Cytology

Description

P{hsneo} is inserted into the first exon of rt.

(Martin-Blanco and Garcia-Bellido, 1996)

P-element insertion within the rt locus.

(Cooley et al., 1988)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Walker-Warburg syndrome

CEA

(Wairkar et al., 2008)

model of congenital muscular dystrophy-dystroglycanopathy type A1

CEA

(Wairkar et al., 2008)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of Walker-Warburg syndrome

is exacerbated by Dg^e01554

(Wairkar et al., 2008)

model of congenital muscular dystrophy-dystroglycanopathy type A1

is exacerbated by Dg^e01554

(Wairkar et al., 2008)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neurophysiology (with Df(3L)ED4470)

(Wairkar et al., 2008)

female semi-fertile

(Cooley et al., 1988)

female sterile | recessive

(Spradling et al., 1999)

male semi-fertile

(Cooley et al., 1988)

visible | recessive

(Cooley et al., 1988)

Phenotype Manifest In

adult abdomen

(Lyalin et al., 2006, Cooley et al., 1988)

adult abdomen (with rt²)

(Lyalin et al., 2006)

adult abdomen (with rt^EP571)

(Lyalin et al., 2006)

adult cuticle

(Martin-Blanco and Garcia-Bellido, 1996)

embryonic/larval muscle system | third instar larval stage (with rt²)

(Haines et al., 2007)

muscle attachment site | third instar larval stage (with rt²)

(Haines et al., 2007)

sarcomere | third instar larval stage (with rt²)

(Haines et al., 2007)

Detailed Description

Statement

Reference

Recordings from larval neuromuscular junctions (muscle 6 of A3 or 4) of rt^P/Df(3L)ED4470 animals show a dramatic decrease in evoked synaptic transmission. However, the size and frequency of spontaneous mini excitatory junction potentials is normal. Based on this, estimated quantal content is reduced about 3 fold. This phenotype is unaffected by the suppression of action potentials with 1 micro-molar TTX. Short term facilitation is enhanced in these mutants, suggesting that decreased quantal content is due to decreased probability of vesicle release in response to stimulus.

(Wairkar et al., 2008)

Muscle attachment defects are observed in rt²/rt^P mutant third instar larvae. Sarcomeres are larger than in control animals.

(Haines et al., 2007)

rt^P, rt^P/rt^EP571 and rt^P/rt² flies have rotated abdomens.

(Lyalin et al., 2006)

The bodies of first instar larvae are rotated around the long axis, forcing the animals to move in a clockwise circle as they crawl. The larval cuticle presents a perfect segmental alignment of landmarks but the adult abdominal cuticle segments appear staggered relative to each other.

(Martin-Blanco and Garcia-Bellido, 1996)

Homozygous males and females have reduced fertility and show abdominal rotation.

(Cooley et al., 1988)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Dg[+]/Dg^e01554, rt^P has abnormal neurophysiology phenotype

(Wairkar et al., 2008)

Dg^e01554, rt[+]/rt^P has abnormal neurophysiology phenotype

(Wairkar et al., 2008)

Dg³²³, rt[+]/rt^P has partially lethal - majority die | dominant phenotype

(Haines et al., 2007)

Phenotype Manifest In

Enhanced by

Statement

Reference

rt²/rt^P has embryonic/larval muscle system | third instar larval stage phenotype, enhanceable by tw¹

(Haines et al., 2007)

rt²/rt^P has muscle attachment site | third instar larval stage phenotype, enhanceable by tw¹

(Haines et al., 2007)

rt²/rt^P has sarcomere | third instar larval stage phenotype, enhanceable by tw¹

(Haines et al., 2007)

NOT Enhanced by

Statement

Reference

rt²/rt^P has adult abdomen phenotype, non-enhanceable by Scer\GAL4^Act5C.PI/tw^UAS.Tag:HA

(Lyalin et al., 2006)

rt²/rt^P has adult abdomen phenotype, non-enhanceable by tw^RNAi.UAS/Scer\GAL4^Act5C.PI

(Lyalin et al., 2006)

rt^P/rt^EP571 has adult abdomen phenotype, non-enhanceable by Dp(1;Y)y²sc/+

(Lyalin et al., 2006)

Suppressed by

Statement

Reference

rt^P has adult abdomen phenotype, suppressible by tw[+]/tw¹

(Lyalin et al., 2006)

rt^P has adult abdomen phenotype, suppressible by tw^RNAi.UAS/Scer\GAL4^Act5C.PI

(Lyalin et al., 2006)

rt^P has adult abdomen phenotype, suppressible by tw^RNAi.UAS/Scer\GAL4^αTub84B.PL

(Lyalin et al., 2006)

rt^P/rt^EP571 has adult abdomen phenotype, suppressible by tw[+]/tw¹

(Lyalin et al., 2006)

Other

Statement

Reference

Dg³²³, rt[+]/rt^P has embryonic/larval muscle system | early third instar larval stage phenotype

(Haines et al., 2007)

Dg³²³, rt[+]/rt^P has muscle attachment site | early third instar larval stage phenotype

(Haines et al., 2007)

Dg³²³, rt[+]/rt^P has sarcomere | early third instar larval stage phenotype

(Haines et al., 2007)

Scer\GAL4^Act5C.PHb, rt^P, tw^RNAi.UAS.cIa has adult abdomen phenotype

(Ichimiya et al., 2004)

Scer\GAL4^Act5C.PHb, rt[+]/rt^P, tw^RNAi.UAS.cIa has adult abdomen phenotype

(Ichimiya et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Evoked excitatory junction potentials are normal at the neuromuscular junctions of rt^P/+ or Dg^e01554/+ larvae, but siginficantly reduced compared to wild-type in rt^P/+; Dg^e01554/+ larvae. However, the size and frequency of spontaneous mini excitatory junction potentials is normal in transheterozygotes.

(Wairkar et al., 2008)

Dg³²³/+;rt^P/+ third instar larvae show muscle attachment defects and large sarcomeres.

A greater number of muscle attachment defects are observed in tw¹; rt²/rt^P mutant third instar larvae compared to rt²/rt^P mutants. Sarcomeres are larger than in wildtype animals.

(Haines et al., 2007)

The rotated abdomen phenotype of rt^P/rt^EP571 flies is not enhanced by the presence of the Dp(1;Y)y²sc duplication. Likewise, expression of the tw^{Scer\UAS.T:Ivir\HA1} transgene, under the control of Scer\GAL4^Act5C.PI, does not enhance the abdominal phenotype of rt^P/rt² mutants. This phenotype is also not affected by tw RNAi, achieved by expression of tw^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Act5C.PI. However, expression of tw^{dsRNA.Scer\UAS}, driven by Scer\GAL4^Act5C.PI or Scer\GAL4^αTub84B.PL, reduces the rotated abdomen phenotype of rt^P/+ flies.

(Lyalin et al., 2006)

At 18^oC, flies expressing Pomt2^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Act5C.PHb in a rt^P/+ background show a clear twisted abdomen phenotype, in contrast to either single mutant (rt^P/+ or flies expressing Pomt2^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Act5C.PHb at 18^oC in a wild-type background).

(Ichimiya et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Df(3L)ED4470/rt^P is rescued by rt^UAS.cWa/Scer\GAL4^Act5C.PU

(Wairkar et al., 2008)

Partially rescued by

Df(3L)ED4470/rt^P is partially rescued by rt^UAS.cWa/Scer\GAL4^C142

(Wairkar et al., 2008)

Comments

Evoked excitatory junction potentials are normal at the neuromuscular junctions of rt^P/+ or Dg^e01554/+ larvae, but significantly reduced compared to wild-type in rt^P/+; Dg^e01554/+ larvae. However, the size and frequency of spontaneous mini excitatory junction potentials is normal in transheterozygotes.

(Wairkar et al., 2008)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

10060

mwh¹ P{hsneo}rt^P red¹ e¹/TM3, Sb¹ Ser¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

dPOMT1

(Wairkar et al., 2008)

fs(3)neo1

(Spradling et al., 1999)

fs(3)neo1¹

rt^P60

(Martin-Blanco and Garcia-Bellido, 1996)

rt^P

(Monagas-Valentin et al., 2023, Nakamura et al., 2010, Christensen et al., 2008.9.3, Christensen et al., 2008.12.28, Wairkar et al., 2008, Lyalin et al., 2006)

rt^neo1

(Cooley et al., 1988)

Name Synonyms

Secondary FlyBase IDs

References (14)

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