FlyBase Allele Report: Dmel\tw[RNAi.UAS.cIa]

General Information

Symbol

Dmel\tw^RNAi.UAS.cIa

Species

D. melanogaster

Name

FlyBase ID

FBal0159998

Feature type

allele

Associated gene

Dmel\tw

Associated Insertion(s)

Carried in Construct

P{UAS-tw.RNAi.I}

Key Links

Transgenic product class

RNAi_reagent

(Ichimiya et al., 2004)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ichimiya et al., 2004)

Mutagen

in vitro construct

(Ichimiya et al., 2004)

Progenitor genotype

Carried in construct

P{UAS-tw.RNAi.I}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat of nucleotides 792-1289 of the coding sequence of tw.

(Ichimiya et al., 2004)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Walker-Warburg syndrome

CEA

(Ueyama et al., 2010)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

partially lethal | heat sensitive, with Scer\GAL4^Act5C.PHb

(Ichimiya et al., 2004)

Phenotype Manifest In

adult abdomen, with Scer\GAL4^Act5C.PHb

(Ichimiya et al., 2004)

Detailed Description

Statement

Reference

Deficient or thin muscles are sometimes seen in the abdominal segments of larvae which are expressing tw^{dsRNA.Scer\UAS.cIa} under the control of Scer\GAL4^Act5C.PU.

(Ueyama et al., 2010)

Flies expressing Pomt2^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Act5C.PHb have a viability of 63% at 28^oC. Adult escapers expressing Pomt2^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Act5C.PHb show a clockwise twisted abdomen phenotype (the phenotype is seen in both males and females). Flies expressing Pomt2^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Act5C.PHb at 18^oC have no aberrant phenotype.

(Ichimiya et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

tw^RNAi.UAS.cIa, Scer\GAL4^en-e16E is an enhancer of visible phenotype of Dg^RNAi.UAS.cUa, Scer\GAL4^en-e16E

(Ueyama et al., 2010)

Other

Statement

Reference

Scer\GAL4^Act5C.PHb, rt^RNAi.UAS, tw^RNAi.UAS.cIa has lethal phenotype

(Ichimiya et al., 2004)

Phenotype Manifest In

Enhancer of

Statement

Reference

tw^RNAi.UAS.cIa, Scer\GAL4^en-e16E is an enhancer of wing phenotype of Dg^RNAi.UAS.cUa, Scer\GAL4^en-e16E

(Ueyama et al., 2010)

Other

Statement

Reference

Scer\GAL4^Act5C.PHb, rt^P, tw^RNAi.UAS.cIa has adult abdomen phenotype

(Ichimiya et al., 2004)

Scer\GAL4^Act5C.PHb, rt[+]/rt^P, tw^RNAi.UAS.cIa has adult abdomen phenotype

(Ichimiya et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

The penetrance of the blistered wing phenotype caused by expression of Dg^{dsRNA.Scer\UAS.cUa} under the control of Scer\GAL4^en-e16E is significantly enhanced by co-expression of tw^{dsRNA.Scer\UAS.cIa} at 28[o]C.

(Ueyama et al., 2010)

At 18^oC, flies expressing Pomt2^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Act5C.PHb in a rt^P/+ background show a clear twisted abdomen phenotype, in contrast to either single mutant (rt^P/+ or flies expressing Pomt2^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Act5C.PHb at 18^oC in a wild-type background). Animals expressing both Pomt2^{dsRNA.Scer\UAS} and rt^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Act5C.PHb show complete lethality.

(Ichimiya et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Pomt2^{dsRNA.Scer\UAS}

tw^RNAi.UAS.cIa

tw^{dsRNA.Scer\UAS.2}

tw^{dsRNA.Scer\UAS.cIa}

tw^{dsRNA.UAS.cIa}

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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