FlyBase Allele Report: Dmel\rt[2]

General Information

Symbol

Dmel\rt²

Species

D. melanogaster

Name

FlyBase ID

FBal0014814

Feature type

allele

Associated gene

Dmel\rt

Associated Insertion(s)

?{}rt²

Carried in Construct

Key Links

Allele class

Mutagen

spontaneous

Nature of the Allele

Allele class

Mutagen

spontaneous

(Martin-Blanco and Garcia-Bellido, 1996)

Progenitor genotype

Associated Insertion(s)

?{}rt²

Cytology

Description

Insertion of a transposable element into the same genomic region as the insertion in rt¹.

(Martin-Blanco and Garcia-Bellido, 1996)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of muscular dystrophy-dystroglycanopathy

CEA with rt^EP571

(Monagas-Valentin et al., 2023)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Deficient or thin muscles are sometimes seen in the abdominal segments of homozygous larvae.

(Ueyama et al., 2010)

Muscle attachment defects are observed in rt² mutant third instar larvae. Sarcomeres are larger than in wildtype animals.

Muscle attachment defects are observed in rt²/rt^P mutant third instar larvae. Sarcomeres are larger than in wildtype animals.

(Haines et al., 2007)

rt^P/rt² flies have rotated abdomens.

(Lyalin et al., 2006)

The bodies of first instar larvae are rotated around the long axis, forcing the animals to move in a clockwise circle as they crawl. The larval cuticle presents a perfect segmental alignment of landmarks but the adult abdominal cuticle segments appear staggered relative to each other. Embryos show a variable but fully penetrant mutant phenotype. Common phenotypes include the lack of some muscles or the presence of very elongated thin ones. Most affected muscles are the lateral muscles 4, 5, 8, 23 and 24. Dorsal muscles are less commonly missing.

(Martin-Blanco and Garcia-Bellido, 1996)

Abdomen twisted, as is rt. Viability erratic, usually about 50% wild type. Male fertile; female not tested. RK2.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

rt² is a non-enhancer of lethal | early third instar larval stage phenotype of Dg³²³

(Haines et al., 2007)

Phenotype Manifest In

Enhanced by

Statement

Reference

rt² has muscle attachment site | third instar larval stage phenotype, enhanceable by tw¹

(Haines et al., 2007)

rt²/rt^P has embryonic/larval muscle system | third instar larval stage phenotype, enhanceable by tw¹

(Haines et al., 2007)

rt²/rt^P has muscle attachment site | third instar larval stage phenotype, enhanceable by tw¹

(Haines et al., 2007)

rt²/rt^P has sarcomere | third instar larval stage phenotype, enhanceable by tw¹

(Haines et al., 2007)

rt² has embryonic/larval muscle system | third instar larval stage phenotype, enhanceable by tw¹

(Haines et al., 2007)

NOT Enhanced by

Statement

Reference

rt²/rt^P has adult abdomen phenotype, non-enhanceable by Scer\GAL4^Act5C.PI/tw^UAS.Tag:HA

(Lyalin et al., 2006)

rt²/rt^P has adult abdomen phenotype, non-enhanceable by tw^RNAi.UAS/Scer\GAL4^Act5C.PI

(Lyalin et al., 2006)

NOT Enhancer of

Statement

Reference

rt² is a non-enhancer of embryonic/larval muscle system | early third instar larval stage phenotype of Dg³²³

(Haines et al., 2007)

rt² is a non-enhancer of muscle attachment site | early third instar larval stage phenotype of Dg³²³

(Haines et al., 2007)

rt² is a non-enhancer of sarcomere | early third instar larval stage phenotype of Dg³²³

(Haines et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

Double homozygous Dg³²³;rt² larvae show changes in sarcomere size and muscle attachment defects, identical to those seen Dg³²³ homozygous animals.

A greater number of muscle attachment defects are observed in tw¹; rt² mutant third instar larvae compared to rt² mutants. Sarcomeres are larger than in wildtype animals.

A greater number of muscle attachment defects are observed in tw¹; rt²/rt^P mutant third instar larvae compared to rt²/rt^P mutants. Sarcomeres are larger than in wildtype animals.

(Haines et al., 2007)

Expression of the tw^{Scer\UAS.T:Ivir\HA1} transgene, under the control of Scer\GAL4^Act5C.PI, does not enhance the abdominal phenotype of rt^P/rt² mutants. This phenotype is also not affected by tw RNAi, achieved by expression of tw^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Act5C.PI.

(Lyalin et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

594

se¹ rt² Diap1^th-1/In(3L)P, Me¹

Kyoto

105997

se¹ rt² Diap1^th-1/In(3L)P, Me¹

Notes on Origin

Discoverer

Bridges, 14th Dec. 1925.

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

rt²

(Monagas-Valentin et al., 2023, Nakamura et al., 2010, Ueyama et al., 2010, Christensen et al., 2008.9.3, Christensen et al., 2008.12.28, Lyalin et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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