FB2024_03 , released June 25, 2024
Allele: Dmel\twRNAi.UAS
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General Information
Symbol
Dmel\twRNAi.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0195423
Feature type
allele
Associated gene
Dmel\tw
Associated Insertion(s)
Carried in Construct
P{UAS-tw.RNAi}
Key Links
Transgenic product class
RNAi_reagent
(Lyalin et al., 2006)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Lyalin et al., 2006)
Mutagen
in vitro construct
(Lyalin et al., 2006)
Progenitor genotype
Carried in construct
P{UAS-tw.RNAi}
Cytology
Description

Expression of a ~700bp inverted repeat of tw, which encodes exon 6, separated by the intron that follows this exon, is driven by UASt sequences.

(Lyalin et al., 2006)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
tw
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      NOT Enhancer of
      Statement
      Reference

      twRNAi.UAS/Scer\GAL4Act5C.PI is a non-enhancer of adult abdomen phenotype of rt2/rtP

      (Lyalin et al., 2006)
      Suppressor of
      Statement
      Reference

      twRNAi.UAS/Scer\GAL4Act5C.PI is a suppressor of adult abdomen phenotype of rtP

      (Lyalin et al., 2006)

      twRNAi.UAS/Scer\GAL4αTub84B.PL is a suppressor of adult abdomen phenotype of rtP

      (Lyalin et al., 2006)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The rotated abdomen phenotype of rtP/rt2 flies is not affected by tw RNAi. The RNAi is achieved by expression of twdsRNA.Scer\UAS under the control of Scer\GAL4Act5C.PI. However, expression of twdsRNA.Scer\UAS, driven by Scer\GAL4Act5C.PI ot Scer\GAL4αTub84B.PL, reduces the rotated abdomen phenotype of rtP/+ flies.

      (Lyalin et al., 2006)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Transfected into flies to study the phenotypic consequences of dsRNA interference (RNAi) of the tw gene.

      (Lyalin et al., 2006)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      twRNAi.UAS
      twdsRNA.Scer\UAS
      twdsRNA.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (2)