From: 	Kevin Cook <kcook@XXXX>
To: 	FlyBase-Cambridge <flybase-cambridgeXXXX>, Kim Cook <ruacookXXXX>, Stacey Christensen <sjchristXXXX>, kaufmanXXXX
Subject: 	Isolation and characterization of Df(3L)BSC577
Date: 	Wed, 03 Sep 2008  11:58:38  -0400  ( 16:58  BST)
Isolation and characterization of Df(3L)BSC577
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC577 was isolated as a FLP recombinase-induced recombination 
event involving P{XP}Mob2[d11543] and PBac{RB}CG32085[e02816]. The 
deletion was isolated as a chromosome lacking miniwhite markers in 
progeny of w[1118]; P{hs-hid}3, Dr[1]/TM6C,Sb[1] females crossed to 
P{hsFLP}1, y[1] w[1118]; P{XP}Mob2[d11543]/PBac{RB}CG32085[e02816] 
males. The males were heat shocked as larvae as described in Parks et 
al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and 
crosses in preceding and succeeding generations maintained the 
original genetic background of the Exelixis insertion stocks 
(Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). 
The recombination event generated the genetic element 
P+PBac{XP5.RB3}BSC577 from the segment of P{XP}Mob2[d11543] to the 
left of its FRT site and the segment of PBac{RB}CG32085[e02816] to 
the right of its FRT site. The cytological breakpoints of 
Df(3L)BSC577 predicted from the Release 5 genomic coordinates of the 
transposable element insertions sites are 68C13;68D1. Df(3L)BSC577 
failed to complement rt[2] and rt[P].
__________________________________________________________
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology           http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX