FlyBase Allele Report: Dmel\mwh[1]

General Information

Symbol

Dmel\mwh¹

Species

D. melanogaster

Name

FlyBase ID

FBal0012675

Feature type

allele

Associated gene

Dmel\mwh

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

amorphic allele - genetic evidence

loss of function allele

Mutagen

spontaneous

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Strutt and Warrington, 2008)

loss of function allele

(Yan et al., 2008)

Mutagen

spontaneous

(University of Milan, 1951)

Progenitor genotype

Caused by aberration

In(3L)mwh¹

Cytology

Description

Rearrangement in which an inversion breaks the coding sequence after amino acid residue 367 (this breaks within the conserved FH3 domain).

(Strutt and Warrington, 2008)

The 3' end of the mwh gene could not be amplified from mwh¹ mutants, suggesting that the mwh¹ mutation may be associated with a deletion.

(Yan et al., 2008)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

abdominal sternite bristle

(Wang et al., 2017)

actin filament

(Strutt and Warrington, 2008)

microchaeta

(Dickinson and Thatcher, 1997)

microchaeta & wing

(University of Milan, 1951)

ventral denticle belt

(Dickinson and Thatcher, 1997)

wing | pupal stage

(Pataki et al., 2010)

wing cell

(Wong and Adler, 1993)

wing hair

(Lu et al., 2010, Pataki et al., 2010, Wong and Adler, 1993)

wing hair | cell autonomous | somatic clone

(Lee and Adler, 2002)

wing hair | increased number

(Fagan et al., 2014, Gault et al., 2012, Szabad et al., 2012, Lu et al., 2010, Yan et al., 2009, Strutt and Warrington, 2008)

wing hair | increased number (with mwh⁶)

(Fagan et al., 2014)

wing hair | increased number | heat sensitive (with mwh⁶)

(Yan et al., 2009)

Detailed Description

Statement

Reference

In mwh¹ testes the number of normal individualization complexes (ICs), abnormal ICs and waste bags is similar to controls.

(Steinhauer et al., 2019)

mwh¹ homozygous adults show hair polarity defects in abdominal bristles.

(Wang et al., 2017)

mwh¹ or mwh¹/mwh⁶ flies show a 'multiple hair cell' phenotype in the wing.

(Fagan et al., 2014)

Homozygous mwh¹ mutant display wing hair defects.

(Gault et al., 2012)

Homozygotes have a multiple wing hair phenotype.

(Szabad et al., 2012)

Hairs in the proximal-anterior part of the wing point towards the anterior margin and most cells form multiple hairs in mutant adults.

(Lu et al., 2010)

Some of the mwh¹ mutant pupal wing cells fail to adopt a hexagonal shape.

mwh¹ mutants display wing hair abnormalities.

(Pataki et al., 2010)

mwh¹ animals have a multiple wing hair phenotype; the mean number of hairs/cell at 25[o]C is 3.62.

mwh¹/mwh⁶ animals have a multiple wing hair phenotype that is temperature sensitive; the mean number of hairs/cell is 1.83 at 21[o]C and 2.52 at 25[o]C.

(Yan et al., 2009)

Homozygotes show a multiple wing hair phenotype.

Prior to and during the appearance of prehair structures in the pupal wing, mutant cells show excess actin bundling across the apical surface of the cell, sometimes in a "starburst" pattern, with actin bundles radiating from the cell centre. Subsequently, prehair structures are seen at cell edges. The excess actin bundles often extend at least 1μm basally into the cytoplasm from the apical surface, particularly once prehair initiation is underway.

(Strutt and Warrington, 2008)

Mutants show no significant disruption of ovarian morphology.

(Cohen et al., 2002)

Mutant clones in the wing show disruption of polarity, as indicated by wing hairs.

(Lee and Adler, 2002)

Hairs and denticles are smaller, more numerous and less orderly than those in wild type.

(Dickinson and Thatcher, 1997)

Wing cells of homozygous flies form more than one hair, with an average of almost four hairs per cell. Homozygous flies have disruptions in the pattern of wing hair polarity. The prehair initiation site is along the cell periphery, but not restricted to the distal vertex in pupal wing cells. Double mutants of mwh¹ with fz¹, dsh¹, pk¹, in¹ or fy² resemble mwh¹ single mutants.

(Wong and Adler, 1993)

RK1 Hairs on the wings are grouped in numbers of 2-5 in the place of 1.

(University of Milan, 1951)

Affects the trichomes (hairs) of all body regions in the same general way: An increase in the number of elements is correlated with a reduction in length and a disturbance of orientation. Aristae and bracts are included in this pattern; bristles and other sensilla are not. Wing cells contain groups of 2-7 hairs instead of one hair per cell as in wild type; causes supernumerary trichomes over entire integument, but tufts of trichomes only in wing blade (Ouweneel, 1970); may be supernumerary hairs and sensilla on halteres (Ouweneel and van der Meer, 1973). Also causes disruption of polarity in legs, wings and halteres; may disrupt orientation of hairs on leg without affecting their numbers (Bryant and Schneiderman, 1969); trichomes on wings tend to diverge from vein L3 rather than parallel it as in wild type (Gubb and Garcia-Bellido, 1982). mwh/+ develop mwh phenotype following heat shock at or just prior to the time of cell-hair-extrusion (Mitchell and Petersen, 1984). Transplants of mutant wing discs to wild-type hosts develop autonomously (Ursprung and Hadorn, 1962). Widely used as a cell marker in the analysis of cuticular clones. The frequency of mwh spots after somatic recombination in mwh/+ flies increases with increase in the temperature at which the larvae and pupae are raised (Graf, 1986). RK1.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

mwh⁶/mwh¹ has visible phenotype, enhanceable by Rho1^unspecified/Rho1[+]

(Yan et al., 2009)

mwh⁶/mwh¹ has visible phenotype, enhanceable by Scer\GAL4^ptc-559.1/RhoGAPp190^UAS.Tag:MYC

(Yan et al., 2009)

NOT Enhanced by

Statement

Reference

mwh¹ has visible | recessive phenotype, non-enhanceable by Rab23⁵¹

(Pataki et al., 2010)

mwh¹ has abnormal planar polarity | somatic clone phenotype, non-enhanceable by grh^IM

(Lee and Adler, 2004)

Suppressed by

Statement

Reference

in^IH56, mwh¹ has abnormal planar polarity phenotype, suppressible | partially by in::mwh^UAS.cLa/Scer\GAL4^ap-md544

(Lu et al., 2010)

mwh¹ has abnormal planar polarity phenotype, suppressible by Scer\GAL4^Act.PU/in::mwh^UAS.cLa

(Lu et al., 2010)

mwh⁶/mwh¹ has visible phenotype, suppressible | partially by Scer\GAL4^ap-md544/Rho1^UAS.GFP

(Yan et al., 2009)

mwh⁶/mwh¹ has visible phenotype, suppressible | partially by Scer\GAL4^ptc-559.1/Rho1^V14.UAS

(Yan et al., 2009)

mwh⁶/mwh¹ has visible phenotype, suppressible | partially by Scer\GAL4^ptc-559.1/RhoGEF2^UAS.cUa

(Yan et al., 2009)

mwh⁶/mwh¹ has visible phenotype, suppressible | partially by Scer\GAL4^ptc-559.1/Rok^CAT.UAS

(Yan et al., 2009)

mwh¹ has visible phenotype, suppressible by Scer\GAL4^ap-md544/Rho1^UAS.GFP

(Yan et al., 2009)

NOT suppressed by

Statement

Reference

mwh¹ has visible | recessive phenotype, non-suppressible by Rab23⁵¹

(Pataki et al., 2010)

mwh⁶/mwh¹ has visible phenotype, non-suppressible by Scer\GAL4^ptc-559.1/Rok^CAT-KG.UAS

(Yan et al., 2009)

mwh¹ has abnormal planar polarity | somatic clone phenotype, non-suppressible by grh^IM

(Lee and Adler, 2004)

Enhancer of

Statement

Reference

mwh[+]/mwh¹ is an enhancer of visible phenotype of Scer\GAL4^en-e16E, kermit^GS2053

(Djiane and Mlodzik, 2010)

mwh[+]/mwh¹ is an enhancer of visible | recessive phenotype of Rab23⁵¹

(Pataki et al., 2010)

NOT Enhancer of

Statement

Reference

mwh¹ is a non-enhancer of abnormal planar polarity | somatic clone phenotype of grh^IM

(Lee and Adler, 2004)

Suppressor of

Statement

Reference

mwh¹ is a suppressor of abnormal planar polarity | somatic clone | cell non-autonomous phenotype of fz²³

(Lee and Adler, 2002)

NOT Suppressor of

Statement

Reference

mwh¹ is a non-suppressor of male sterile | recessive phenotype of cmb^KO

(Steinhauer et al., 2019)

mwh¹ is a non-suppressor of abnormal planar polarity | somatic clone phenotype of grh^IM

(Lee and Adler, 2004)

mwh¹ is a non-suppressor of abnormal planar polarity | somatic clone | cell autonomous phenotype of fz²³

(Lee and Adler, 2002)

mwh¹ is a non-suppressor of abnormal planar polarity | somatic clone | cell non-autonomous phenotype of fz²³

(Lee and Adler, 2002)

Phenotype Manifest In

Enhanced by

Statement

Reference

mwh¹ has wing hair | increased number phenotype, enhanceable by Scer\GAL4^en-e16E/gish^GD10588

(Gault et al., 2012)

mwh⁶/mwh¹ has wing hair | increased number phenotype, enhanceable by Rho1^unspecified/Rho1[+]

(Yan et al., 2009)

mwh⁶/mwh¹ has wing hair | increased number phenotype, enhanceable by Scer\GAL4^ptc-559.1/RhoGAPp190^UAS.Tag:MYC

(Yan et al., 2009)

mwh¹ has wing hair | somatic clone phenotype, enhanceable by ultA¹

(Adler et al., 2000)

NOT Enhanced by

Statement

Reference

mwh¹ has wing hair phenotype, non-enhanceable by Rab23⁵¹

(Pataki et al., 2010)

mwh¹ has wing hair | somatic clone phenotype, non-enhanceable by grh^IM

(Lee and Adler, 2004)

mwh¹ has wing hair | increased number | somatic clone phenotype, non-enhanceable by grh^IM

(Lee and Adler, 2004)

Suppressed by

Statement

Reference

mwh⁶/mwh¹ has wing hair | increased number phenotype, suppressible by cmb^KO

(Fagan et al., 2014)

mwh¹ has wing hair | increased number phenotype, suppressible by cmb^KO

(Fagan et al., 2014)

in^IH56, mwh¹ has wing hair phenotype, suppressible | partially by in::mwh^UAS.cLa/Scer\GAL4^ap-md544

(Lu et al., 2010)

in^IH56, mwh¹ has wing hair | increased number phenotype, suppressible | partially by in::mwh^UAS.cLa/Scer\GAL4^ap-md544

(Lu et al., 2010)

mwh¹ has wing hair phenotype, suppressible by Scer\GAL4^Act.PU/in::mwh^UAS.cLa

(Lu et al., 2010)

mwh¹ has wing hair | increased number phenotype, suppressible by Scer\GAL4^Act.PU/in::mwh^UAS.cLa

(Lu et al., 2010)

mwh⁶/mwh¹ has wing hair | increased number phenotype, suppressible | partially by Scer\GAL4^ap-md544/Rho1^UAS.GFP

(Yan et al., 2009)

mwh⁶/mwh¹ has wing hair | increased number phenotype, suppressible | partially by Scer\GAL4^ptc-559.1/Rho1^V14.UAS

(Yan et al., 2009)

mwh⁶/mwh¹ has wing hair | increased number phenotype, suppressible | partially by Scer\GAL4^ptc-559.1/RhoGEF2^UAS.cUa

(Yan et al., 2009)

mwh⁶/mwh¹ has wing hair | increased number phenotype, suppressible | partially by Scer\GAL4^ptc-559.1/Rok^CAT.UAS

(Yan et al., 2009)

mwh¹ has wing hair | increased number phenotype, suppressible by Scer\GAL4^ap-md544/Rho1^UAS.GFP

(Yan et al., 2009)

NOT suppressed by

Statement

Reference

mwh¹ has wing hair phenotype, non-suppressible by Rab23⁵¹

(Pataki et al., 2010)

mwh⁶/mwh¹ has wing hair | increased number phenotype, non-suppressible by Scer\GAL4^ptc-559.1/Rok^CAT-KG.UAS

(Yan et al., 2009)

mwh¹ has wing hair | somatic clone phenotype, non-suppressible by grh^IM

(Lee and Adler, 2004)

mwh¹ has wing hair | increased number | somatic clone phenotype, non-suppressible by grh^IM

(Lee and Adler, 2004)

Enhancer of

Statement

Reference

mwh[+]/mwh¹ is an enhancer of wing hair | increased number phenotype of Scer\GAL4^en.PU, cmb^{UAS.RA.Tag:FLAG}

(Fagan et al., 2014)

mwh[+]/mwh¹ is an enhancer of wing hair | increased number | somatic clone phenotype of gish^e01759

(Gault et al., 2012)

mwh[+]/mwh¹ is an enhancer of wing hair phenotype of Scer\GAL4^en-e16E, kermit^GS2053

(Djiane and Mlodzik, 2010)

mwh[+]/mwh¹ is an enhancer of wing hair phenotype of Rab23⁵¹

(Pataki et al., 2010)

mwh[+]/mwh¹ is an enhancer of wing hair & 1st posterior cell phenotype of in^II53

(Lee and Adler, 2002)

mwh[+]/mwh¹ is an enhancer of wing hair & 1st posterior cell phenotype of fy²

(Lee and Adler, 2002)

mwh[+]/mwh¹ is an enhancer of wing hair & 1st posterior cell phenotype of in¹

(Lee and Adler, 2002)

mwh¹ is an enhancer of wing hair | somatic clone phenotype of ultA¹

(Adler et al., 2000)

NOT Enhancer of

Statement

Reference

mwh¹ is a non-enhancer of wing hair | somatic clone phenotype of grh^IM

(Lee and Adler, 2004)

mwh¹ is a non-enhancer of wing hair | increased number | somatic clone phenotype of grh^IM

(Lee and Adler, 2004)

Suppressor of

Statement

Reference

mwh¹ is a suppressor of wing hair | somatic clone | cell non-autonomous phenotype of fz²³

(Lee and Adler, 2002)

NOT Suppressor of

Statement

Reference

mwh¹ is a non-suppressor of wing hair | somatic clone phenotype of grh^IM

(Lee and Adler, 2004)

mwh¹ is a non-suppressor of wing hair | increased number | somatic clone phenotype of grh^IM

(Lee and Adler, 2004)

mwh¹ is a non-suppressor of wing hair | somatic clone | cell autonomous phenotype of fz²³

(Lee and Adler, 2002)

mwh¹ is a non-suppressor of wing hair | somatic clone | cell non-autonomous phenotype of fz²³

(Lee and Adler, 2002)

Additional Comments

Genetic Interactions

Statement

Reference

Heterozygosity significantly enhances the multiple hair cell phenotype of Scer\GAL4^en.PU cmb^Scer\UAS.RA wings.

Homozygosity for cmb^KO significantly suppresses the multiple hair cell phenotype of mwh¹ and mwh¹/mwh⁶ wings.

(Fagan et al., 2014)

mwh¹ dominantly enhances the trichome-phenotype of homozygous gish^e01759 mutant clones in the wing.

Expression of gish^GD10588 via Scer\GAL4^en-e16E strongly enhances the wing hair phenotype associated with homozygous mwh¹.

(Gault et al., 2012)

The wing hair phenotype of in^IH56 mwh¹ double mutants is indistinguishable from that of mwh¹ single mutants.

Expression of in::mwh^Scer\UAS.cLa under the control of Scer\GAL4^Act.PU almost completely rescues the wing hair defects of mwh¹ mutants.

Expression of in::mwh^Scer\UAS.cLa under the control of Scer\GAL4^ap-md544 strongly rescues the wing hair defects of in^IH56 mwh¹ double mutants on the dorsal surface of the wing.

(Lu et al., 2010)

The mwh¹ single mutant wing hair phenotype is essentially identical to the mwh¹ , Rab23⁵¹ double mutant phenotype.

mwh¹ dominantly enhances the multiple wing hair phenotype of Rab23⁵¹ mutants.

(Pataki et al., 2010)

Expression of Rho1^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^ap-md544 partially but significantly suppresses the mwh¹ multiple wing hair phenotype.

Expression of Rho1^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^ap-md544 weakly suppresses the mwh¹/mwh⁶ multiple wing hair phenotype.

Low levels of expression of Rho1^V14.Scer\UAS under the control of Scer\GAL4^ptc-559.1 at 25[o]C (the flies also carry Scer\GAL80^ts.αTub84B) partially suppress the mwh¹/mwh⁶ multiple wing hair phenotype.

Expression of RhoGEF2^Scer\UAS.cUa under the control of Scer\GAL4^ptc-559.1 partially suppresses the mwh¹/mwh⁶ multiple wing hair phenotype.

The mwh¹/mwh⁶ multiple wing hair phenotype is dominantly enhanced by Rho1^unspecified.

Expression of RhoGAPp190^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^ptc-559.1 enhances the mwh¹/mwh⁶ multiple wing hair phenotype.

Expression of rok^CAT.Scer\UAS under the control of Scer\GAL4^ptc-559.1 partially suppresses the mwh¹/mwh⁶ multiple wing hair phenotype.

Expression of rok^{CAT-KG.Scer\UAS} under the control of Scer\GAL4^ptc-559.1 does not suppress the mwh¹/mwh⁶ multiple wing hair phenotype.

(Yan et al., 2009)

When grh^IM mutant clones are made in an mwh¹ mutant wing the grh^IM phenotype is still seen. A slight additive effect is seen on the phenotype.

(Lee and Adler, 2004)

In somatic clones of cells that are homozygous for both mwh¹ and ultA¹, an increase is seen in the average number of wing hairs per cell.

(Adler et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

mwh¹ is rescued by mwh^+t38

(Szabad et al., 2012)

mwh¹ is rescued by mwh^Act5C.EGFP

(Strutt and Warrington, 2008)

Comments

Images (0)

Mutant

Wild-type

Stocks (243)

Bloomington

549

mwh¹

5747

Dp(3;3)Cam37, mwh¹/MKRS

2558

mwh¹ ry⁵⁰⁶ e¹

3678

Df(3R)sbd45, mwh¹ e¹/TM6

479

43636

101704

105974

101892

108403

Dp(3;3)Cam37, mwh¹/TM1

107402

mwh¹ ry⁵⁰⁶ e¹

107170

Df(3R)sbd45, mwh¹ e^1?/TM6

More stocks available...

Notes on Origin

Discoverer

Comments

Isolated from Moltrasio.

(University of Milan, 1951)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

CG43772¹

mwh

(University of Milan, 1951)

mwh¹

(Brown et al., 2020, Steinhauer et al., 2019, Chavez et al., 2017, Wang et al., 2017, Lu et al., 2015, Fagan et al., 2014, Warrington et al., 2013, Gault et al., 2012, Szabad et al., 2012, Pardi et al., 2011, Djiane and Mlodzik, 2010, Lu et al., 2010, Papaconstantinou et al., 2010, Pataki et al., 2010, Yan et al., 2009, Strutt and Warrington, 2008, Yan et al., 2008, Jafar-Nejad et al., 2006)

Name Synonyms

multiple wing hairs

(University of Milan, 1951)

Secondary FlyBase IDs

References (32)

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