FlyBase Allele Report: Dmel\RhoGAPp190[UAS.Tag:MYC]

General Information

Symbol

Dmel\RhoGAPp190^UAS.Tag:MYC

Species

D. melanogaster

Name

FlyBase ID

FBal0128101

Feature type

allele

Associated gene

Dmel\RhoGAPp190

Associated Insertion(s)

Carried in Construct

P{UAS-myc-RhoGAPp190.B}

Also Known As

UAS-mycp190

Key Links

Transgenic product class

wild_type

(Billuart et al., 2001)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Billuart et al., 2001)

Mutagen

in vitro construct

(Billuart et al., 2001)

Progenitor genotype

Carried in construct

P{UAS-myc-RhoGAPp190.B}

Cytology

Description

UASt regulatory sequences drive expression of RhoGAPp190 tagged with Tag:MYC.

(Billuart et al., 2001)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

RhoGAPp190

Tagged with

Tag:MYC

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^ey-OK107

(Ng and Luo, 2004, Billuart et al., 2001)

Phenotype Manifest In

adult mushroom body, with Scer\GAL4^ey-OK107

(Ng and Luo, 2004)

vertical lobe of adult mushroom body, with Scer\GAL4^ey-OK107

(Billuart et al., 2001)

wing hair | increased number, with Scer\GAL4^ptc-559.1

(Yan et al., 2009)

Detailed Description

Statement

Reference

Expression of RhoGAPp190^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^ptc-559.1 results in occasional cells with multiple hairs in the wing.

(Yan et al., 2009)

Expression of RhoGAPp190^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^OK107 results in axon growth defects in the mushroom body.

(Ng and Luo, 2004)

Expression of RhoGAPp190^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^OK107 results in overextension of the dorsal lobe axons in the mushroom body.

(Billuart et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Scer\GAL4^ptc-559.1/RhoGAPp190^UAS.Tag:MYC is an enhancer of visible phenotype of mwh⁶/mwh¹

(Yan et al., 2009)

Suppressor of

Statement

Reference

RhoGAPp190^UAS.Tag:MYC, Scer\GAL4^en-e16E is a suppressor of visible phenotype of LIMK1^UAS.cCa, Scer\GAL4^en-e16E

(Chen et al., 2004)

RhoGAPp190^UAS.Tag:MYC, Scer\GAL4^ey-OK107 is a suppressor of abnormal neuroanatomy phenotype of LIMK1^UAS.Tag:HA, Scer\GAL4^ey-OK107

(Ng and Luo, 2004)

Phenotype Manifest In

Enhancer of

Statement

Reference

Scer\GAL4^ptc-559.1/RhoGAPp190^UAS.Tag:MYC is an enhancer of wing hair | increased number phenotype of mwh⁶/mwh¹

(Yan et al., 2009)

Suppressor of

Statement

Reference

RhoGAPp190^UAS.Tag:MYC, Scer\GAL4^en-e16E is a suppressor of wing phenotype of LIMK1^UAS.cCa, Scer\GAL4^en-e16E

(Chen et al., 2004)

RhoGAPp190^UAS.Tag:MYC, Scer\GAL4^ey-OK107 is a suppressor of adult mushroom body phenotype of LIMK1^UAS.Tag:HA, Scer\GAL4^ey-OK107

(Ng and Luo, 2004)

Other

Statement

Reference

LIMK1^UAS.cCa, RhoGAPp190^UAS.Tag:MYC, Scer\GAL4^en-e16E has crossvein | ectopic phenotype

(Chen et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of RhoGAPp190^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^ptc-559.1 enhances the mwh¹/mwh⁶ multiple wing hair phenotype.

(Yan et al., 2009)

Co-expression of RhoGAPp190^{Scer\UAS.T:Hsap\MYC} suppresses the mutant wing phenotype caused by expression of LIMK1^Scer\UAS.cCa under the control of Scer\GAL4^en-e16E (the % of wings with normal morphology at 18^oC is increased from 9% to 85%). Approximately 63% of the flies show a mild extra crossvein phenotype.

(Chen et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

RhoGAPp190^UAS.Tag:MYC is rescued by Scer\GAL4^elav.PLu/RhoGAPp190^UAS.Tag:MYC

(Jeong et al., 2012)

Rescues

Scer\GAL4^elav.PLu/RhoGAPp190^UAS.Tag:MYC rescues RhoGAPp190^UAS.Tag:MYC

(Jeong et al., 2012)

Partially rescues

Scer\GAL4^elav.PLu/RhoGAPp190^UAS.Tag:MYC partially rescues RhoGAPp190²

(Jeong et al., 2012)

Comments

Co-expression of RhoGAPp190^{Scer\UAS.T:Hsap\MYC} rescues the premature defasciculation phenotype seen in the ISNb axons in embryos expressing RhoGAPp190^{dsRNA.GAP.Scer\UAS} under the control of Scer\GAL4^elav.PLu.

Expression of RhoGAPp190^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^elav.PLu partially rescues the ISNb axon guidance defects seen in embryos both maternally and zygotically mutant for RhoGAPp190².

(Jeong et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

6684

y¹ w^*; P{UAS-myc-RhoGAPp190.B}2.1

25769

y¹ w^*; P{UAS-myc-RhoGAPp190.B}8.1/TM3, Sb¹

Notes on Origin

Discoverer