FlyBase Allele Report: Dmel\Doa[01705b]

General Information

Symbol

Dmel\Doa^01705b

Species

D. melanogaster

Name

FlyBase ID

FBal0009424

Feature type

allele

Associated gene

Dmel\Doa

Associated Insertion(s)

P{PZ}Doa^01705b

Carried in Construct

Also Known As

l(3)01705

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

Progenitor genotype

Associated Insertion(s)

P{PZ}Doa^01705b

Cytology

Description

The P{PZ} element is inserted 131 nucleotides 5' to the first non-coding exon of the 105kDa Doa coding transcript and 1667 nucleotides 5' to its initiating AUG.

(Kpebe and Rabinow, 2008)

Allele components

Component

Use(s)

Inserted element

P{PZ}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:28,891,765..28,891,765

(Kpebe and Rabinow, 2008, Yun et al., 2000, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal (with Doa^3B)

(Yun et al., 2000)

lethal (with Doa^5d7)

(Ambrus et al., 2009)

lethal (with Doa^5d19)

(Ambrus et al., 2009)

lethal (with Doa^6a21)

(Ambrus et al., 2009)

lethal (with Doa^6a52)

(Ambrus et al., 2009)

lethal (with Doa^6d2)

(Ambrus et al., 2009)

lethal (with Doa^HD)

(Yun et al., 2000)

lethal | recessive

(Spradling et al., 1999)

visible (with Doa⁷)

(Yun et al., 2000)

visible (with Doa^Dem)

(Yun et al., 2000)

visible (with In(3)Msu¹)

(Yun et al., 2000)

visible (with In(3)Msu²)

(Yun et al., 2000)

visible | adult stage (with Doa^Dem)

(Kpebe and Rabinow, 2008)

Phenotype Manifest In

mesothoracic leg (with Doa^Dem)

(Kpebe and Rabinow, 2008)

metathoracic leg (with Doa^Dem)

(Kpebe and Rabinow, 2008)

ommatidium (with Doa⁷)

(Yun et al., 2000)

ommatidium (with Doa^Dem)

(Yun et al., 2000)

ommatidium (with In(3)Msu¹)

(Yun et al., 2000)

ommatidium (with In(3)Msu²)

(Yun et al., 2000)

scutellar bristle | increased number (with Doa⁷)

(Yun et al., 2000)

scutellar bristle | increased number (with Doa^Dem)

(Yun et al., 2000)

scutellar bristle | increased number (with In(3)Msu¹)

(Yun et al., 2000)

scutellar bristle | increased number (with In(3)Msu²)

(Yun et al., 2000)

Detailed Description

Statement

Reference

In Doa^Dem/Doa^01705b mutant flies, the leg segments distal to the first tarsus often fail to elongate. The femur and claws form normally. Defects are seen in both the mesothoracic and metathoracic tarsi.

In Doa^Su(Mrt9)/Doa^01705b mutants where the Doa^Su(Mrt9) allele is from the mother more females are observed than males. The flies have rough eyes, loss of head, scutellar or postalar bristles, fragile wings with small black spots and/or ectopic wing veins and disorganisation of the vaginal bristles. The flies have very rough eyes, some misoriented head bristles and disorganisation of the vaginal bristles. When the Doa^01705b allele comes from the mother there are no survivors.

(Kpebe and Rabinow, 2008)

Transheterozygotes with Doa⁷, In(3)Msu¹, In(3)Msu² or Doa^Dem have rough eyes and duplicated scutellar bristles.

(Yun et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressor of

Statement

Reference

Doa^01705b is a suppressor of phenotype of w^a

(Rabinow, 1995.5.24)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Doa⁰⁴⁷⁴³

(BDGP Project Members, 1994-1999)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

A. Spradling.

Separable from: P{PZ}01705a. Evidence suggests that the "l(3)01705" line contains 2 P{PZ} insertions; one at 39B (P{PZ}01705a) corresponding to the site of the STS sequence from this line (G00743), and one at 98F (P{PZ}Doa^01705b) which causes a mutation in Doa.

Comments

Complements: stg¹⁴.

(BDGP Project Members, 1994-1999)

Fails to complement the recessive lethality of some, but not all, alleles of Doa.

(Rabinow, 1995.5.24)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Doa⁰¹⁷⁰⁵

Doa^01705b

(Ambrus et al., 2009)

l(3)01705

(Yun et al., 2000, Spradling et al., 1999)

l(3)01705⁰¹⁷⁰⁵

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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