FlyBase Allele Report: Dmel\pum[01688]

General Information

Symbol

Dmel\pum⁰¹⁶⁸⁸

Species

D. melanogaster

Name

FlyBase ID

FBal0009423

Feature type

allele

Associated gene

Dmel\pum

Associated Insertion(s)

P{PZ}pum⁰¹⁶⁸⁸

Carried in Construct

Also Known As

pum¹⁶⁸⁸

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Lin and Spradling, 1997)

Progenitor genotype

Associated Insertion(s)

P{PZ}pum⁰¹⁶⁸⁸

Cytology

Description

P{PZ} insertion in the intron between exons 3 and 4. The orientation of the P{PZ} element is such that the ry marker gene is towards the 5' side of the pum gene while the Ecol\lacZ gene is oriented towards the 3' side.

(Parisi and Lin, 1999)

Allele components

Component

Use(s)

Inserted element

P{PZ}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:9,226,838..9,226,838 [+]

(Schweers et al., 2002, Parisi and Lin, 1999, Spradling, 1999.12.9, Spradling et al., 1999, Lin and Spradling, 1997, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates oculopharyngeal muscular dystrophy

modeled by Hsap\PABPN1^17ala.Act88F

(Chartier et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell number | adult stage | female

(Parisi and Lin, 1999)

decreased fecundity | female

(Parisi and Lin, 1999)

female sterile

(Parisi and Lin, 1999, Lin and Spradling, 1997)

increased occurrence of cell division | third instar larval stage

(Parisi and Lin, 1999)

lethal | recessive

(Spradling et al., 1999)

partially lethal - majority die

visible (with pum¹)

(Kim et al., 2012)

visible (with pum³)

(Kim et al., 2012)

visible (with pum⁷)

(Kim et al., 2012)

visible (with pum^Msc)

(Kim et al., 2012)

visible | recessive

(Kim et al., 2012)

Phenotype Manifest In

egg chamber

(Parisi and Lin, 1999)

female germline stem cell | adult stage

(Parisi and Lin, 1999)

female germline stem cell | larval stage

(Parisi and Lin, 1999)

germarium

(Parisi and Lin, 1999)

macrochaeta | ectopic

(Kim et al., 2012)

macrochaeta | ectopic (with pum³)

(Kim et al., 2012)

macrochaeta | ectopic (with pum⁷)

(Kim et al., 2012)

metathoracic tergum

(Kim et al., 2012)

metathoracic tergum (with pum³)

(Kim et al., 2012)

metathoracic tergum (with pum⁷)

(Kim et al., 2012)

motor neuron

(Kraut et al., 2001)

nurse cell

(Parisi and Lin, 1999)

oocyte

(Parisi and Lin, 1999)

ovariole | germline clone

(Parisi and Lin, 1999)

ovary

(Lin and Spradling, 1997)

ovary | adult stage

(Parisi and Lin, 1999)

ovary | third instar larval stage

(Parisi and Lin, 1999)

wing vein | ectopic

(Kim et al., 2012)

wing vein | ectopic (with pum¹)

(Kim et al., 2012)

wing vein | ectopic (with pum³)

(Kim et al., 2012)

wing vein | ectopic (with pum⁷)

(Kim et al., 2012)

wing vein | ectopic (with pum^Msc)

(Kim et al., 2012)

Detailed Description

Statement

Reference

Homozygous pum⁰¹⁶⁸⁸ escapers have rare extra wing veins. Trans-heterozygous pum⁷/pum⁰¹⁶⁸⁸ flies also display extra wing veins.

Trans-heterozygotes of pum¹/pum⁰¹⁶⁸⁸ display extra wing veins.

Trans-heterozygotes of pum³/pum⁰¹⁶⁸⁸ display extra wing veins and extra macrochaetae on their notum. The penetrance of the notum phenotype is incomplete.

Trans-heterozygotes of pum^Msc/pum⁰¹⁶⁸⁸ display extra wing veins and extra macrochaetae on their notum. The penetrance of the notum phenotype is incomplete.

The notum of pum⁷/pum⁰¹⁶⁸⁸ escapers display extra macrochaetae. The penetrance of the notum phenotype is incomplete.

The notum of homozygous pum⁰¹⁶⁸⁸ escapers display extra macrochaetae. The penetrance of the notum phenotype is incomplete.

(Kim et al., 2012)

Mutants have a motor axon pathfinding phenotype.

(Kraut et al., 2001)

Homozygous females do not lay eggs. Most homozygous larvae survive the pupal stage to eclosion. 40% of homozygous ovaries from third instar larvae have a normal number of germline cells and 60% have an overproliferated germline. By the adult stage, 9.5% of homozygous females have germlineless ovaries, 62% have "differentiated ovaries" (containing recognisable egg chambers and/or germline cells in at least one ovariole) and 28.5% of females have one germlineless and one differentiated ovary. Developing mutant egg chambers sometimes contain few nurse cells but no oocytes. Homozygous female germline clones induced at the third larval instar stage produce "differentiated" ovarioles that contain on average 2.8 +/- 1.7 mature egg chambers. Ovarioles containing a single mature egg and a rudimentary germarium are also seen. Females containing homozygous female germline clones achieve an oviposition efficiency of 85% (for absolute number of eggs laid by the females). These eggs are (derived from females containing homozygous germline clones mated to homozygous males) sometimes capable of developing into adulthood.

(Parisi and Lin, 1999)

Small ovary phenotype.

(Lin and Spradling, 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

pum⁰¹⁶⁸⁸ is an enhancer of visible phenotype of Scer\GAL4^bbg-C96, dom^RNAi.Sym.UAS

(Kwon et al., 2013)

pum⁰¹⁶⁸⁸/pum[+] is an enhancer of visible phenotype of Egfr^UAS.cUa, Scer\GAL4^sca-537.4

(Kim et al., 2012)

NOT Enhancer of

Statement

Reference

pum⁰¹⁶⁸⁸ is a non-enhancer of abnormal neuroanatomy phenotype of Hsap\ATXN8OS^CTG112.UAS, Scer\GAL4^GMR.PF

(Mutsuddi et al., 2004)

Suppressor of

Statement

Reference

pum⁰¹⁶⁸⁸/pum[+] is a suppressor of visible phenotype of Hsap\PABPN1^17ala.Act88F

(Chartier et al., 2015)

NOT Suppressor of

Statement

Reference

pum⁰¹⁶⁸⁸ is a non-suppressor of abnormal neuroanatomy phenotype of Hsap\ATXN8OS^CTG112.UAS, Scer\GAL4^GMR.PF

(Mutsuddi et al., 2004)

Phenotype Manifest In

Enhancer of

Statement

Reference

pum⁰¹⁶⁸⁸ is an enhancer of wing margin phenotype of Scer\GAL4^bbg-C96, dom^RNAi.Sym.UAS

(Kwon et al., 2013)

pum⁰¹⁶⁸⁸/pum[+] is an enhancer of metathoracic tergum phenotype of Egfr^UAS.cUa, Scer\GAL4^sca-537.4

(Kim et al., 2012)

pum⁰¹⁶⁸⁸/pum[+] is an enhancer of macrochaeta | ectopic phenotype of Egfr^UAS.cUa, Scer\GAL4^sca-537.4

(Kim et al., 2012)

Suppressor of

Statement

Reference

pum⁰¹⁶⁸⁸/pum[+] is a suppressor of wing phenotype of Hsap\PABPN1^17ala.Act88F

(Chartier et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The wing nicking phenotype caused by expression of dom^{dsRNA.Sym.Scer\UAS} under the control of Scer\GAL4^bbg-C96 is enhanced if the flies are also carrying pum⁰¹⁶⁸⁸.

(Kwon et al., 2013)

Heterozygous pum⁰¹⁶⁸⁸ greatly enhances the ectopic macrochaeta phenotype induced by Egfr^Scer\UAS.cUa overexpression in the notum under the control of Scer\GAL4^sca-537.4.

(Kim et al., 2012)

Xenogenetic Interactions

Statement

Reference

pum⁰¹⁶⁸⁸ suppresses the wing posture defects seen upon expression of Hsap\PABPN1^17ala.Act88F.

(Chartier et al., 2015)

Complementation and Rescue Data

Complements

pum^bem

(Schweers et al., 2002)

pum^rK404

(BDGP Project Members, 1994-1999)

Partially complements

pum⁷

(Parisi and Lin, 1999)

pum¹

(Parisi and Lin, 1999)

pum^Msc

(Parisi and Lin, 1999)

pum⁰³²⁰³

(Parisi and Lin, 1999)

pum⁰²⁰⁰³

(Parisi and Lin, 1999)

Fails to complement

pum^rK404

(BDGP Project Members, 1994-1999)

pum¹⁰⁶²⁵

(BDGP Project Members, 1994-1999)

pum⁰⁴³³⁸

(BDGP Project Members, 1994-1999)

pum⁰³²⁰³

(BDGP Project Members, 1994-1999)

Rescued by

pum⁰¹⁶⁸⁸ is rescued by pum^nanos.PB

(Parisi and Lin, 1999)

Comments

Complementation tested against pum^bem female sterility phenotype and gravitaxis and flight defects.

(Schweers et al., 2002)

Partially complements the embryonic lethality of the maternal effect alleles pum¹, pum^Msc and pum⁷, resulting in viable yet sterile progeny. Partially complements pum⁰²⁰⁰³ and pum⁰³²⁰³ for oogenic defects and embryonic lethality.

(Parisi and Lin, 1999)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

11544

P{PZ}pum⁰¹⁶⁸⁸ ry⁵⁰⁶/TM3, ry^RK Sb¹ Ser¹

Notes on Origin

Discoverer

A. Spradling.

Comments

Complements: l(3)01728⁰¹⁷²⁸. Complements: Aats-trp⁰³⁵⁵⁹. Complements: Aats-trp⁰⁴⁴¹⁰. Complements: l(3)04837⁰⁴⁸³⁷. Complements: l(3)05430⁰⁵⁴³⁰. Complements: Ras85D⁰⁶⁶⁷⁷. Complements: neur^neo37.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

l(3)01688

(Spradling, 1999.12.9, Spradling et al., 1999)

l(3)01688⁰¹⁶⁸⁸

pum⁰¹⁶⁸⁸

(Saha et al., 2023, Chartier et al., 2015, Joly et al., 2013, Harris et al., 2011)

pum¹⁶⁸⁸

(Kim et al., 2012, Szakmary et al., 2005, Schweers et al., 2002, Parisi and Lin, 1999)

pumilio⁰¹⁶⁸⁸

(Kwon et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (17)

Report Sections

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