FlyBase Allele Report: Dmel\pum[03203]

General Information

Symbol

Dmel\pum⁰³²⁰³

Species

D. melanogaster

Name

FlyBase ID

FBal0043646

Feature type

allele

Associated gene

Dmel\pum

Associated Insertion(s)

P{PZ}pum⁰³²⁰³

Carried in Construct

Also Known As

pum³²⁰³

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Lin and Spradling, 1997, Schmucker et al., 1997)

Progenitor genotype

Associated Insertion(s)

P{PZ}pum⁰³²⁰³

Cytology

Description

The P{lacW} transposon in inserted between exons 8 and 9 on the pum transcription unit.

(Schweers et al., 2002)

P{PZ} insertion in the intron between exons 8 and 9. The orientation of the P{PZ} element is such that the ry marker gene is towards the 5' side of the pum gene while the Ecol\lacZ gene is oriented towards the 3' side.

(Parisi and Lin, 1999)

Allele components

Component

Use(s)

Inserted element

P{PZ}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:9,158,090..9,158,090 [+]

(Parisi and Lin, 1999, Lin and Spradling, 1997, Schmucker et al., 1997, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell number | adult stage | female

(Parisi and Lin, 1999)

decreased cell number | third instar larval stage

(Parisi and Lin, 1999)

female sterile

(Lin and Spradling, 1997)

partially lethal - majority die

(Schmucker et al., 1997)

Phenotype Manifest In

Bolwig nerve

(Schmucker et al., 1997)

female germline stem cell | adult stage

(Parisi and Lin, 1999)

female germline stem cell | larval stage

(Parisi and Lin, 1999)

ovary

(Lin and Spradling, 1997)

ovary | adult stage

(Parisi and Lin, 1999)

Detailed Description

Statement

Reference

55% of homozygous ovaries from third instar larvae have an underproliferated germline and 45% have a normal number of germline cells. By the adult stage, 31% of homozygous females have germlineless ovaries, 31% have "differentiated ovaries" (containing recognisable egg chambers and/or germline cells in at least one ovariole) and 38% of females have one germlineless and one differentiated ovary.

(Parisi and Lin, 1999)

Small ovary phenotype.

(Lin and Spradling, 1997)

The Bolwig's nerve (BN) is usually properly fasciculated and follows its normal path until it approaches P2, where it undergoes terminal sprouting, leading to the formation of several independent nerve branches which remain on the brain surface. Defasciculation of the BN is seen prior to reaching P2 in about 20% of embryos.

(Schmucker et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially complements

pum⁰¹⁶⁸⁸

(Parisi and Lin, 1999)

Fails to complement

pum^bem

(Schweers et al., 2002)

pum⁰¹⁶⁸⁸

(BDGP Project Members, 1994-1999)

pum⁰⁴³³⁸

(BDGP Project Members, 1994-1999)

Comments

Complementation tested against pum^bem female sterility phenotype. pum⁰³²⁰³ was also tested against the pum^bem gravitaxis and flight defects, and found not to complement.

(Schweers et al., 2002)

pum⁰¹⁶⁸⁸ partially complements pum⁰³²⁰³ for oogenic defects and embryonic lethality.

(Parisi and Lin, 1999)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

A. Spradling.

Comments

Complements: l(3)01728⁰¹⁷²⁸. Complements: Aats-trp⁰³⁵⁵⁹. Complements: l(3)04837⁰⁴⁸³⁷. Complements: l(3)05430⁰⁵⁴³⁰.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

l(3)03203

(Schmucker et al., 1997)

l(3)03203⁰³²⁰³

pum⁰³²⁰³

pum³²⁰³

(Schweers et al., 2002, Parisi and Lin, 1999)

pum^ovt

(Schweers et al., 2002)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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