FlyBase Gene Report: Dmel\Thg

General Information

Symbol

Dmel\Thg

Species

D. melanogaster

Name

tRNA-histidine guanylyltransferase

Annotation Symbol

CG4103

Feature Type

protein_coding_gene

FlyBase ID

FBgn0283659

Gene Model Status

Current

Stock Availability

11 publicly available

Enzyme Name (EC)

tRNA(His) guanylyltransferase (2.7.7.79)

Gene Summary

Adds a GMP to the 5'-end of tRNA(His) after transcription and RNase P cleavage. (UniProt, Q9V3N8)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

l(2)br4, BG:DS00929.4 , br4, l(2)35Bc, l(2)br11

Key Links

Genomic Location

Cytogenetic map

35B8-35B8

Sequence location

2L:15,008,489..15,009,724 [-]

Recombination map

2-50

RefSeq locus

NT_033779 REGION:15008489..15009724

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (4 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables magnesium ion binding

inferred from electronic annotation with InterPro:IPR007537, InterPro:IPR024956

(InterPro Project Members, 2004-)

enables tRNA guanylyltransferase activity

inferred from sequence or structural similarity with UniProtKB:P53215

(Gene Ontology Curators, 2002-)

inferred from sequence or structural similarity with UniProtKB:Q9NWX6

(Gene Ontology Curators, 2002-)

inferred from biological aspect of ancestor with PANTHER:PTN000977687

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR007537, InterPro:IPR024956

(InterPro Project Members, 2004-)

Biological Process (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in tRNA modification

inferred from electronic annotation with InterPro:IPR007537, InterPro:IPR024956

(InterPro Project Members, 2004-)

inferred from sequence or structural similarity with UniProtKB:P53215

(Gene Ontology Curators, 2002-)

involved_in tRNA processing

inferred from sequence or structural similarity with UniProtKB:Q9NWX6

(Gene Ontology Curators, 2002-)

inferred from biological aspect of ancestor with PANTHER:PTN000977687

(GO Reference Genome Project, 2011-)

Cellular Component (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

GUANYLYLTRANSFERASES

Protein Family (UniProt)

Belongs to the tRNA(His) guanylyltransferase family. (Q9V3N8)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

tRNA guanylyltransferase activity

a 5'-end ribonucleotide-tRNA(His) + ATP + GTP + H2O = a 5'-end phospho- guanosine-ribonucleotide-tRNA(His) + AMP + 2 diphosphate + H(+) (2.7.7.79)

RHEA 54564:

Summaries

Gene Group (FlyBase)

GUANYLYLTRANSFERASES -: Guanylyltransferases catalyse the transfer of a guanylyl group to an acceptor. Many eukaryotic guanylyl transferases are capping enzymes that catalyze the formation of the 5' cap in messenger RNAs. Others specifically add a guanine nucleotide to the transfer RNA for histidine, which is required for it to be aminoacylated by the histidine tRNA synthetase. Guanylyl transferases also exist for transferring guanosine nucleotides to sugar molecules, such as mannose and fucose. (Adapted from https://en.wikipedia.org/wiki/Guanylyltransferase.)

Protein Function (UniProtKB)

Adds a GMP to the 5'-end of tRNA(His) after transcription and RNase P cleavage.

(UniProt, Q9V3N8)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Thg for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9V3N8)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.45

Sequence Ontology: Class of Gene

gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Thg-RA

FBtr0080727

NM_135893

1149

286

Thg-RB

FBtr0303561

NM_001201886

1076

286

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Thg-PA

FBpp0080286

33.1

286

7.60

FBpp0292594

33.1

286

7.60

Q9V3N8

NP_001188815

ADV37065

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

286 aa isoforms: Thg-PA, Thg-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q9V3N8

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Thg using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

l(2)35Bc_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-1.17

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Thg in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Thg

View exonic expression by tissue for Dmel\Thg

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34876/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0283659

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0028925

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0283659

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 11 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 4 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Thg

Transgenic constructs containing regulatory region of Thg

Aberrations (Deficiencies and Duplications) ( 112 )

Inferred from experimentation ( 112 )

Gene not disrupted in

Df(2L)A47

(Gubb et al., 1990, Ashburner et al., 1982)

Df(2L)A63

(Ashburner et al., 1982)

Df(2L)A178

(Ashburner et al., 1982)

Df(2L)A217

(Ashburner et al., 1982)

Df(2L)A245

(Ashburner et al., 1982)

Df(2L)A260

(Ashburner et al., 1982)

Df(2L)A266

(Ashburner et al., 1982)

Df(2L)A379

(Ashburner et al., 1982)

Df(2L)A445

(Ashburner et al., 1982)

Df(2L)b75

(Gubb et al., 1990, Woodruff and Ashburner, 1979)

Df(2L)b84a7

(Furukawa et al., 1992)

Df(2L)el77

(Woodruff and Ashburner, 1979)

Df(2L)fn2

(Ashburner et al., 1982, Woodruff and Ashburner, 1979, O'Donnell et al., 1977)

Df(2L)fn3

(Ashburner, 1982, Ashburner et al., 1982, Woodruff and Ashburner, 1979, O'Donnell et al., 1977)

Df(2L)fn7

(Ashburner, 1982, Ashburner et al., 1982, Woodruff and Ashburner, 1979, O'Donnell et al., 1977)

Df(2L)fn36

(Ashburner et al., 1982, O'Donnell et al., 1977)

Df(2L)GT4

(Gubb et al., 1990)

Df(2L)rd9

(Furukawa et al., 1992)

Df(2L)Sco-1

(Ashburner, 1982)

Df(2L)TE35B-3

(Gubb et al., 1985)

Df(2L)TE35B-4

(Gubb et al., 1985)

Df(2L)TE35B-7

(Gubb et al., 1985)

Df(2L)TE35B-8

(Gubb et al., 1985)

Df(2L)TE35B-9

(Gubb et al., 1985)

Df(2L)TE35B-10

(Gubb et al., 1985)

Df(2L)TE35B-11

(Gubb et al., 1985)

Df(2L)TE35B-12

(Gubb et al., 1985)

Df(2L)TE35B-15

(Gubb et al., 1985)

Df(2L)TE35BC-1

(Gubb et al., 1984, Ashburner, 1982)

Df(2L)TE35BC-3

(Gubb et al., 1984, Ashburner, 1982)

Df(2L)TE35BC-24

(Iyengar et al., 1999, Gubb et al., 1984)

Df(2L)TE35D-2

(Furukawa et al., 1992)

Df(2L)TE35D-6

(Furukawa et al., 1992)

Df(2L)TE35D-13

(Furukawa et al., 1992)

Df(2L)TE35D-15

(Furukawa et al., 1992)

Df(2L)TE35D-18

(Furukawa et al., 1992)

Df(2L)W

(Woodruff and Ashburner, 1979)

In(2L)75c^LC158.1^R

(Woodruff and Ashburner, 1979)

T(2;3)Sco^rv7

(Woodruff and Ashburner, 1979)

T(Y;2)a15

(Davis et al., 1997)

T(Y;2)el⁴

(Davis et al., 1997)

T(Y;2)J165^DP58^P

(Woodruff and Ashburner, 1979)

T(Y;2)R15^DJ165^P

(Woodruff and Ashburner, 1979)

Gene disrupted in

Df(2L)64j

(Furukawa et al., 1992, Ashburner, 1982, Woodruff and Ashburner, 1979, O'Donnell et al., 1977)

Df(2L)A48

(Gubb et al., 1990, Ashburner et al., 1982)

Df(2L)A72

(Ashburner et al., 1982)

Df(2L)A215

(Ashburner et al., 1982)

Df(2L)A220

(Ashburner et al., 1982)

Df(2L)A246

(Ashburner et al., 1982)

Df(2L)A263

(Furukawa et al., 1992, Ashburner et al., 1982)

Df(2L)A264

(Ashburner et al., 1982)

Df(2L)A267

(Ashburner et al., 1982)

Df(2L)A376

(Ashburner, 1982, Ashburner et al., 1982)

Df(2L)A377

(Ashburner, 1982, Ashburner et al., 1982)

Df(2L)A400

(Ashburner, 1982, Ashburner et al., 1982)

Df(2L)ARR1

(Furukawa et al., 1992, Ashburner, 1982)

Df(2L)b84a3

(Furukawa et al., 1992)

Df(2L)b84h50

(Furukawa et al., 1992)

Df(2L)do1

(Ashburner, 1982)

Df(2L)el28

(Furukawa et al., 1992)

Df(2L)Exel7061

(Ryder, 2004.4.26)

Df(2L)fn1

(Ashburner et al., 1982, Woodruff and Ashburner, 1979, O'Donnell et al., 1977)

Df(2L)fn5

(Furukawa et al., 1992, Ashburner et al., 1982, O'Donnell et al., 1977)

Df(2L)fn12

(Ashburner et al., 1982, Woodruff and Ashburner, 1979)

Df(2L)fn15

(Ashburner et al., 1982, O'Donnell et al., 1977)

Df(2L)fn26

(Ashburner et al., 1982)

Df(2L)fn27

(Ashburner, 1982, Ashburner et al., 1982, O'Donnell et al., 1977)

Df(2L)fn30

(Ashburner et al., 1982, O'Donnell et al., 1977)

Df(2L)fn31

(Ashburner, 1982, Ashburner et al., 1982, O'Donnell et al., 1977)

Df(2L)GT5

(Gubb et al., 1990)

(Roote, 2002.2.18)

(Roote, 2000.6.13)

(Roote, 2000.6.13)

(Roote, 2000.6.13)

(Roote, 2000.6.13)

(Roote, 2000.6.13)

(Furukawa et al., 1992, Chia et al., 1985)

Df(2L)Sco^rv4

(Woodruff and Ashburner, 1979)

Df(2L)Sco^rv23

(Ashburner, 1982)

Df(2L)TE35B-1

(Gubb et al., 1985)

Df(2L)TE35B-2

(Gubb et al., 1985)

Df(2L)TE35B-5

(Gubb et al., 1985)

Df(2L)TE35B-6

(Gubb et al., 1985)

Df(2L)TE35BC-4

(Gubb et al., 1984, Ashburner, 1982)

Df(2L)TE35BC-6

(Gubb et al., 1984)

Df(2L)TE35BC-7

(Furukawa et al., 1992, Gubb et al., 1984)

Df(2L)TE35BC-8

(Gubb et al., 1984)

Df(2L)TE35BC-23

(Gubb et al., 1984)

Df(2L)TE35BC-28

(Furukawa et al., 1992, Gubb et al., 1984)

Df(2L)TE35BC-29

(Gubb et al., 1984)

Df(2L)TE35BC-31

(Gubb et al., 1984)

Df(2L)TE35BC-34

(Gubb et al., 1984)

Df(2L)TE35BC-35

(Gubb et al., 1984)

In(2L)75c

(Ashburner, 1982, Woodruff and Ashburner, 1979)

In(2L)C158^LSco^rv11R

(Ashburner and Harrington, 1984)

In(2L)C158^LSco^rv17R

(Ashburner and Harrington, 1984)

In(2L)TE35B-13

(Gubb et al., 1985)

In(2LR)noc^4LSco^rv9R

(Ashburner and Harrington, 1984)

In(2LR)TE35B-6^LSco^rv9R

(Ashburner and Harrington, 1984)

T(2;3)TE35B-2a

(Chia et al., 1985)

T(2;3)TE35B-3^DG16^P

(Chia et al., 1985)

T(Y;2)A80^DR15^P

(Woodruff and Ashburner, 1979)

Tp(2;2)A446

(Ashburner et al., 1982)

Ts(2Lt;3Lt)Mpe+Ts(2Rt;3Rt)G16

(Chia et al., 1985)

Ts(2Lt;3Lt)TE35B-4+Ts(2Rt;3Rt)Sco^rv13

(Chia et al., 1985, Ashburner and Harrington, 1984)

Ts(2Lt;3Lt)TE35BC-3+Ts(2Rt;3Rt)Sco^rv13

(Ashburner and Harrington, 1984)

Gene duplicated in

Dp(2;1)Sco^rv23

(Ashburner, 1982)

Dp(2;2)GYL

(Ashburner, 1982)

Dp(2;2)GYS

(Ashburner, 1982)

In(2L)C163.41^LC158^R

(Ashburner, 1982)

In(2L)Sco^rv17LC158^R

(Ashburner, 1982)

Gene not duplicated in

Dp(2;2)Adh3

(Ashburner, 1982)

Inferred from location ( 0 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

Thg^k08808

lethal, with Scer\GAL4^insc-Mz1407

Thg^GD11217

lethal | recessive

lethal - all die before end of P-stage, with Scer\GAL4^nub.PU

Thg^GD11217

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Thg^GD11217

viable, with Scer\GAL4^pnr-MD237

Thg^GD11217

viable, with Scer\GAL4^tin.CΔ4

Thg^GD11217

Other Phenotypes

Allele

abnormal neuroanatomy | larval stage, with Scer\GAL4^insc-Mz1407

Thg^GD11217

visible, with Scer\GAL4^pnr-MD237

Thg^GD11217

Phenotype manifest in

Allele

ganglion mother cell | larval stage, with Scer\GAL4^insc-Mz1407

Thg^GD11217

mesothoracic tergum, with Scer\GAL4^pnr-MD237

Thg^GD11217

trichogen cell, with Scer\GAL4^pnr-MD237

Thg^GD11217

wing, with Scer\GAL4^salm.EPv

Thg^GD11217

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (1)

Hsap\THG1L

14 of 14

Yes

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (1)

Rnor\Thg1l

14 of 14

Yes

Mus musculus (laboratory mouse) (1)

Mmus\Thg1l

13 of 14

Yes

Xenopus tropicalis (Western clawed frog) (2)

Xtro\thg1l

8 of 13

Yes

Xtro\clvs2

1 of 13

Danio rerio (Zebrafish) (1)

Drer\thg1l

14 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (0)

Anopheles gambiae (African malaria mosquito) (1)

Agam\AgaP_AGAP011752

12 of 12

Yes

Arabidopsis thaliana (thale-cress) (2)

Atha\AT2G31580

9 of 13

Yes

Atha\AT2G32320

9 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\THG1

13 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\SPCC63.07

12 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Thg. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (0)

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

THG1L; tRNA-histidine guanylyltransferase 1 like

model of autosomal recessive spinocerebellar ataxia 28

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

Thg^GD11217

ameliorates tauopathy

modeled by Hsap\MAPT^UAS.cUa

(Kim et al., 2021)

ameliorates Alzheimer's disease 1

modeled by Hsap\APP^{Aβ42.UAS.cBa}

(Kim et al., 2021)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

THG1L; tRNA-histidine guanylyltransferase 1 like

14 of 14

618802

SPINOCEREBELLAR ATAXIA, AUTOSOMAL RECESSIVE 28; SCAR28

autosomal recessive spinocerebellar ataxia 28

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Show neighbor-neighbor interactions:

Off

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

Select Layout:

Force Directed

Circle

Concentric

Legend:

Protein

RNA

Selected Interactor(s)

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Thg

two hybrid

(Hu et al., 2017.6.13)

Thg - snf

two hybrid

(Hu et al., 2017.6.13)

Summary of Genetic Interactions

esyN Network Diagram

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

esyN Network Key:

Suppression

Enhancement

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Smox

suppressible

Thg

(Hevia et al., 2017)

External Data

Linkouts

MIST (protein-protein) - An integrated Molecular Interaction Database

34876

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-50

Cytogenetic map

35B8-35B8

Sequence location

2L:15,008,489..15,009,724 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

35B8-35B8

Limits computationally determined from genome sequence between P{EP}elB^EP2039&P{PZ}osp^rJ571 and P{lacW}Su(H)^k07904

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

35B6-35B10

(determined by in situ hybridisation)

(Ashburner et al., 1999, Spradling et al., 1999)

35B6-35B10

(BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

2-50

(FlyBase, 2016)

2-47.8

(Comeron et al., 2012)

2-[50]

Left of (cM)

Su(H)

(Ashburner, 1982)

Rnmt

(Woodruff and Ashburner, 1979)

Right of (cM)

(Ashburner, 1982, Woodruff and Ashburner, 1979)

(Woodruff and Ashburner, 1979)

Notes

Complementation analysis establishes the following gene order (distal to proximal): dyn-p25, l(2)35Be, l(2)35Bc.

(Ashburner et al., 1999)

Excision of l(2)35Bc^k08808 indicates that the gene order is mol, dyn-p25, l(2)35Be, l(2)35Bc, l(2)35Bd.

(Ashburner et al., 1999.10.12)

Stocks and Reagents

Stocks (11)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

10810

y¹ w^67c23; P{lacW}Thg^k08808/CyO

30465

Adhⁿ¹ Thg⁴ PolD3^rd-s pr¹ cn¹ l(2)CA2^HG1/CyO

3772

b¹ Thg¹ pr¹/In(2L)Cy, In(2R)Cy, Duox^Cy Bl¹ cn²

Kyoto

101305

b¹ Thg⁶ Adhⁿ⁴ / CyO

111247

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{lacW}Thg^k08808 P{neoFRT}40A/CyO y⁺

101300

b¹ Adhⁿ² Thg² pr¹ cn¹ / CyO, Duox^Cy dpy^lv1 pr¹ cn²

NIG-Fly

M2L-2384

y¹ w¹¹¹⁸; Thg^SK3 P{neoFRT}40A/CyO-Tb

M2L-2385

y¹ w¹¹¹⁸; Thg^SK7 P{neoFRT}40A/CyO-Tb

2LG-1901

y² VhaAC39-1^cho-1 v¹; P{U6-Thg.NIG.sgRNA}attP40/CyO

VDRC

v105438

P{KK102440}VIE-260B

v26390

w¹¹¹⁸; P{GD11217}v26390

Genomic Clones (21)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (9)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

IP10135

Other clones

DPiM_IP10135

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0283659

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

tRNA-guanylyltransferase enzyme has been purified and characterised.

(Williams et al., 1990)

Relationship to Other Genes

Source for database merge of

Source for merge of: l(2)35Bc CG4103

(Huen and Roote, 2010.5.4)

Additional comments

"l(2)35Bc" probably corresponds to either " BG:DS00929.4 " or to " BG:DS00929.3 ".

(Ashburner et al., 1999)

" BG:DS00929.2 ", " BG:DS00929.3 " or " BG:DS00929.4 " may correspond to "l(2)35Bc".

(Ashburner et al., 1999.10.12)

" BG:DS00929.4 " may correspond to "l(2)35Bc".

(Ashburner et al., 1999.10.12)

The non-sequence localized gene 'tRNA-guanylyltransferase' (FBgn0023421), associated only with FBrf0052523, and the sequence localized gene 'l(2)35Bc/CG4103' (FBgn0261882) have been merged because the latter is the only CG-gene identified as a tRNA-guanylyltransferase (by protein domains or GO annotation).

(FlyBase, 1992-)

Nomenclature History

Source for database identify of

Source for identity of: BG:DS00929.4 CG4103

(Kodira, 1999.11)

Source for identity of: Thg l(2)35Bc

(FlyBase, 1992-)

Nomenclature comments

Etymology

Named 'Thg' based on the nomenclature used for this enzyme in other species.

(FlyBase, 1992-)

Synonyms and Secondary IDs (20)

Reported As

Symbol Synonym

BG:DS00929.4

(Giot et al., 2003, Ashburner et al., 1999, Ashburner et al., 1999, Ashburner et al., 1999.10.12)

(O'Donnell et al., 1977)

CG4103

(National Institute of Genetics Fly Stocks, 2022-, Kim et al., 2021, Hevia et al., 2017, Hosono et al., 2015, Japanese National Institute of Genetics, 2012.5.21, Huen and Roote, 2010.5.4, Keleman et al., 2009.8.5)

THG

(Hu et al., 2017.6.13)

Thg

br4

(Furukawa et al., 1992, Gubb et al., 1985, Ashburner and Harrington, 1984)

l(2)35Bc

(Huen and Roote, 2010.5.4)

l(2)br

(Ashburner, 1982)

l(2)br11

(Woodruff and Ashburner, 1979)

l(2)br13

(Woodruff and Ashburner, 1979)

l(2)br4

(Gubb et al., 1984, Ashburner et al., 1982, Ashburner et al., 1982, Woodruff and Ashburner, 1979)

l(2)k08808

tRNA-guanylyltransferase

Name Synonyms

lethal (2) 35Bc

lethal 11 in the black-reduced region

(Woodruff and Ashburner, 1979)

lethal 13 in the black-reduced region

(Woodruff and Ashburner, 1979)

lethal 4 in the black-reduced region

(Woodruff and Ashburner, 1979)

lethal group C1

(O'Donnell et al., 1977)

tRNA guanylyltransferase

(Williams et al., 1990)

tRNA-histidine guanylyltransferase

Secondary FlyBase IDs

FBgn0023421
FBgn0261882
FBgn0001973
FBgn0028925

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 28 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

34876

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9V3N8

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9V3N8

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9V3N8

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34876/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0283659

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0283659

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG4103

MARRVEL_MODEL - MARRVEL (model organism gene)

34876

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0283659

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

l(2)35Bc_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0028925

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0283659

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC014454

MIST (protein-protein) - An integrated Molecular Interaction Database

34876

References (56)

The following external sites may use different assemblies or annotations than FlyBase.

-1.17

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