R3D1, Loqs-PD, TRBP, R3D1-S, R3D1-L
a component of a functional pre-miRNA processing complex - stimulates and directs pre-microRNA processing activity - a Dicer-1 partner that enhances miRNA processing from hairpins with unstable structures at the dicing site
Please see the JBrowse view of Dmel\loqs for information on other features
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AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Gene model reviewed during 5.51
None of the polypeptides share 100% sequence identity.
Homodimer.
RNA-binding DRBM domains 1 and 2 bind dsRNA while DRBM 3 binds Dcr-1 (PubMed:29040648, PubMed:34590626, PubMed:36182693). The RNA-binding DRBM 1 and 2 domains are able to bind dsRNA independently (PubMed:34590626). The DRBM domains appears to bind to the RNA duplex and then slide along the siRNA helix to the ends of the siRNA (PubMed:29040648). The DRBMs display a preference for binding to the more thermodynamically stable ends of the siRNA which is enhanced by interaction with Dcr-2 (PubMed:29040648). This suggests that the domains may also function to pre-orientate the guide strand prior to loading into the (AGO2)-containing RNA-induced silencing complex (siRISC) (PubMed:29040648). Both of the DRBM 1 and 2 domains are required for optimal Dcr-2 mediated cleavage of 3' overhanging termini (3'ovr) dsRNAs, whereas only the DRBM 3 domain appears to be necessary for Dcr-2 mediated cleavage of blunt termini (BLT) dsRNAs (PubMed:28874570).
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\loqs using the Feature Mapper tool.
The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).
Eye-enriched transcripts determined by ratio of expression level in wild-type heads. versus expression level in so heads.
JBrowse - Visual display of RNA-Seq signals
View Dmel\loqs in JBrowse
2-48
2-43.8
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
monoclonal
polyclonal
New stable cell line derived from S2-unspecified : A stable line containing a knockout allele of loqs was created.
loqs acts primarily to process exogenous dsRNA.
Production of endogenous siRNAs depends on the loqs-PD isoform.
loqs is required intrinsically for germline stem cell maintenance in the ovary.
loqs is not required for repeat-associated small interfering RNA (rasiRNA) production
Source for identity of: loqs CG6866
The gene is named "loquacious" because loss of function de-silences both endogenous silencing and reporter expression in vivo.
Gene is designated as "loquacious" ("very talkative") as several types of silencing are lost in mutant flies.
