double-stranded RNA-binding protein - bridges the initiation and effector steps of the Drosophila RNAi pathway by facilitating siRNA passage from Dicer, which carrys out the initiation step, to RISC, which carrys out the effector step
Please see the JBrowse view of Dmel\r2d2 for information on other features
To submit a correction to a gene model please use the Contact FlyBase form
AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Gene model reviewed during 5.52
Low-frequency RNA-Seq exon junction(s) not annotated.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\r2d2 using the Feature Mapper tool.
The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).
JBrowse - Visual display of RNA-Seq signals
View Dmel\r2d2 in JBrowse2-28
2-25.1
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
monoclonal
polyclonal
r2d2 is essential for loading of exogenous siRNAs.
RNA is not methylated in the absence of r2d2.
r2d2 is required for transposon silencing in adult flies.
r2d2 is in the top 1% of fastest-evolving Drosophila genes.
r2d2 is not required for repeat-associated small interfering RNA (rasiRNA) production
The orientation of the Dcr-2/r2d2 protein heterodimer on the siRNA duplex determines which siRNA strand associates with the core RISC (RNA-induced silencing complex) protein AGO2. r2d2 protein binds the siRNA end with the greatest double-stranded character, thereby orienting the Dcr-2/r2d2 heterodimer on the siRNA duplex. Strong binding by the r2d2 protein requires a 5'-phosphate on the siRNA strand that is excluded from the RISC.
Dcr-2 protein alone efficiently cleaves dsRNA into siRNA. This enzymatic activity is not affected by association with r2d2 protein. Dcr-2 protein alone does not bind siRNA, but the r2d2/Dcr-2 protein complex binds siRNA and also facilitates the loading of siRNA onto the RNA-initiated silencing complex (RISC). This latter activity is dependent on the dsRNA-binding domains of the r2d2 protein.
Source for identity of: r2d2 CG7138
The protein is named "R2D2" because it contains two dsRNA-binding domains (R2) and is associated with Dcr-2 protein (D2).