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General Information
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| Term |
electrophoretic mobility shift assay |
ID (Ontology) |
MI:0413 (Molecular Interactions) |
| Definition |
This method proves the interaction between a nucleic acid and a protein partner. On the same electrophoresis gel 1 lane is loaded with a nucleic acid of known sequence, a second lane is loaded with the same nucleic acid together with a purified protein (or a protein mixture). The nucleic acid is often radio-labelled to enable visualisation by autoradiography. Comparison of the nucleic acid migration in the two lanes enables the retardation of the nucleic acid due to its interaction with a protein to be observed.[ PubMed:12169687 ] |
| Also Known As |
"band shift" ; "emsa" ; "Gel retardation assay" |
| Comment |
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Links to Extrernal Ontologies
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Annotations
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Records annotated with this term OR any of its CHILD TERMS
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Records annotated with this exact term (annotations to child terms are NOT included)
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No relevant records available
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Full annotation statements including this term (annotations to child terms are NOT included), and relevant FlyBase records
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| Full annotation statements | Relevant FlyBase reports |
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| Interaction Groups |
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electrophoretic mobility shift assay (all annotations which use CV term, excluding "NOT" statements) | 179 |
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Relationships