FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
loqs, mir-ban
FlyBase ID
FBig0000098350
Interaction Network
Interactions Browser links
loqs network
mir-ban network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0188216-5.coIP.NB
Reference
Saito et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
loqs 

Loqs

bait

FLAG tag

mir-ban 

ban

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

loqs coimmunoprecipitates mature miRNA forms of ban. Pre-miRNA ban coimmunoprecipitates with loqs if miRNA processing is inhibited by EDTA.

(Saito et al., 2005)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Saito et al., 2005)
FBrf0215456-10.coIP.NB
Reference
Miyoshi et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
loqs 

LOQS

bait
mir-ban 

bantam

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Anti-loqs immunoprecipitates both mature ban miRNA and ban pre-miRNA.

(Miyoshi et al., 2009)

Anti-loqs immunoprecipitates are not able to cleave target ban mRNA in vitro.

(Miyoshi et al., 2009)

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Miyoshi et al., 2009)
FBrf0237457-1a.FPS
Reference
Tants et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
loqs 

Loqs

bait

His tag, loqs-PD isoform

loqs 
loqs 
mir-ban 

ban

prey

fluorescein label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
loqs 
dsRBD1 domain
sufficient binding region
aa 129-211
loqs 
dsRBD2 domain
sufficient binding region
aa 245-322
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey oligo chemically synthesized and labeled in vitro.

(Tants et al., 2017)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)