FlyBase Gene Report: Dmel\blw

General Information

Symbol

Dmel\blw

Species

D. melanogaster

Name

bellwether

Annotation Symbol

CG3612

Feature Type

protein_coding_gene

FlyBase ID

FBgn0011211

Gene Model Status

Current

Stock Availability

15 publicly available

Enzyme Name (EC)

H(+)-transporting two-sector ATPase (7.1.2.2)

Gene Summary

bellwether (blw) encodes the alpha subunit of the mitochondrial F1F0 ATP synthase complex (complex V), the final enzyme of the oxidative phosphorylation pathway. Complex V catalyzes the synthesis of ATP from ADP and inorganic phosphate using the electrochemical proton gradient across the inner mitochondrial membrane. [Date last reviewed: 2018-09-13] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

ATP5A, ATPsyn-α, C-V-α, ATPsynα, Complex Vα

Key Links

Genomic Location

Cytogenetic map

59B2-59B2

Sequence location

2R:22,799,099..22,802,180 [+]

Recombination map

2-101

RefSeq locus

NT_033778 REGION:22799099..22802180

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (10 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables ADP binding

inferred from biological aspect of ancestor with PANTHER:PTN000389891

(GO Reference Genome Project, 2011-)

enables ATP binding

inferred from biological aspect of ancestor with PANTHER:PTN000389891

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR000194, InterPro:IPR020003

(InterPro Project Members, 2004-)

contributes_to proton-transporting ATP synthase activity, rotational mechanism

inferred from biological aspect of ancestor with PANTHER:PTN000389891

(GO Reference Genome Project, 2011-)

inferred from sequence or structural similarity with UniProtKB:P07251

(Gene Ontology Curators, 2002-)

enables proton-transporting ATP synthase activity, rotational mechanism

inferred from electronic annotation with InterPro:IPR005294

(InterPro Project Members, 2004-)

Biological Process (4 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

involved_in electron transport chain

inferred from direct assay

(Di Cara et al., 2013)

involved_in lipid storage

inferred from mutant phenotype

(Di Cara et al., 2013)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in proton motive force-driven ATP synthesis

inferred from biological aspect of ancestor with PANTHER:PTN000389891

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR000793, InterPro:IPR005294

(InterPro Project Members, 2004-)

inferred from sequence or structural similarity with SGD:S000000195

(Gene Ontology Curators, 2002-)

involved_in proton transmembrane transport

inferred from electronic annotation with InterPro:IPR004100, InterPro:IPR036121

(InterPro Project Members, 2004-)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in mitochondrion

inferred from high throughput direct assay

(Tan et al., 2009, Alonso et al., 2005)

inferred from direct assay

(Ge et al., 2019)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

part_of mitochondrial proton-transporting ATP synthase, catalytic core

inferred from biological aspect of ancestor with PANTHER:PTN000389894

(GO Reference Genome Project, 2011-)

inferred from sequence or structural similarity with UniProtKB:P07251

(Gene Ontology Curators, 2002-)

part_of proton-transporting ATP synthase complex, catalytic core F(1)

inferred from biological aspect of ancestor with PANTHER:PTN008732895

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR005294

(InterPro Project Members, 2004-)

Gene Group (FlyBase)

MITOCHONDRIAL COMPLEX V - ATP SYNTHASES

MITOCHONDRIAL COMPLEX V - TESTIS-SPECIFIC VARIANT

MITOCHONDRIAL COMPLEX V

Protein Family (UniProt)

Belongs to the ATPase alpha/beta chains family. (P35381)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

proton-transporting ATP synthase activity, rotational mechanism

ATP + 4 H(+)(in) + H2O = ADP + 5 H(+)(out) + phosphate (7.1.2.2)

RHEA 57722: RHEA 57720:

Summaries

Gene Snapshot

Gene Group (FlyBase)

MITOCHONDRIAL COMPLEX V - ATP SYNTHASES -: Mitochondrial ATP synthase catalyzes the formation of adenosine triphosphate (ATP) from adenosine diphosphate (ADP) and inorganic phosphate (Pi) at the expense of the transmembrane electrochemical proton gradient generated by the respiratory electron transport chain. The components of this complex are divided into two separable sectors, F1 and F0. The catalytic F1 sector is located in the matrix and comprises subunits alpha, beta, gamma, delta and epsilon with stoichiometry of 3:3:1:1:1 . Alternating alpha and beta subunits form a hexameric ring that constitute the reactive centers that undergo conformational changes upon rotation of subunit gamma, leading to synthesis of ATP. The catalytic sites are located in the beta subunits. (Adapted from PMID:30888962 and PMID:31418131.)
MITOCHONDRIAL COMPLEX V - TESTIS-SPECIFIC VARIANT -: Mitochondrial ATP synthase (Complex V) synthesizes ATP from ADP and inorganic phosphate using the energy provided by the proton electrochemical gradient (proton-motive force) across the inner mitochondrial membrane generated by electron transport complexes of the respiratory chain. It is an F-type ATPase and consists of two structural domains: the F1 sector, a soluble portion situated in the mitochondrial matrix, and the F0 sector, which spans the inner mitochondrial membrane. Protons pass from the inter membrane space to the matrix through F0, which transfers the energy created by the proton-motive force to F1 where ADP is phosphorylated to ATP using a rotational mechanism. (Adapted from PMID:23356252 and PMID:21874297).
MITOCHONDRIAL COMPLEX V -: Mitochondrial ATP synthase (Complex V) synthesizes ATP from ADP and inorganic phosphate using the energy provided by the proton electrochemical gradient (proton-motive force) across the inner mitochondrial membrane generated by electron transport complexes of the respiratory chain. It is an F-type ATPase and consists of two structural domains: the F1 sector, a soluble portion situated in the mitochondrial matrix, and the F0 sector, which spans the inner mitochondrial membrane. Protons pass from the inter membrane space to the matrix through F0, which transfers the energy created by the proton-motive force to F1 where ADP is phosphorylated to ATP using a rotational mechanism. (Adapted from PMID:23356252 and PMID:21874297).

Protein Function (UniProtKB)

Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites (By similarity).

(UniProt, P35381)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\blw for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P35381)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.52

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

blw-RA

FBtr0071883

NM_166554

2252

552

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

blw-PA

FBpp0071794

59.4

552

9.44

P35381

NP_726243

AAF46903

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c.

(UniProt, P35381)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

P35381

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\blw using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.80

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

No Assay Recorded

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

wing disc

(Santaren et al., 1993)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

adult stage | female

adult hemolymph

(Zeng et al., 2016)

day 5 of adulthood & male -- day 7 of adulthood & male

spermatozoon

(Wasbrough et al., 2010, Dorus et al., 2006)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Marker for

mitochondrion

(Li et al., 2016, Zhang et al., 2016)

Subcellular Localization

CV Term

Evidence

References

located_in mitochondrion

inferred from high throughput direct assay

(Tan et al., 2009, Alonso et al., 2005)

inferred from direct assay

(Ge et al., 2019)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\blw in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\blw

View exonic expression by tissue for Dmel\blw

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

37617/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0011211

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0011211

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0011211

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG3612

Flygut - An atlas of the Drosophila adult midgut

FBgn0011211

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 30 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 4 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of blw

Transgenic constructs containing regulatory region of blw

Aberrations (Deficiencies and Duplications) ( 7 )

Inferred from experimentation ( 7 )

Gene disrupted in

Df(2R)59AB

(Jacobs, 1998.9.29, Jacobs et al., 1998, BDGP Project Members, 1994-1999)

Df(2R)59AD

(Spradling et al., 1999, Jacobs et al., 1998, BDGP Project Members, 1994-1999)

Df(2R)599-5

(Jacobs et al., 1998)

Df(2R)BSC599

(Christensen et al., 2008.9.3)

Df(2R)d02208-f07572

(Ozdowski et al., 2009)

Gene not disrupted in

Df(2R)bw-D23

(BDGP Project Members, 1994-1999)

Df(2R)X58-12

(Jacobs et al., 1998)

Inferred from location ( 0 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^pnr-MD237

blw^GD11030

lethal | recessive

lethal - all die before end of larval stage | recessive

blw^k04409

lethal - all die during larval stage, with Scer\GAL4^Mef2.PR

blw^GD11030

partially lethal, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

blw^GD11030

partially lethal - majority die

blw⁰¹⁴¹⁰

partially lethal - majority die | pupal stage, with Scer\GAL4^pnr-MD237

blw^GD11030

viable

blw^EY08188

viable, with Scer\GAL4^tin.CΔ4

blw^GD11030

Sterility

Allele

fertile

blw^EY08188

male sterile, with Scer\GAL4^VP16.bam

blw^JF02896

male sterile (with blw¹)

blw^A2-2-27

male sterile (with blw^A2-2-27)

blw¹

male sterile (with Df(2R)59AB)

male sterile | recessive

Other Phenotypes

Allele

abnormal cell migration | embryonic stage, with Scer\GAL4^srp.Hemo

blw^GD11030

abnormal developmental rate | larval stage

blw^k04409

abnormal meiotic cell cycle | spermatogenesis, with Scer\GAL4^VP16.bam

blw^JF02896

abnormal mitotic cell cycle | cell autonomous | recessive

blw^k04409

abnormal mitotic cell cycle | recessive

blw^k04409

abnormal pain response | adult stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

blw^GD11030

abnormal size | adult stage, with Scer\GAL4^eya.enh1+E+2

blw^JF02896

decreased body size

blw^KG05893

decreased cell number | cell autonomous | larval stage | somatic clone

blw^k04409

decreased cell size | cell autonomous | somatic clone

blw^k04409

decreased fecundity | male, with Scer\GAL4^VP16.bam

blw^JF02896

decreased occurrence of cell division | larval stage

blw^k04409

increased cell number | spermatogenesis, with Scer\GAL4^VP16.bam

blw^JF02896

majority die during second instar larval stage

blw^KG05893

short lived, with Scer\GAL4^blw.AG

blw^UAS.cGa

short lived | female, with Scer\GAL4^blw.GT

blw^UAS.cGa

some die during larval stage | recessive

blw^k04409

visible, with Scer\GAL4^pnr-MD237

blw^GD11030

visible | adult stage, with Scer\GAL4^eya.enh1+E+2

blw^JF02896

Phenotype manifest in

Allele

adult heart, with Scer\GAL4^HCH.Hand

blw^GD11030

centrosome | spermatogenesis | increased number, with Scer\GAL4^VP16.bam

chromosome | spermatogenesis, with Scer\GAL4^VP16.bam

blw^JF02896

dorsal longitudinal indirect flight muscle cell | adult stage, with Scer\GAL4^Mhc.PW

blw^JF02896

embryonic/larval dorsal tracheal branch | larval stage, with Scer\GAL4^drm.7.1

blw^JF02896

embryonic/larval fat body

blw^KG05893

embryonic/larval plasmatocyte | embryonic stage, with Scer\GAL4^srp.Hemo

blw^GD11030

eye, with Scer\GAL4^eya.enh1+E+2

blw^JF02896

filamentous actin | adult stage, with Scer\GAL4^HCH.Hand

blw^GD11030

germ band, with Scer\GAL4^srp.Hemo

blw^GD11030

mesothoracic tergum, with Scer\GAL4^pnr-MD237

blw^GD11030

mitochondrion

blw^KG05893

mitochondrion | adult stage, with Scer\GAL4^Mhc.PW

blw^JF02896

spermatid, with Scer\GAL4^VP16.bam

blw^GD11030

spermatocyte, with Scer\GAL4^VP16.bam

vacuole | adult stage | male, with Scer\GAL4^VP16.bam

blw^GD11030

wing disc | cell autonomous | somatic clone

blw^k04409

yolk, with Scer\GAL4^srp.Hemo

blw^GD11030

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (5)

Hsap\ATP5F1A

14 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (4)

Rnor\Atp5f1a

14 of 14

Yes

Rnor\Atp5f1b

1 of 14

Rnor\Atp6v1b1

1 of 14

Rnor\Atp6v1b2

1 of 14

Mus musculus (laboratory mouse) (4)

Mmus\Atp5a1

13 of 14

Yes

Mmus\Atp5b

1 of 14

Mmus\Atp6v1b1

1 of 14

Mmus\Atp6v1b2

1 of 14

Xenopus tropicalis (Western clawed frog) (7)

Xtro\atp5f1a

7 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\st6gal2

1 of 13

Danio rerio (Zebrafish) (4)

Drer\atp5fa1

14 of 14

Yes

Drer\atp5f1b

1 of 14

Drer\atp6v1b2

1 of 14

Drer\atp6v1ba

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (6)

Cele\atp-1

13 of 14

Yes

Cele\T26E3.7

3 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (5)

Agam\AgaP_AGAP005134

12 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (11)

Atha\AT2G07698

13 of 13

Yes

Atha\atpA

7 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (4)

Scer\ATP1

13 of 13

Yes

Scer\ATP2

1 of 13

Scer\VMA1

1 of 13

Scer\VMA2

1 of 13

Schizosaccharomyces pombe (Fission yeast) (3)

Spom\atp1

12 of 12

Yes

Spom\atp2

1 of 12

Spom\vma2

1 of 12

Escherichia coli (enterobacterium) (3)

Ecol\atpA

10 of 11

Yes

Ecol\atpD

1 of 11

Ecol\fliI

1 of 11

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:blw. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (6)

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 3 )

Human Ortholog

Disease

Evidence

References

ATP5F1A; ATP synthase F1 subunit alpha

model of mitochondrial complex V (ATP synthase) deficiency nuclear type 4A

IEA

(FlyBase, 2019-)

model of mitochondrial complex V (ATP synthase) deficiency nuclear type 4B

IEA

(FlyBase, 2019-)

model of combined oxidative phosphorylation deficiency 22

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

ATP5F1A; ATP synthase F1 subunit alpha

14 of 14

164360

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Show neighbor-neighbor interactions:

Off

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

Select Layout:

Force Directed

Circle

Concentric

Legend:

Protein

RNA

Selected Interactor(s)

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

blw - ATPsynB

blue native page, peptide massfingerprinting, experimental knowledge based

(Rahman et al., 2014, Guruharsha et al., 2011)

blw - ATPsynCF6

experimental knowledge based, blue native page, peptide massfingerprinting

(Rahman et al., 2014, Guruharsha et al., 2011)

blw - ATPsynD

blue native page, peptide massfingerprinting, experimental knowledge based

(Rahman et al., 2014, Guruharsha et al., 2011)

blw - ATPsynE

blue native page, peptide massfingerprinting

(Rahman et al., 2014)

blw - ATPsynF

blue native page, peptide massfingerprinting, experimental knowledge based

(Rahman et al., 2014, Guruharsha et al., 2011)

blw - ATPsynG

blue native page, peptide massfingerprinting

(Rahman et al., 2014)

blw - ATPsynO

experimental knowledge based, blue native page, peptide massfingerprinting

(Rahman et al., 2014, Guruharsha et al., 2011)

blw - ATPsynβ

experimental knowledge based, blue native page, peptide massfingerprinting

(Rahman et al., 2014, Guruharsha et al., 2011)

blw - ATPsynγ

experimental knowledge based

(Guruharsha et al., 2011)

blw - ATPsynδ

blue native page, peptide massfingerprinting, experimental knowledge based

(Rahman et al., 2014, Guruharsha et al., 2011)

blw - Akt

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

blw - CG15098

experimental knowledge based

(Guruharsha et al., 2011)

blw - CG33303

experimental knowledge based

(Guruharsha et al., 2011)

blw - Chc

experimental knowledge based

(Guruharsha et al., 2011)

blw - Cpr78E

blue native page, peptide massfingerprinting

(Rahman et al., 2014)

blw - Gp93

experimental knowledge based

(Guruharsha et al., 2011)

blw - Hecw

pull down, Identification by mass spectrometry

(Fajner et al., 2021)

blw - Hsp83

experimental knowledge based

(Guruharsha et al., 2011)

blw - Invadolysin

anti tag coimmunoprecipitation, peptide massfingerprinting

(Di Cara et al., 2013)

blw - Ldh

experimental knowledge based

(Guruharsha et al., 2011)

blw - Mec2

experimental knowledge based

(Guruharsha et al., 2011)

blw - Rdh1

experimental knowledge based

(Guruharsha et al., 2011)

blw - SERCA

experimental knowledge based

(Guruharsha et al., 2011)

blw - Tapδ

experimental knowledge based

(Guruharsha et al., 2011)

blw - UQCR-C2

experimental knowledge based

(Guruharsha et al., 2011)

blw - cid

pull down, Identification by mass spectrometry, peptide array, anti tag western blot

(Collins et al., 2018)

blw - fax

experimental knowledge based

(Guruharsha et al., 2011)

blw - porin

experimental knowledge based

(Guruharsha et al., 2011)

blw - regucalcin

experimental knowledge based

(Guruharsha et al., 2011)

blw - rictor

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

blw - sima

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

blw - sun

blue native page, peptide massfingerprinting, experimental knowledge based

(Rahman et al., 2014, Guruharsha et al., 2011)

blw - wal

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

esyN Network Diagram

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

blw

suppressible

(Galloni and Edgar, 1999)

blw

suppressible

E2f1

(Galloni and Edgar, 1999)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Invadolysin

enhanceable

blw

(Di Cara et al., 2013)

peb

suppressible

blw

(Wilk et al., 2004)

trc

enhanceable

blw

(Hudson et al., 2023)

External Data

Subunit Structure (UniProtKB)

(UniProt, P35381 )

Linkouts

BioGRID - A database of protein and genetic interactions.

63229

DroID - A comprehensive database of gene and protein interactions.

FBgn0011211

MIST (protein-protein) - An integrated Molecular Interaction Database

37617

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

ATP biosynthesis

KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.

Formation of ATP by chemiosmotic coupling

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-101

Cytogenetic map

59B2-59B2

Sequence location

2R:22,799,099..22,802,180 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

59B2-59B2

; Limits computationally determined from genome sequence between P{EP}ppa^EP698&P{EP}EP2184 and P{PZ}blw⁰³⁹⁷²

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

59B1-59B2

(determined by in situ hybridisation)

(Spradling et al., 1999)

58F5-58F7

59A1--3 59B1--2

(BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

2-101

(FlyBase, 2016)

2-105.7

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (15)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

28059

y¹ v¹; P{TRiP.JF02896}attP2

11756

cn¹ P{PZ}blw¹/CyO; ry⁵⁰⁶

19869

y¹ w^67c23; P{EPgy2}blw^EY08188

10471

y¹ w^67c23; P{lacW}blw^k00212/CyO

14280

y¹; P{SUPor-P}blw^KG05893/CyO; ry⁵⁰⁶

Kyoto

104230

w^*; P{GawB}blw^NP2527 / CyO

112880

w^*; P{GawB}blw^NP2316 / CyO

113023

w^*; P{GawB}blw^NP2718 / CyO

101940

cn¹ P{PZ}blw¹ / CyO ; ry⁵⁰⁶

102309

y¹ w^67c23 ; P{lacW}blw^k04409 / CyO

VDRC

v34664

w¹¹¹⁸; P{GD11030}v34664

v34663

w¹¹¹⁸; P{GD11030}v34663/TM3

More stocks available...

Genomic Clones (16)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (929)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

HL08087

Other clones

DPiM_HL08087

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0011211

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0011211

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

blw mutants display phenotypes that affect lipid storage and mitochondrial electron transport chain activity resulting in a reduction in ATP production and an accumulation of reactive oxygen species.

(Di Cara et al., 2013)

Production of reactive oxygen species is enhanced in Invadolysin mutant mitochondria.

(Di Cara et al., 2013)

S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells.

(Stroschein-Stevenson et al., 2006)

RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R⁺ cells.

(Boutros et al., 2004)

Identification: One of a collection of genes identified with defective larval growth that extend larval life.

(Galloni and Edgar, 1999)

Postmeiotic differentiation defect.

(Castrillon et al., 1993)

Relationship to Other Genes

Source for database merge of

Source for merge of: blw ATPsyn-α

(Jacobs, 1998.9.29)

Source for merge of: blw l(2)03972 l(2)k00212 l(2)01410 ATPsyn-α

(Jacobs et al., 1998)

Additional comments

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (58)

Reported As

Symbol Synonym

A2(2nd)27

(Jacobs, 1998.5.21, Jacobs, 1998.5.21)

ATP syn-α

(Di Cara et al., 2013)

ATP5A

(Murakami et al., 2022, Sen and Cox, 2022, Wang et al., 2021, Cha et al., 2020, Chen et al., 2020, Jiang et al., 2020, Ge et al., 2019, Ikeda et al., 2019, Liu et al., 2019, Manola et al., 2019, Dragh et al., 2017, Yoon et al., 2017)

ATP5F1A

(Gumeni et al., 2019)

ATP5a

(Manola et al., 2021, Sieber et al., 2016, Kuang et al., 2014)

ATP5α

(Zhu et al., 2019, Chen et al., 2015)

ATPA

(Caggese et al., 2006)

ATPA_DROME

(Fukuyama et al., 2012)

ATPsyn-a

(Lotz et al., 2014)

ATPsyn-α

(Ren et al., 2023, Xu et al., 2019, Collins et al., 2018, Poernbacher and Vincent, 2018, DeVorkin et al., 2014, Yan et al., 2014, Spradling et al., 1999, Santaren et al., 1993)

ATPsynα

(van den Ameele and Brand, 2019, Porcelli and Marygold, 2014.9.16, Di Cara et al., 2013)

Atp5a

(Gumeni et al., 2023, Munafò et al., 2019)

Blw

(Huang et al., 2023, Ogneva et al., 2022, Usik et al., 2021, Andreazza et al., 2019, Gehrke et al., 2015, Anholt and Williams, 2010, Tiefenböck et al., 2010, Baltzer et al., 2009, Terhzaz et al., 2006)

C-V-α

(Tufi et al., 2014, de Castro et al., 2013, Pimenta de Castro et al., 2012)

C-Va

(Gehrke et al., 2015)

CVα

(Sing et al., 2014, Duboff et al., 2012)

CxV

(Humphrey et al., 2012)

blw

(Curcio et al., 2024, Everman et al., 2023, Hudson et al., 2023, Lee et al., 2023, Wong et al., 2023, Ding et al., 2022, O'Hanlon et al., 2022, Ogneva and Usik, 2021, Pennemann et al., 2021, Alvarado et al., 2020, Flatt, 2020, Ogneva et al., 2020, Parker et al., 2020, Vásquez-Procopio et al., 2020, Fowler et al., 2019, Mossman et al., 2019, Xu et al., 2019, Garcia et al., 2017, Li et al., 2016, Zhang et al., 2016, Dent et al., 2015, Wan et al., 2015, Chen et al., 2014, DeSalvo et al., 2014, Luck et al., 2014, Porcelli and Marygold, 2014.9.16, Yan et al., 2014, Di Cara et al., 2013, Kwon et al., 2013, Malone et al., 2012, Abruzzi et al., 2011, Friedman et al., 2011, Rees et al., 2011, Kallappagoudar et al., 2010, Monzo et al., 2010, Müller et al., 2010, Tiefenböck et al., 2010, Wasbrough et al., 2010, Wasbrough et al., 2010, Ozdowski et al., 2009, Tan et al., 2009, Chen et al., 2008, Christensen et al., 2008.9.3, FlyBase Curators, 2008-, Porcelli et al., 2007, Rathke et al., 2007, Xing et al., 2007, Beller et al., 2006, Caggese et al., 2006, Cermelli et al., 2006, Dorus et al., 2006, Liebl et al., 2006, Stroschein-Stevenson et al., 2006, Alonso et al., 2005, Tripoli et al., 2005, Stanyon et al., 2004, Wang et al., 2004)

dATP5A

(Xu et al., 2019)

l(2)01410

l(2)03972

l(2)59ABa

(Jacobs et al., 1998)

l(2)k00212

(Li et al., 2003)

ms(2)01504

ms(2)Pry58F

Name Synonyms

ATP SYNTHASE ALPHA

(Alonso et al., 2005)

ATP VDRC synthase F1F0

(Emtenani et al., 2022)

ATP synthase

(Anholt and Williams, 2010, Khanna et al., 2010)

ATP synthase Alpha chain

(Tripoli et al., 2005)

ATP synthase alpha chain

(Caggese et al., 2006)

ATP synthase alpha subunit of complex V

(Tiefenböck et al., 2010)

ATP synthase subunit alpha

(de Castro et al., 2013, Sun et al., 2011)

ATP synthase subunit α

(Pimenta de Castro et al., 2012, Shim et al., 2009)

ATP synthase subunits α

(Di Cara et al., 2013)

ATP synthase α

(Di Cara et al., 2013)

ATP synthase, subunit α

(Humphrey et al., 2012)

ATP synthase, α subunit

(Porcelli and Marygold, 2014.9.16)

ATP synthetase α chain

(Santaren et al., 1993)

BELLWETHER

(Alonso et al., 2005)

Bellweather

(Baltzer et al., 2009)

Bellwether

(Li et al., 2016, Robinson et al., 2010, Tiefenböck et al., 2010, Tan et al., 2009, Lindsay et al., 2008)

COMPLEX V: alpha chain

(Porcelli et al., 2007)

Complex V alpha

(Sen et al., 2013, Celotto et al., 2011)

Complex Va

(Monteiro et al., 2023)

Complex Vα

(Monteiro et al., 2023, Duboff et al., 2012, Ziviani et al., 2010)

F0/F1 ATP synthase Alpha

(Porcelli et al., 2007)

Mitochondrial ATP synthase subunit B

(Sun et al., 2007)

bellwether

(Gumeni et al., 2019, Sawyer et al., 2017, Porcelli and Marygold, 2014.9.16, Di Cara et al., 2013, Jaspers et al., 2012, Cammarato et al., 2011, Sun et al., 2011, Wasbrough et al., 2010, Rathke et al., 2007, Xing et al., 2007, Cermelli et al., 2006, Harbison et al., 2005)

colibri

(Galloni and Edgar, 1999)

complex Vα subunit

(Liu et al., 2011)

mitochondrial ATP synthase alpha subunit

(Jacobs, 1996.9.10)

mitochondrial ATP synthase subunit alpha of complex V

(Sun et al., 2011)

mitochondrial ATPase complex V-subunit α

(McQuibban et al., 2010)

α subunit of ATP synthase

(Khanna et al., 2010)

α subunit of the mitochondrial F1F0 ATPase

(Khanna et al., 2010)

α-ATP synthase

(Shim et al., 2009)

α-subunit of ATP synthase

(Baggio et al., 2014)

Secondary FlyBase IDs

FBgn0010487
FBgn0010561
FBgn0011298
FBgn0022274

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 58 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

37617

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P35381

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P35381

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P35381

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

37617/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0011211

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0011211

FlyMine - An integrated database for Drosophila genomics

FBgn0011211

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG3612

MARRVEL_MODEL - MARRVEL (model organism gene)

37617

Linkouts

BioGRID - A database of protein and genetic interactions.

63229

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0011211

DroID - A comprehensive database of gene and protein interactions.

FBgn0011211

DRSC - Results frm RNAi screens

FBgn0011211

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0011211

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0011211

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

ATP biosynthesis

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG3612

Flygut - An atlas of the Drosophila adult midgut

FBgn0011211

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0011211

KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

MIST (protein-protein) - An integrated Molecular Interaction Database

37617

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.

Formation of ATP by chemiosmotic coupling

References (215)

The following external sites may use different assemblies or annotations than FlyBase.

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