FB2024_02 , released April 23, 2024
Gene: Dmel\Invadolysin
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General Information
Symbol
Dmel\Invadolysin
Species
D. melanogaster
Name
Invadolysin
Annotation Symbol
CG3953
Feature Type
protein_coding_gene
FlyBase ID
FBgn0086359
Gene Model Status
Current
Stock Availability
10 publicly available
Gene Summary
Invadolysin (Invadolysin) encodes a metalloprotease that is required for mitosis, cell migration and mitochondrial function. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)
All Summaries
Also Known As

IX-14, l(3)IX-14

Key Links
Genomic Location
Cytogenetic map
85F14-85F15
Sequence location
Recombination map
3-49
RefSeq locus
NT_033777 REGION:10144668..10159134
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (19 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
enables peptidase activity
inferred from direct assay
(McHugh et al., 2004)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
enables metalloendopeptidase activity
inferred from electronic annotation with InterPro:IPR001577
(InterPro Project Members, 2004-)
enables peptidase activity
inferred from biological aspect of ancestor with PANTHER:PTN000101538
(GO Reference Genome Project, 2011-)
Biological Process (15 terms)
Terms Based on Experimental Evidence (13 terms)
CV Term
Evidence
References
involved_in brain development
inferred from mutant phenotype
(McHugh et al., 2004)
involved_in centrosome duplication
inferred from mutant phenotype
(McHugh et al., 2004)
involved_in chromatin remodeling
inferred from mutant phenotype
(McHugh et al., 2004)
involved_in electron transport chain
inferred from direct assay
(Di Cara et al., 2013)
involved_in germ cell migration
inferred from mutant phenotype
(McHugh et al., 2004)
involved_in gonad development
inferred from mutant phenotype
(McHugh et al., 2004)
involved_in imaginal disc development
inferred from mutant phenotype
(McHugh et al., 2004)
involved_in lipid storage
inferred from mutant phenotype
(Di Cara et al., 2013)
involved_in mitotic centrosome separation
inferred from mutant phenotype
(McHugh et al., 2004)
involved_in mitotic chromosome condensation
inferred from mutant phenotype
(McHugh et al., 2004)
involved_in mitotic spindle organization
inferred from mutant phenotype
(McHugh et al., 2004)
involved_in positive regulation of mitotic metaphase/anaphase transition
inferred from mutant phenotype
(McHugh et al., 2004)
involved_in regulation of mitochondrial membrane potential
inferred from mutant phenotype
(Di Cara et al., 2013)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
involved_in cell adhesion
inferred from electronic annotation with InterPro:IPR001577
(InterPro Project Members, 2004-)
involved_in proteolysis
inferred from electronic annotation with InterPro:IPR001577
(InterPro Project Members, 2004-)
Cellular Component (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
located_in cytoplasm
inferred from direct assay
(McHugh et al., 2004)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
is_active_in cytoplasm
inferred from biological aspect of ancestor with PANTHER:PTN000101538
(GO Reference Genome Project, 2011-)
located_in membrane
inferred from electronic annotation with InterPro:IPR001577
(InterPro Project Members, 2004-)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the peptidase M8 family. (Q9VH19)
Protein Signatures (InterPro)
Summaries
Gene Snapshot
Invadolysin (Invadolysin) encodes a metalloprotease that is required for mitosis, cell migration and mitochondrial function. [Date last reviewed: 2019-03-07]
Gene Group (FlyBase)
M8 METALLOENDOPEPTIDASES -
M8 metalloendopeptidase belong to MEROPS family M8, which contains leishmanolysin and its homologues. The tertiary structure of leishmanolysin places family M8 in the subclan of metzincins. (Adapted from FBrf0239469.)
Protein Function (UniProtKB)
Essential for the coordination of mitotic progression, and also plays a role in cell migration.
(UniProt, Q9VH19)
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Invadolysin for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9VH19)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.48

Sequence Ontology: Class of Gene
gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Invadolysin-RA
FBtr0082181
NM_143815
3610
683
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
Invadolysin-PA
FBpp0081659
77.0
683
7.51
Q9VH19
NP_652072
AAF54501
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Invadolysin using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.54

Transcript Expression
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in cytoplasm
inferred from direct assay
(McHugh et al., 2004)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Invadolysin in JBrowse
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 4-6
  • Stages(s) 9-10
  • Stages(s) 11-12
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 15 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 3 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Invadolysin
Transgenic constructs containing regulatory region of Invadolysin
Aberrations (Deficiencies and Duplications) ( 4 )
Inferred from experimentation ( 4 )
Gene disrupted in
Df(3R)by62
(McHugh, 1999.10.4)
Df(3R)Exel6265
(Ryder, 2004.4.26)
Gene not disrupted in
Df(3R)by10
(McHugh, 1999.10.4)
Df(3R)GB104
(McHugh, 1999.10.4)
Inferred from location ( 0 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal
lethal, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
lethal - all die before end of larval stage | recessive
lethal - all die during embryonic stage, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4pnr-MD237
viable, with Scer\GAL4tin.CΔ4
Other Phenotypes
Allele
abnormal behavior
abnormal mitotic cell cycle
abnormal mitotic cell cycle | recessive
abnormal size | larval stage
abnormal size | third instar larval stage
decreased occurrence of cell division | larval stage
majority die during larval stage
some die during larval stage | recessive
suppressor of variegation | dominant, with In(1)wm4
Phenotype manifest in
Allele
cell cycle
centrosome
condensed nuclear chromosome
embryonic/larval brain
embryonic/larval fat body
eye | temperature conditional, with Scer\GAL4GMR.PF
germline cell
gonad
imaginal disc
imaginal disc | larval stage
interommatidial bristle | temperature conditional, with Scer\GAL4GMR.PF
mitochondrion
neuroblast
ommatidium | temperature conditional, with Scer\GAL4GMR.PF
polytene chromosome
polytene chromosome band
polytene chromosome chromocenter
spindle
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (2)
Hsap\LMLN
14 of 14
Yes
Yes
Hsap\LMLN2
3 of 14
No
Yes
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (2)
Rnor\Lmln
14 of 14
Yes
Yes
Rnor\Lmln2
3 of 14
No
Yes
Mus musculus (laboratory mouse) (2)
Mmus\Lmln
14 of 14
Yes
Yes
Mmus\Gm29776
2 of 14
No
Yes
Xenopus tropicalis (Western clawed frog) (2)
Xtro\lmln
9 of 13
Yes
Yes
Xtro\lmln2
1 of 13
No
Yes
Danio rerio (Zebrafish) (2)
Drer\lmln
14 of 14
Yes
Yes
Drer\LOC100331300
3 of 14
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (2)
Cele\Y43F4A.1
13 of 14
Yes
Yes
Cele\T13B5.9
2 of 14
No
Yes
Anopheles gambiae (African malaria mosquito) (1)
Agam\AgaP_AGAP002955
12 of 12
Yes
Yes
Arabidopsis thaliana (thale-cress) (1)
Atha\AT5G42620
9 of 13
Yes
Yes
Saccharomyces cerevisiae (Brewer's yeast) (0)
Schizosaccharomyces pombe (Fission yeast) (0)
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:Invadolysin. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    LMLN; leishmanolysin like peptidase
    14 of 14
    609380
        CIROP; ciliated left-right organizer metallopeptidase
        3 of 14
        619703
          Functional Complementation Data
          Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
          Interactions
          Summary of Physical Interactions
          esyN Network Diagram
          Show neighbor-neighbor interactions:
          Show/hide secondary interactors 
          (data from AllianceMine provided by esyN)
          Select Layout:
          Legend:
          Protein
          RNA
          Selected Interactor(s)
          Interactions Browser
          View in Interactions Browser
          Please see the Physical Interaction reports below for full details
          MIST Molecular Interaction Search Tool
          View in MIST tool (external link)
          Please see the Physical Interaction reports below for full details
          protein-protein
          Physical Interaction
          Assay
          References
          Invadolysin - ATPsynD
          anti tag coimmunoprecipitation, peptide massfingerprinting
          (Di Cara et al., 2013)
          Invadolysin - ATPsynβ
          anti tag coimmunoprecipitation, peptide massfingerprinting
          (Di Cara et al., 2013)
          Invadolysin - blw
          anti tag coimmunoprecipitation, peptide massfingerprinting
          (Di Cara et al., 2013)
          Summary of Genetic Interactions
          esyN Network Diagram
          Show/hide secondary interactors 
          (data from AllianceMine provided by esyN)
          esyN Network Key:
          Suppression
          Enhancement
          View in Interactions Browser   View in MIST tool (external link)
          Please look at the allele data for full details of the genetic interactions
          Starting gene(s)
          Interaction type
          Interacting gene(s)
          Reference
          Invadolysin
          enhanceable
          blw
          (Di Cara et al., 2013)
          Invadolysin
          enhanceable
          SNF4Aγ
          (Di Cara et al., 2013)
          Starting gene(s)
          Interaction type
          Interacting gene(s)
          Reference
          brm
          suppressible
          Invadolysin
          (Tegeder et al., 2019)
          External Data
          Linkouts
          BioGRID - A database of protein and genetic interactions.
          DroID - A comprehensive database of gene and protein interactions.
          MIST (protein-protein) - An integrated Molecular Interaction Database
          Pathways
          Signaling Pathways (FlyBase)
          Metabolic Pathways
          External Data
          Linkouts
          Genomic Location and Detailed Mapping Data
          Chromosome (arm)
          3R
          Recombination map
          3-49
          Cytogenetic map
          85F14-85F15
          Sequence location
          FlyBase Computed Cytological Location
          Cytogenetic map
          Evidence for location
          85F14-85F15
          Limits computationally determined from genome sequence between P{EP}Fmr1EP3517&P{PZ}tws02414 and P{PZ}jumu06439&P{lacW}l(3)j8B6j8B6
          Experimentally Determined Cytological Location
          Cytogenetic map
          Notes
          References
          The original l(3)IX-141 allele was mapped to 85E10--85F16 by deficiency mapping. Subsequently, l(3)IX-144Y7 allele was mapped to 85F14--85F15 by mapping genomic DNA adjacent to the l(3)IX-144Y7 insertion to a P1 array.
          (McHugh et al., 2004)
          85F14-85F15
          Limits computationally determined from genome sequence between P{EP}Fmr1EP3517&P{PZ}tws02414 and P{PZ}jumu06439&P{lacW}l(3)j8B6j8B6
          (McHugh et al., 2004)
          Location based on hybridisation to a P1 clone.
          (McHugh, 1999.10.4)
          85F10-85F16
          (McHugh, 1999.10.4)
          Experimentally Determined Recombination Data
          Location

          3-49

          (FlyBase, 2016)

          3-46.6

          (Comeron et al., 2012)

          3-49.0

          (Gatti and Baker, 1989)
          Left of (cM)
          Right of (cM)
          Notes
          Stocks and Reagents
          Stocks (10)
          Genomic Clones (18)
           

          Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

          cDNA Clones (33)
           

          Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

          cDNA clones, fully sequenced
          BDGP DGC clones
          Other clones
            Drosophila Genomics Resource Center cDNA clones

            For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

            cDNA Clones, End Sequenced (ESTs)
            BDGP DGC clones
            Other clones
            RNAi and Array Information
            Linkouts
            DRSC - Results frm RNAi screens
            Antibody Information
            Laboratory Generated Antibodies
             
            Commercially Available Antibodies
             
            Cell Line Information
            Publicly Available Cell Lines
             
              Other Stable Cell Lines
               
                Other Comments

                Invadolysin mutants display phenotypes that affect lipid storage and mitochondrial electron transport chain activity resulting in a reduction in ATP production and an accumulation of reactive oxygen species.

                (Di Cara et al., 2013)

                Invadolysin mutant larvae show lower energetic status and higher oxidative stress than wild-type larvae.

                (Di Cara et al., 2013)

                Loss of Invadolysin affects mitochondrial function and reduces ATP production.

                (Di Cara et al., 2013)

                Production of reactive oxygen species is enhanced in Invadolysin mutant mitochondria.

                (Di Cara et al., 2013)

                Mutants show defects in mitotic chromosome condensation and mitotic spindle assembly and have dramatically increased levels of nuclear envelope proteins.

                (Yu and Heck, 2005)

                Homozygous larvae carrying mutations of l(3)IX-14 have poorly condensed chromosomes resulting in a fuzzy swollen appearance.

                (Krause and Heck, 1995)
                Relationship to Other Genes
                Source for database merge of

                Source for merge of: l(3)ds16 l(3)SG34

                (Shearn, 1995.5.3)

                Source for merge of: Invadolysin l(3)IX-14

                (Rao et al., 2007)

                Source for merge of: l(3)IX-14 CG3953

                (FlyBase, 1996-)
                Additional comments

                Source for merge of l(3)IX-14 CG3953 was sequence comparison ( date:001125 ).

                (FlyBase, 1996-)
                Nomenclature History
                Source for database identify of
                Nomenclature comments
                Etymology
                Synonyms and Secondary IDs (9)
                Datasets (0)
                Study focus (0)
                Experimental Role
                Project
                Project Type
                Title
                Study result (0)
                Result
                Result Type
                Title
                External Crossreferences and Linkouts ( 36 )
                Sequence Crossreferences
                NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
                GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
                UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
                Other crossreferences
                AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
                BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
                DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
                EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
                FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
                FlyMine - An integrated database for Drosophila genomics
                InterPro - A database of protein families, domains and functional sites
                KEGG Genes - Molecular building blocks of life in the genomic space.
                MARRVEL_MODEL - MARRVEL (model organism gene)
                Linkouts
                BioGRID - A database of protein and genetic interactions.
                Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
                DroID - A comprehensive database of gene and protein interactions.
                DRSC - Results frm RNAi screens
                Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
                FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
                FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
                Flygut - An atlas of the Drosophila adult midgut
                iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
                MIST (protein-protein) - An integrated Molecular Interaction Database
                References (66)