[85F5-85F5];[85F14-85F14];
A set of ~800 largely isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Designed to fill gaps in deletion coverage and breakpoint placement; also used to replace older available deficiencies that have not been molecularly mapped.
85F5;85F14
Breakpoint from FlyBase's release 5 sequence location of progenitor insertion.
1: PBac{RB}CG5359e03976
2: P{XP}Invadolysind08689
The presence of P+PBac{XP5.RB3}BSC621 was verified using the PCR methods and primers described in FBrf0175003.
The cytological breakpoints of Df(3R)BSC621 predicted from the Release 5 genomic coordinates of the insertion sites of the progenitor PBac{RB}CG5359e03976 and P{XP}Invadolysind08689 transposable elements are 85F5;85F14.