From: 	Kevin Cook <kcook@XXXX>
To: 	FlyBase-Cambridge <flybase-cambridgeXXXX>, Kim Cook <ruacookXXXX>, Stacey Christensen <sjchristXXXX>, kaufmanXXXX
Subject: 	Isolation and characterization of Df(3R)BSC621FlyBase-Cambridge, Kim Cook, Stacey Christensen, Thom Kaufman
Date: 	Mon, 29 Sep 2008  15:38:25  -0400  ( 20:38  BST)
Isolation and characterization of Df(3R)BSC621
Kim Cook, Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC621 was isolated as a FLP recombinase-induced recombination 
event involving PBac{RB}CG5359[e03976] and P{XP}Invadolysin[d08689]. 
The deletion was isolated as a chromosome lacking miniwhite markers 
in progeny of w[1118]; P{hs-hid}3, Dr[1]/TM6C, Sb[1] females crossed 
to P{hsFLP}1, y[1] w[1118]; 
PBac{RB}CG5359[e03976]/P{XP}Invadolysin[d08689] males. The males were 
heat shocked as larvae as described in Parks et al., Nature Genetics 
36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding 
and succeeding generations maintained the original genetic background 
of the Exelixis insertion stocks (Thibault et al., Nature Genetics 
36: 283-287, 2004; FBrf0175002). The recombination event generated 
the genetic element P+PBac{XP5.RB3}BSC621 from the segment of 
PBac{RB}CG5359[e03976] to the left of its FRT site and the segment of 
P{XP}Invadolysin[d08689] to the right of its FRT site. Its presence 
was verified using the PCR methods and primers described in Parks et 
al. The cytological breakpoints of Df(3R)BSC621 predicted from the 
Release 5 genomic coordinates of the transposable element insertions 
sites are 85F5;85F14. Df(3R)BSC621 failed to complement knk[1] and tws[02414].
__________________________________________________________
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology           http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX