ImpL3, Imp-L3, Ecdysone-inducible gene L3, dLdh, lincRNA.S4223
Please see the JBrowse view of Dmel\Ldh for information on other features
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AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.51
Gene model reviewed during 5.45
Gene model reviewed during 5.56
1.5 (northern blot)
332 (aa)
Homotetramer.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Ldh using the Feature Mapper tool.
The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).
Comment: anlage in statu nascendi
Ldh transcripts are abundant during late embryogenesis and in larval stages. Transcripts are present at low levels in pupae and increase again in adults. Ldh transcripts are detected in cultured imaginal discs and levels increase with exposure to 20-hydroxyecdysone. In situ hybridization shows that Ldh transcripts are abundantly expressed in somatic muscles from stage 13 on and are detected as early as stage 11. Staining is also observed in the posterior spiracles, pharyngeal muscles, and proventriculus by stage 15.
Levels of Ldh protein were measured by assaying for LDH activity. LDH activity is detected in cultured imaginal discs and is significantly higher in discs cultured with 20-hydroxyecdysone. LDH activity was found to be low in third instar larval leg discs but high in prepupal leg discs in the tarsal segments and the presumptive tibia. LDH activity localizes to somatic mesoderm and later to somatic muscle cells in embryos. Activity is also detected in the posterior spiracles, pharyngeal muscles, and proventriculus by stage 15.
JBrowse - Visual display of RNA-Seq signals
View Dmel\Ldh in JBrowse
3-16
3-14.5
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
ImpL3 encodes lactate dehydrogenase.
The ImpL3 lactate dehydrogenase product may have a similar function in imaginal disc morphogenesis as it does in muscle contraction, and maintains levels of metabolism required for active actin-myosin contraction in an environment characterized by reduced oxygen availability.
Temporal profile of gene expression is altered in Eip74EF mutant background.
Ecdysteroid-regulated gene.
Encodes a transcript associated with membrane-bound polysomes in imaginal discs and expressed only in response to 20-hydroxyecdysone (20 HOE). Expression studied both in vitro and in vivo. A 'late' gene involved in the eversion to the exterior of the elongated regions of discs by means of local changes in cell shape (Osterbur, Fristrom, Natzle, Tojo and Fristrom, 1988).
Source for merge of: ImpL3 CG10160
Source for identity of: Ldh ImpL3
The symbol/name of FBgn0001258 has been changed from 'Impl3/Ecdysone-inducible gene L3' to 'Ldh/Lactate dehydrogenase' to provide a more informative nomenclature that is consistent with usage in the literature.
