FB2024_03 , released June 25, 2024
Allele: Dmel\Brca247
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General Information
Symbol
Dmel\Brca247
Species
D. melanogaster
Name
FlyBase ID
FBal0282691
Feature type
allele
Associated gene
Dmel\Brca2
Associated Insertion(s)
P{?SUPor-P}Brca247
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
P-element activity
(Thomas et al., 2013)
Progenitor genotype
P{SUPor-P}KG02287
(Thomas et al., 2013)
Associated Insertion(s)
P{?SUPor-P}Brca247
Cytology
Description

2257bp deletion generated by imprecise excision of P{SUPor-P}KG02287. P-element sequences remain at the site of the excision.

(Thomas and McVey, 2014.3.1)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
2R:24,526,113..24,528,368 [+]
Comment:

Deletion resulting from the imprecise excision of P{SUPor-P}KG02287. Genomic sequences surrounding the deletion junction are CCTTCATGTT/CGTTGATACT. P element sequences remain at the site of the deletion.

(Thomas and McVey, 2014.3.1)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Brca247 mutants show defects in the DNA double-strand break repair as assessed by the P{hswa} male germline assay: the animals display no synthesis-dependent strand annealing (SDSA) repair events and a compensatory increase in the percentage of end joining repair events relative to wild-type.

      (Holsclaw and Sekelsky, 2017)

      Mutant flies are sensitive to camptothecin.

      (Thomas et al., 2013)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The defects in the DNA double-strand break repair (no synthesis-dependent strand annealing repair events and a compensatory increase in the percentage of end joining repair events) as assessed by the P{hswa} male germline assay characteristic for Brca247 are not modified by combination with Marcal1del/Marcal1kh1 alleles.

      (Holsclaw and Sekelsky, 2017)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Brca247
      (Carvajal-Garcia et al., 2021, Holsclaw and Sekelsky, 2017, Thomas and McVey, 2014.3.1)
      brca247
      (Kocak et al., 2019, Thomas et al., 2013)
      Name Synonyms
      Secondary FlyBase IDs
        References (5)