FlyBase Allele Report: Dmel\Marcal1[del]

General Information

Symbol

Dmel\Marcal1^del

Species

D. melanogaster

Name

FlyBase ID

FBal0276694

Feature type

allele

Associated gene

Dmel\Marcal1

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Baradaran-Heravi et al., 2012)

Progenitor genotype

P{SUPor-P}KG09850a

(Baradaran-Heravi et al., 2012)

Cytology

Description

Imprecise excision of the progenitor insertion has resulted in a 679bp deletion that extends from the middle of the first Marcal1 exon into the second intron.

(Baradaran-Heravi et al., 2012)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

delins

2L:4,956,255..4,956,933 [+]

Comment:

679 bp deletion from R5:2L:4956255..4956933 resulting from the imprecise excision of P{SUPor-P}Marcal1^KG09850, which extends from the end of the first exon to the second intron. There is also a 35 bp insertion (ATGATGAAATAACATCATTATATCGATTAACACAG) at the site.

Inserted_sequence:

ATGATGAAATAACATCATTATATCGATTAACACAG

(Morimoto and Boerkoel, 2014.3.07, Baradaran-Heravi et al., 2012)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal DNA repair | adult stage (with Marcal1^kh1)

(Holsclaw and Sekelsky, 2017)

short lived | heat sensitive

(Baradaran-Heravi et al., 2012)

Phenotype Manifest In

egg | heat sensitive

(Baradaran-Heravi et al., 2012)

Detailed Description

Statement

Reference

Marcal1^del/Marcal1^kh1 transheterozygous mutants show defects in the DNA double-strand break repair as assessed by the P{hsw^a} and P{wIw.FRT} male germline assay: the mutants display significantly reduced synthesis-dependent strand annealing (SDSA) repair (both in percentage of total progeny scored and in number of males with observable SDSA events in the progeny) as well as reduced single-strand annealing (SSA) capacity relative to wild-type controls.

(Holsclaw and Sekelsky, 2017)

Homozygous flies have no morphological differences to wild type and have a normal lifespan at 20[o]C.

Homozygotes are significantly less viable than wild type at 25[o]C (less than 75% viability) and at 30[o]C (less than 20% viability). The eggs laid by homozygous flies are smaller than normal at 25[o]C but not at 20[o]C.

(Baradaran-Heravi et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Marcal1^del/Marcal1^del is an enhancer of visible | adult stage phenotype of Scer\GAL4^ey.PU, brm^{K804R.UAS.Tag:HA}

(Morimoto et al., 2016)

Marcal1^del/Marcal1^del is an enhancer of abnormal size | adult stage phenotype of Scer\GAL4^ey.PU, brm^{K804R.UAS.Tag:HA}

(Morimoto et al., 2016)

NOT Enhancer of

Statement

Reference

Marcal1^del/Marcal1^kh1 is a non-enhancer of abnormal DNA repair | adult stage phenotype of Brca2⁴⁷

(Holsclaw and Sekelsky, 2017)

Suppressor of

Statement

Reference

Marcal1^del/Marcal1^del is a suppressor | partially of homeotic phenotype of Pc¹, ph-p[+]/ph-p⁴¹⁰

(Morimoto et al., 2016)

Marcal1[+]/Marcal1^del is a suppressor of homeotic phenotype of ph-p⁴¹⁰

(Morimoto et al., 2016)

Marcal1^del/Marcal1^del is a suppressor of homeotic phenotype of ph-p⁴¹⁰

(Morimoto et al., 2016)

Marcal1[+]/Marcal1^del is a suppressor of homeotic phenotype of Pc¹

(Morimoto et al., 2016)

Marcal1^del/Marcal1^del is a suppressor of homeotic phenotype of Pc¹

(Morimoto et al., 2016)

Marcal1[+]/Marcal1^del is a suppressor of homeotic phenotype of Psc¹

(Morimoto et al., 2016)

Marcal1[+]/Marcal1^del is a suppressor of homeotic phenotype of Scm^D1

(Morimoto et al., 2016)

NOT Suppressor of

Statement

Reference

Marcal1^del/Marcal1^kh1 is a non-suppressor of abnormal DNA repair | adult stage phenotype of Brca2⁴⁷

(Holsclaw and Sekelsky, 2017)

Other

Statement

Reference

Marcal1^del, Psc¹ has lethal phenotype

(Morimoto et al., 2016)

Marcal1^del, Scm^D1 has lethal phenotype

(Morimoto et al., 2016)

Marcal1^del, RpII215[+]/Polr2A⁴ has partially lethal - majority die | embryonic stage phenotype

(Baradaran-Heravi et al., 2012)

Marcal1^del, RpII215[+]/Polr2A³ has partially lethal - majority die | embryonic stage phenotype

(Baradaran-Heravi et al., 2012)

Marcal1^del, RpII215[+]/Polr2A⁸ has partially lethal - majority die | embryonic stage phenotype

(Baradaran-Heravi et al., 2012)

Marcal1^del, Polr2A^K1/RpII215[+] has partially lethal - majority die | embryonic stage phenotype

(Baradaran-Heravi et al., 2012)

Phenotype Manifest In

Enhancer of

Statement

Reference

Marcal1^del/Marcal1^del is an enhancer of eye phenotype of Scer\GAL4^ey.PU, brm^{K804R.UAS.Tag:HA}

(Morimoto et al., 2016)

Suppressor of

Statement

Reference

Marcal1[+]/Marcal1^del is a suppressor of sex comb phenotype of Scm^D1

(Morimoto et al., 2016)

Marcal1[+]/Marcal1^del is a suppressor of sex comb phenotype of ph-p⁴¹⁰

(Morimoto et al., 2016)

Marcal1^del/Marcal1^del is a suppressor of sex comb phenotype of ph-p⁴¹⁰

(Morimoto et al., 2016)

Marcal1^del/Marcal1^del is a suppressor | partially of wing phenotype of Pc¹, ph-p[+]/ph-p⁴¹⁰

(Morimoto et al., 2016)

Marcal1[+]/Marcal1^del is a suppressor of sex comb phenotype of Pc¹

(Morimoto et al., 2016)

Marcal1^del/Marcal1^del is a suppressor of sex comb phenotype of Pc¹

(Morimoto et al., 2016)

Marcal1[+]/Marcal1^del is a suppressor of sex comb phenotype of Psc¹

(Morimoto et al., 2016)

Other

Statement

Reference

Marcal1^del, RpII215[+]/Polr2A⁴ has egg phenotype

(Baradaran-Heravi et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

The defects in the DNA double-strand break repair (no synthesis-dependent strand annealing repair events and a compensatory increase in the percentage of end joining repair events) as assessed by the P{hsw^a} male germline assay characteristic for Brca2⁴⁷ are not modified by combination with Marcal1^del/Marcal1^kh1 alleles.

(Holsclaw and Sekelsky, 2017)

The homeotic transformation of the mesothoracic legs into prothoracic legs manifesting as extra sex combs observed in any of the following: ph-p⁴¹⁰/Y, Pc¹/+, Psc¹/+ and Scm^D1/+ males is partially suppressed by Marcal1^del heterozygosity. In ph-p⁴¹⁰/Y or Pc¹/+ males combination with Marcal1^del in homozygosity leads to a stronger rescue but in Psc¹/+ or Scm^D1/+ heterozygotes it leads to lethality. Similarly, the partial homeotic wing-to-haltere transformation characteristic for in ph-p⁴¹⁰/+;Pc¹/+ double heterozygotes is ameliorated by combination with Marcal1^del in homozygosity.

The small and rough eye phenotype seen in adult flies expressing brm^{K804R.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^ey.PU is exacerbated further by Marcal1^del homozygosity.

(Morimoto et al., 2016)

Marcal1^del/Marcal1^del RpII215⁴/+, Marcal1^del/Marcal1^del RpII215³/+, Marcal1^del/Marcal1^del RpII215⁸/+ and Marcal1^del/Marcal1^del RpII215^K1/+ embryos show a reduced hatching rate compared to controls at 20[o]C.

The eggs laid by Marcal1^del/Marcal1^del RpII215⁴/+ are smaller than normal.

(Baradaran-Heravi et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Marcal1^del

(Dewey et al., 2023, Holsclaw and Sekelsky, 2017, Morimoto et al., 2016, Morimoto et al., 2016, Morimoto and Boerkoel, 2014.3.07, Baradaran-Heravi et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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