FlyBase Allele Report: Dmel\PolD3[L2]

General Information

Symbol

Dmel\PolD3^L2

Species

D. melanogaster

Name

FlyBase ID

FBal0268415

Feature type

allele

Associated gene

Dmel\PolD3

Associated Insertion(s)

Carried in Construct

Also Known As

pol32^L2

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Kane et al., 2012)

Progenitor genotype

P{EPgy2}PolD3^EY15283

(Kane et al., 2012)

Cytology

Description

A 1.6-kb deletion within Pol32.

(Bhandari et al., 2019)

Imprecise excision of the progenitor insertion, resulting in a deletion that removes almost the entire Pol32 open reading frame.

(Kane et al., 2012)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal DNA repair

(Kocak et al., 2019, Kane et al., 2012)

chemical sensitive

(Kane et al., 2012)

female sterile

(Kane et al., 2012)

radiation sensitive

(Kane et al., 2012)

Phenotype Manifest In

Detailed Description

Statement

Reference

Pol32^L2 individuals show DNA repair defects in a P{w[α]} assay: decreased homologous recombination events despite of unchanged incomplete homologous recombination events that terminate via end joining, as compared to controls.

(Kocak et al., 2019)

Mutant larvae are extremely sensitive to methyl methanesulfonate, nitrogen mustard and ionising radiation. They are mildly sensitive to hydroxyurea, but are not sensitive to camptothecin.

Mutants show an approximately 70% decrease in full homologous recombination (HR) repair events in assays of excision and repair events of P{hsw^a}. Analysis of repair synthesis tract lengths suggests that this is due to a defect in primary repair synthesis.

(Kane et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

PolD3^L2 has abnormal DNA repair phenotype, enhanceable by Pif1¹⁶⁷

(Kocak et al., 2019)

PolD3^L2 has abnormal DNA repair phenotype, enhanceable by PolZ1^3B

(Kocak et al., 2019)

NOT Enhanced by

Statement

Reference

Brca2^KO, PolD3^L2 has lethal - all die before end of larval stage phenotype, non-enhanceable by spn-A³

(Kocak et al., 2019)

Brca2^KO, PolD3^L2 has lethal - all die before end of larval stage phenotype, non-enhanceable by mus81^Nhe

(Kocak et al., 2019)

Brca2^KO, PolD3^L2 has lethal - all die before end of larval stage phenotype, non-enhanceable by Pif1¹⁶⁷/mus81^Nhe

(Kocak et al., 2019)

NOT suppressed by

Statement

Reference

Brca2^KO, PolD3^L2 has lethal - all die before end of larval stage phenotype, non-suppressible by spn-A³

(Kocak et al., 2019)

Brca2^KO, PolD3^L2 has lethal - all die before end of larval stage phenotype, non-suppressible by mus81^Nhe

(Kocak et al., 2019)

Brca2^KO, PolD3^L2 has lethal - all die before end of larval stage phenotype, non-suppressible by Pif1¹⁶⁷/mus81^Nhe

(Kocak et al., 2019)

NOT Enhancer of

Statement

Reference

PolD3^L2 is a non-enhancer of lethal - all die before end of larval stage phenotype of Brca2⁴⁷, Pif1¹⁶⁷

(Kocak et al., 2019)

PolD3^L2 is a non-enhancer of lethal - all die before end of larval stage phenotype of Brca2^KO, Pif1¹⁶⁷

(Kocak et al., 2019)

spn-A³/PolD3^L2 is a non-enhancer of lethal - all die before end of larval stage phenotype of Brca2^KO, Pif1¹⁶⁷

(Kocak et al., 2019)

PolD3^L2/mus81^Nhe is a non-enhancer of lethal - all die before end of larval stage phenotype of Brca2^KO, Pif1¹⁶⁷

(Kocak et al., 2019)

NOT Suppressor of

Statement

Reference

PolD3^L2 is a non-suppressor of lethal - all die before end of larval stage phenotype of Brca2⁴⁷, Pif1¹⁶⁷

(Kocak et al., 2019)

PolD3^L2 is a non-suppressor of lethal - all die before end of larval stage phenotype of Brca2^KO, Pif1¹⁶⁷

(Kocak et al., 2019)

spn-A³/PolD3^L2 is a non-suppressor of lethal - all die before end of larval stage phenotype of Brca2^KO, Pif1¹⁶⁷

(Kocak et al., 2019)

PolD3^L2/mus81^Nhe is a non-suppressor of lethal - all die before end of larval stage phenotype of Brca2^KO, Pif1¹⁶⁷

(Kocak et al., 2019)

Other

Statement

Reference

PolD3^L2, mus81^Nhe has viable phenotype

(Kocak et al., 2019)

Brca2^KO, PolD3^L2 has lethal - all die before end of larval stage phenotype

(Kocak et al., 2019)

PolD3^L2, spn-A³ has viable phenotype

(Kocak et al., 2019)

Brca2⁴⁷, PolD3^L2 has lethal - all die before end of larval stage phenotype

(Kocak et al., 2019)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Both Pol32^L2, Pif1¹⁶⁷ and Pol32^L2, DNApol-ζ^3B double mutants show more pronounced DNA repair defects in a P{w[α]} assay than Pol32^L2 single mutants: more severely decreased homologous recombination events than Pol32^L2 and increased incomplete homologous recombination events that terminate via end joining, as compared to wild-type controls.

(Kocak et al., 2019)

Pol32^L2 mus205^3B double mutants show an approximately 70% decrease in full homologous recombination (HR) repair events in assays of excision and repair events of P{hsw^a} (similar to the reduction seen in Pol32^L2 single mutants). Repair synthesis tract lengths are reduced dramatically in aborted HR repair products compared to wild type, and this defect is more severe than that seen in Pol32^L2 single mutants.

Pol32^L2 DNApol-η¹² double mutants show a 60% decrease in full HR repair events in assays of excision and repair events of P{hsw^a}, but repair synthesis tract lengths of aborted HR products are increased dramatically over wild type.

(Kane et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

CG3975^L2

(Kane et al., 2012)

Pol32^L2

PolD3^L2

pol32^L2

(Bhandari et al., 2019, Kocak et al., 2019, Alexander et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

Open Close