FlyBase Allele Report: Dmel\Pif1[167]

General Information

Symbol

Dmel\Pif1¹⁶⁷

Species

D. melanogaster

Name

FlyBase ID

FBal0325580

Feature type

allele

Associated gene

Dmel\Pif1

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Alexander et al., 2016)

Progenitor genotype

P{EPgy2}Pif1^EY10295

(Alexander et al., 2016)

Cytology

Description

A 1.7-kb deletion within Pif1.

(Bhandari et al., 2019)

1695bp deletion that removes the Pif1 helicase domain. Generated by imprecise excision of P{EPgy2}CG3238^EY10295.

(Alexander et al., 2016)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate | embryonic stage | maternal effect

(Kocak et al., 2019)

abnormal mitotic cell cycle | embryonic stage | maternal effect

(Kocak et al., 2019)

chemical sensitive

(Kocak et al., 2019)

female sterile

(Kocak et al., 2019)

lethal | maternal effect | non-rescuable maternal effect

(Kocak et al., 2019)

majority die during embryonic stage | non-rescuable maternal effect

(Kocak et al., 2019)

Phenotype Manifest In

chromosome | embryonic stage | maternal effect

(Kocak et al., 2019)

nucleus | embryonic stage | maternal effect

(Kocak et al., 2019)

Detailed Description

Statement

Reference

Pif1¹⁶⁷ homozygotes show increased mortality to some chemicals that induce DNA replication stress: moderate sensitivity to hydroxyurea and mild sensitivity to methyl methanesulfonate and bleomycin, but no sensitivity to paraquat, topotecan or nitrogen mustard.

Embryos derived from Pif1¹⁶⁷ homozygous mothers rarely hatch and develop more slowly. During early development (1-2h of development at 25[o]C), these embryos show defective nuclear divisions: nuclear fallout (fully prevalent), chromosome clumping (highly prevalent), anaphase bridges and asynchronous divisions. However, there is no statistically significant increase in meiotic defects (i.e. X chromosome nondisjunction).

Pif1¹⁶⁷ individuals do not show significant DNA repair defects in P{w[α]} assays: slight decrease in homologous recombination events and unchanged incomplete homologous recombination events that terminate via end joining, as compared to controls.

(Kocak et al., 2019)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Brca2⁴⁷, Pif1¹⁶⁷ has lethal - all die before end of larval stage phenotype, non-enhanceable by PolD3^L2

(Kocak et al., 2019)

Brca2^KO, Pif1¹⁶⁷ has lethal - all die before end of larval stage phenotype, non-enhanceable by PolD3^L2

(Kocak et al., 2019)

Brca2^KO, Pif1¹⁶⁷ has lethal - all die before end of larval stage phenotype, non-enhanceable by spn-A³

(Kocak et al., 2019)

Brca2^KO, Pif1¹⁶⁷ has lethal - all die before end of larval stage phenotype, non-enhanceable by spn-A³/PolD3^L2

(Kocak et al., 2019)

Brca2^KO, Pif1¹⁶⁷ has lethal - all die before end of larval stage phenotype, non-enhanceable by mus81^Nhe

(Kocak et al., 2019)

Brca2^KO, Pif1¹⁶⁷ has lethal - all die before end of larval stage phenotype, non-enhanceable by PolD3^L2/mus81^Nhe

(Kocak et al., 2019)

NOT suppressed by

Statement

Reference

Brca2⁴⁷, Pif1¹⁶⁷ has lethal - all die before end of larval stage phenotype, non-suppressible by PolD3^L2

(Kocak et al., 2019)

Brca2^KO, Pif1¹⁶⁷ has lethal - all die before end of larval stage phenotype, non-suppressible by PolD3^L2

(Kocak et al., 2019)

Brca2^KO, Pif1¹⁶⁷ has lethal - all die before end of larval stage phenotype, non-suppressible by spn-A³

(Kocak et al., 2019)

Brca2^KO, Pif1¹⁶⁷ has lethal - all die before end of larval stage phenotype, non-suppressible by spn-A³/PolD3^L2

(Kocak et al., 2019)

Brca2^KO, Pif1¹⁶⁷ has lethal - all die before end of larval stage phenotype, non-suppressible by mus81^Nhe

(Kocak et al., 2019)

Brca2^KO, Pif1¹⁶⁷ has lethal - all die before end of larval stage phenotype, non-suppressible by PolD3^L2/mus81^Nhe

(Kocak et al., 2019)

Enhancer of

Statement

Reference

Pif1¹⁶⁷ is an enhancer of abnormal DNA repair phenotype of PolD3^L2

(Kocak et al., 2019)

NOT Enhancer of

Statement

Reference

Pif1¹⁶⁷/mus81^Nhe is a non-enhancer of lethal - all die before end of larval stage phenotype of Brca2^KO, PolD3^L2

(Kocak et al., 2019)

NOT Suppressor of

Statement

Reference

Pif1¹⁶⁷/mus81^Nhe is a non-suppressor of lethal - all die before end of larval stage phenotype of Brca2^KO, PolD3^L2

(Kocak et al., 2019)

Other

Statement

Reference

Pif1¹⁶⁷, mus81^Nhe has viable phenotype

(Kocak et al., 2019)

Brca2^KO, Pif1¹⁶⁷ has lethal - all die before end of larval stage phenotype

(Kocak et al., 2019)

Brca2⁴⁷, Pif1¹⁶⁷ has lethal - all die before end of larval stage phenotype

(Kocak et al., 2019)

Pif1¹⁶⁷, spn-A³ has viable phenotype

(Kocak et al., 2019)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Pol32^L2, Pif1¹⁶⁷ double mutants show more pronounced DNA repair defects in a P{w[α]} assay than Pol32^L2 single mutants: more severely decreased homologous recombination events than Pol32^L2 and increased incomplete homologous recombination events that terminate via end joining, as compared to wild-type controls.

Pif1¹⁶⁷, DNApol-ζ^3B individuals do not show significant DNA repair defects in P{w[α]} assays: slight decrease in homologous recombination events and unchanged incomplete homologous recombination events that terminate via end joining, as compared to controls.

(Kocak et al., 2019)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Pif1¹⁶⁷

pif1¹⁶⁷

(Bhandari et al., 2019, Kocak et al., 2019, Alexander et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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