FlyBase Allele Report: Dmel\Drp1[UAS.cDa]

General Information

Symbol

Dmel\Drp1^UAS.cDa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Deng

FlyBase ID

FBal0258603

Feature type

allele

Associated gene

Dmel\Drp1

Associated Insertion(s)

Carried in Construct

P{UAS-Drp1.D}

Also Known As

uas-drp1, DRP1^OE, UAS Drp1

Key Links

Transgenic product class

cDNA

(Deng et al., 2008)

wild_type

(Deng et al., 2008)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Deng et al., 2008)

wild_type

(Deng et al., 2008)

Mutagen

in vitro construct

(Deng et al., 2008)

Progenitor genotype

Carried in construct

P{UAS-Drp1.D}

Cytology

Description

UAS regulatory sequences drive expression of Drp1 cDNA clone AT04516.

(Deng et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Drp1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates neurodegenerative disease

modeled by trp^P365

(Huang et al., 2016)

exacerbates Huntington's disease

modeled by Hsap\HTT^Q93.ex1.UAS

(Khalil et al., 2015)

ameliorates frontotemporal dementia and/or amyotrophic lateral sclerosis 2

modeled by Chchd2^S81L.UAS

(Baek et al., 2021)

ameliorates hypoplastic left heart syndrome

modeled by Chchd3^HMC02376

(Birker et al., 2023)

ameliorates Parkinson's disease 6

modeled by Pink1^B9

(Li et al., 2023)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage, with Scer\GAL4^Mef2.PR

(Zhou et al., 2019)

increased cell number | third instar larval stage, with Scer\GAL4^e33C

(Ray et al., 2021)

lethal, with Scer\GAL4^Act5C.PI

(Edenharter et al., 2017)

Phenotype Manifest In

Detailed Description

Statement

Reference

The expression of Drp1^UAS.cDa under the control of Scer\GAL4^e33C results in the third instar larval lymph gland showing an increase in crystal cells in the primary lobe.

(Ray et al., 2021)

Expressing Drp1^UAS.cDa under the control of Scer\GAL4^Cg.PA leads to small and fragmented mitochondria in the early third instar larval fat body.

(Liu et al., 2020)

Expressing Drp1^UAS.cDa under the control of Scer\GAL4^Mef2.PR leads to a small but significant decrease in adult climbing ability, but does not have an obvious effect in the thorax muscles; muscle mitochondria also do not show obvious defects.

Expressing Drp1^UAS.cDa under the control of Scer\GAL4^Cg.PA does not seem to lead to mitochondrial defects in the third instar larval fat body (as assessed by MitoGFP).

(Zhou et al., 2019)

Expression of Drp1^UAS.cDa under the control of Scer\GAL4^Mef2.PR has no significant effect on larval locomotor activity or adult eclosion rate.

(Wang et al., 2016)

Flies expressing Drp1^Scer\UAS.cDa under the control of Scer\GAL4^Bx-MS1096 does not result in any wing defects.

(Ratnaparkhi, 2013)

Expression of Drp1^Scer\UAS.cDa under the control of Scer\GAL4^Mef2.PR results in mitochondrial fragmentation (they are smaller and rounder than normal) in the indirect flight muscles.

(Deng et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Drp1^UAS.cDa, Scer\GAL4^e33C has increased cell number | third instar larval stage phenotype, enhanceable by asrij⁹/asrij⁹

(Ray et al., 2021)

Enhancer of

Statement

Reference

Scer\GAL4^e33C/Drp1^UAS.cDa is an enhancer of increased cell number | third instar larval stage phenotype of asrij⁹

(Ray et al., 2021)

Drp1^UAS.cDa, Scer\GAL4^dome-PG14 is an enhancer of increased cell number | third instar larval stage phenotype of Scer\GAL4^dome-PG14, asrij^GD2167

(Ray et al., 2021)

Drp1^UAS.cDa/nanos^HMS00785, Scer\GAL4^Cg.PA is an enhancer of abnormal size | third instar larval stage phenotype of Dhpr^HMC05508, Scer\GAL4^Cg.PA

(Zhou et al., 2019)

Drp1^UAS.cDa, Scer\GAL4^elav.PU is an enhancer of short lived phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^elav.PU

(Khalil et al., 2015)

Suppressor of

Statement

Reference

Drp1^UAS.cDa, Scer\GAL4^Mef2.PR is a suppressor | partially of abnormal locomotor behavior | adult stage phenotype of Dhpr^HMC05508, Scer\GAL4^Mef2.PR

(Zhou et al., 2019)

Drp1^UAS.cDa, Scer\GAL4^Cg.PA is a suppressor of abnormal size | third instar larval stage phenotype of Dhpr^HMC05508, Scer\GAL4^Cg.PA

(Zhou et al., 2019)

Scer\GAL4^Cg.PA, Pu^HMC04085/Drp1^UAS.cDa is a suppressor | partially of abnormal size | third instar larval stage phenotype of Dhpr^HMC05508, Scer\GAL4^Cg.PA

(Zhou et al., 2019)

Drp1^UAS.cDa, Scer\GAL4^Mef2.PR is a suppressor of abnormal size | adult stage phenotype of Dhpr^HMC05508, Scer\GAL4^Mef2.PR

(Zhou et al., 2019)

Drp1^UAS.cDa/Scer\GAL4^GMR.PF is a suppressor of abnormal neuroanatomy | dominant | adult stage | progressive phenotype of trp^P365

(Huang et al., 2016)

Drp1^UAS.cDa, Scer\GAL4^Mef2.PR is a suppressor of abnormal locomotor behavior | third instar larval stage phenotype of Scer\GAL4^Mef2.PR, clu^GD13926

(Wang et al., 2016)

Drp1^UAS.cDa, Scer\GAL4^Mef2.PR is a suppressor | partially of partially lethal - majority die phenotype of Scer\GAL4^Mef2.PR, clu^GD13926

(Wang et al., 2016)

Drp1^UAS.cDa, Scer\GAL4^Act5C.PU is a suppressor | partially of partially lethal - majority die phenotype of PolG1^KK100485, Scer\GAL4^Act5C.PU

(Humphrey et al., 2012)

Drp1^UAS.cDa, Scer\GAL4^Act5C.PU is a suppressor | partially of some die during larval stage phenotype of PolG1^KK100485, Scer\GAL4^Act5C.PU

(Humphrey et al., 2012)

Drp1^UAS.cDa, Scer\GAL4^Act5C.PU is a suppressor | partially of some die during pupal stage phenotype of PolG1^KK100485, Scer\GAL4^Act5C.PU

(Humphrey et al., 2012)

Drp1^UAS.cDa/Scer\GAL4^Mef2.PR is a suppressor of visible phenotype of Pink1⁵

(Deng et al., 2008)

NOT Suppressor of

Statement

Reference

Drp1^UAS.cDa, Scer\GAL4^elav-C155 is a non-suppressor of abnormal locomotor behavior | adult stage phenotype of Scer\GAL4^elav-C155, fh^UAS.cLa

(Edenharter et al., 2017)

Other

Statement

Reference

Chchd2^S81L.UAS, Drp1^UAS.cDa, Scer\GAL4^GMR.PU has lethal phenotype

(Baek et al., 2021)

Phenotype Manifest In

Enhanced by

Statement

Reference

Drp1^UAS.cDa, Scer\GAL4^e33C has embryonic/larval lymph gland | third instar larval stage phenotype, enhanceable by asrij⁹/asrij⁹

(Ray et al., 2021)

Drp1^UAS.cDa, Scer\GAL4^e33C has embryonic/larval crystal cell | third instar larval stage | increased number phenotype, enhanceable by asrij⁹/asrij⁹

(Ray et al., 2021)

NOT Enhanced by

Statement

Reference

Drp1^UAS.cDa, Scer\GAL4^Cg.PA has mitochondrion | early third instar larval stage phenotype, non-enhanceable by Chchd2^8-8

(Liu et al., 2020)

Drp1^UAS.cDa, Scer\GAL4^Cg.PA has embryonic/larval fat body | early third instar larval stage phenotype, non-enhanceable by Chchd2^8-8

(Liu et al., 2020)

Enhancer of

Statement

Reference

Scer\GAL4^e33C/Drp1^UAS.cDa is an enhancer of embryonic/larval lymph gland | third instar larval stage phenotype of asrij⁹

(Ray et al., 2021)

Scer\GAL4^e33C/Drp1^UAS.cDa is an enhancer of primary lobe of embryonic/larval lymph gland | third instar larval stage phenotype of asrij⁹

(Ray et al., 2021)

Drp1^UAS.cDa, Scer\GAL4^dome-PG14 is an enhancer of embryonic/larval crystal cell | third instar larval stage | increased number phenotype of Scer\GAL4^dome-PG14, asrij^GD2167

(Ray et al., 2021)

Drp1^UAS.cDa, Scer\GAL4^dome-PG14 is an enhancer of embryonic/larval lymph gland | third instar larval stage phenotype of Scer\GAL4^dome-PG14, asrij^GD2167

(Ray et al., 2021)

Drp1^UAS.cDa/nanos^HMS00785, Scer\GAL4^Cg.PA is an enhancer of mitochondrion | third instar larval stage phenotype of Dhpr^HMC05508, Scer\GAL4^Cg.PA

(Zhou et al., 2019)

Drp1^UAS.cDa/nanos^HMS00785, Scer\GAL4^Cg.PA is an enhancer of embryonic/larval fat body | third instar larval stage phenotype of Dhpr^HMC05508, Scer\GAL4^Cg.PA

(Zhou et al., 2019)

Drp1^UAS.cDa, Scer\GAL4^Bx-MS1096 is an enhancer of wing phenotype of Scer\GAL4^Bx-MS1096, fog^UAS.cRa

(Ratnaparkhi, 2013)

Drp1^UAS.cDa, Scer\GAL4^Bx-MS1096 is an enhancer of wing | male limited phenotype of Scer\GAL4^Bx-MS1096, fog^UAS.cRa

(Ratnaparkhi, 2013)

NOT Enhancer of

Statement

Reference

Drp1^UAS.cDa, Scer\GAL4^Appl.G1a is a non-enhancer of mitochondrion | larval stage phenotype of Scer\GAL4^Appl.G1a, Zzzz\CAG^{127Q.UAS.Tag:HA}

(Swinter et al., 2023)

Drp1^UAS.cDa, Scer\GAL4^Appl.G1a is a non-enhancer of larval segmental nerve phenotype of Scer\GAL4^Appl.G1a, Zzzz\CAG^{127Q.UAS.Tag:HA}

(Swinter et al., 2023)

Suppressor of

Statement

Reference

Drp1^UAS.cDa/Scer\GAL4^Mhc.PU is a suppressor of indirect flight muscle cell phenotype of Pink1^B9

(Li et al., 2023)

Drp1^UAS.cDa/Scer\GAL4^Mhc.PU is a suppressor of mitochondrial crista | adult stage phenotype of Pink1^B9

(Li et al., 2023)

Drp1^UAS.cDa/Scer\GAL4^Mhc.PU is a suppressor of mitochondrion | adult stage phenotype of Pink1^B9

(Li et al., 2023)

Drp1^UAS.cDa/Scer\GAL4^Mhc.PU is a suppressor of mitochondrial membrane | adult stage phenotype of Pink1^B9

(Li et al., 2023)

Drp1^UAS.cDa, Scer\GAL4^Appl.G1a is a suppressor of mitochondrion | larval stage phenotype of Hsap\SNCA^UAS.cFa, Scer\GAL4^Appl.G1a

(Krzystek et al., 2021)

Scer\GAL4^e33C/Drp1^UAS.cDa is a suppressor | partially of mitochondrion | third instar larval stage phenotype of asrij⁹

(Ray et al., 2021)

Scer\GAL4^e33C/Drp1^UAS.cDa is a suppressor | partially of prohemocyte | third instar larval stage phenotype of asrij⁹

(Ray et al., 2021)

Scer\GAL4^e33C/Drp1^UAS.cDa is a suppressor | partially of embryonic/larval lymph gland | third instar larval stage phenotype of asrij⁹

(Ray et al., 2021)

Drp1^UAS.cDa, Scer\GAL4^Cg.PA is a suppressor of mitochondrion | third instar larval stage phenotype of Dhpr^HMC05508, Scer\GAL4^Cg.PA

(Zhou et al., 2019)

Drp1^UAS.cDa, Scer\GAL4^Cg.PA is a suppressor of embryonic/larval fat body | third instar larval stage phenotype of Dhpr^HMC05508, Scer\GAL4^Cg.PA

(Zhou et al., 2019)

Drp1^UAS.cDa, Scer\GAL4^Mef2.PR is a suppressor of adult somatic muscle cell of thorax | adult stage phenotype of Dhpr^HMC05508, Scer\GAL4^Mef2.PR

(Zhou et al., 2019)

Drp1^UAS.cDa, Scer\GAL4^Mef2.PR is a suppressor of mitochondrion | adult stage phenotype of Dhpr^HMC05508, Scer\GAL4^Mef2.PR

(Zhou et al., 2019)

Scer\GAL4^Cg.PA, Pu^HMC04085/Drp1^UAS.cDa is a suppressor | partially of mitochondrion | third instar larval stage phenotype of Dhpr^HMC05508, Scer\GAL4^Cg.PA

(Zhou et al., 2019)

Scer\GAL4^Cg.PA, Pu^HMC04085/Drp1^UAS.cDa is a suppressor | partially of embryonic/larval fat body | third instar larval stage phenotype of Dhpr^HMC05508, Scer\GAL4^Cg.PA

(Zhou et al., 2019)

Drp1^UAS.cDa/Scer\GAL4^GMR.PF is a suppressor of rhabdomere of eye photoreceptor cell | adult stage | progressive phenotype of trp^P365

(Huang et al., 2016)

Drp1^UAS.cDa, Scer\GAL4^Mef2.PR is a suppressor of mitochondrion | adult stage phenotype of Scer\GAL4^Mef2.PR, clu^GD13926

(Wang et al., 2016)

Drp1^UAS.cDa/Scer\GAL4^how-24B is a suppressor of mitochondrion phenotype of park²⁵

(Deng et al., 2008)

Drp1^UAS.cDa/Scer\GAL4^how-24B is a suppressor of indirect flight muscle cell phenotype of park²⁵

(Deng et al., 2008)

Drp1^UAS.cDa/Scer\GAL4^Mef2.PR is a suppressor of mitochondrion phenotype of Pink1⁵

(Deng et al., 2008)

Drp1^UAS.cDa/Scer\GAL4^Mef2.PR is a suppressor of wing phenotype of Pink1⁵

(Deng et al., 2008)

Drp1^UAS.cDa/Scer\GAL4^Mef2.PR is a suppressor of indirect flight muscle cell phenotype of Pink1⁵

(Deng et al., 2008)

NOT Suppressor of

Statement

Reference

Drp1^UAS.cDa, Scer\GAL4^Appl.G1a is a non-suppressor of mitochondrion | larval stage phenotype of Scer\GAL4^Appl.G1a, Zzzz\CAG^{127Q.UAS.Tag:HA}

(Swinter et al., 2023)

Drp1^UAS.cDa, Scer\GAL4^Appl.G1a is a non-suppressor of larval segmental nerve phenotype of Scer\GAL4^Appl.G1a, Zzzz\CAG^{127Q.UAS.Tag:HA}

(Swinter et al., 2023)

Drp1^UAS.cDa, Scer\GAL4^dome-PG14 is a non-suppressor of mitochondrion | third instar larval stage phenotype of Scer\GAL4^dome-PG14, asrij^GD2167

(Ray et al., 2021)

Drp1^UAS.cDa, Scer\GAL4^dome-PG14 is a non-suppressor of prohemocyte | third instar larval stage phenotype of Scer\GAL4^dome-PG14, asrij^GD2167

(Ray et al., 2021)

Drp1^UAS.cDa, Scer\GAL4^dome-PG14 is a non-suppressor of embryonic/larval lymph gland | third instar larval stage phenotype of Scer\GAL4^dome-PG14, asrij^GD2167

(Ray et al., 2021)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Drp1^Scer\UAS.cDa (positive regulator of mitofission) driven by Scer\GAL4^GMR.PF suppresses retinal degeneration (loss of rhabdomeres from photoreceptors) in 8 day old trp^P365/+ flies.

(Huang et al., 2016)

The larval locomotor deficit, very low adult eclosion rate as well as the mitochondrial morphology defects in adult indirect flight muscles (enlarged clustered mitochondria) characteristic for animals expressing clu^GD13926 under the control of Scer\GAL4^Mef2.PR can be suppressed to a varying degree by co-expression of Drp1^UAS.cDa.

(Wang et al., 2016)

Overexpression of Drp1^Scer\UAS.cDa under the control of Scer\GAL4^Bx-MS1096, enhances the wing phenotype of Scer\GAL4^Bx-MS1096>fog^Scer\UAS.cRa flies, with males appearing similar to Scer\GAL4^Bx-MS1096>fog^Scer\UAS.cRa males, with corresponding females showing strong wing defects ranging from relatively mild margin defects to strong wing defects that are similar to those in males.

(Ratnaparkhi, 2013)

Expression of Drp1^Scer\UAS.cDa partially suppresses the lethality seen when tam^KK100485 is expressed under the control of Scer\GAL4^Act5C.PU.

(Humphrey et al., 2012)

Expression of Drp1^Scer\UAS.cDa under the control of Scer\GAL4^elav.PU does not rescue mitochondrial morphology in Df(3L)Pmi-PGRP-LD flies.

(Rival et al., 2011)

Expression of Drp1^Scer\UAS.cDa under the control of Scer\GAL4^Mef2.PR suppresses the defects in mitochondrial morphology that are seen in the indirect flight muscles of Pink1⁵ mutants. The defects in wing posture and indirect flight muscle degeneration phenotypes are also suppressed.

Expression of Drp1^Scer\UAS.cDa under the control of Scer\GAL4^how-24B suppresses the defects in mitochondrial morphology that are seen in the indirect flight muscles of park²⁵ mutants. The indirect flight muscle degeneration phenotype is also suppressed.

(Deng et al., 2008)

Xenogenetic Interactions

Statement

Reference

Overexpression of Drp1^Scer\UAS.cDa in flies also expressing Scer\GAL4^elav.PU>Hsap\HTT^{Q93.ex1p.Scer\UAS} results in an about 23% further reduction in lifespan.

(Khalil et al., 2015)

Complementation and Rescue Data

Rescues

Drp1^UAS.cDa/Scer\GAL4^Cg.PA rescues Drp1^HMC03230

(Zhou et al., 2019)

Drp1^UAS.cDa/Scer\GAL4^elav.PU rescues Drp1²/Drp1¹

(Rival et al., 2011)

Comments

Expression of Drp1^Scer\UAS.cDa under the control of Scer\GAL4^elav.PU rescues mitochondrial morphology in Drp1¹/Drp1² flies.

(Rival et al., 2011)

Images (0)

Mutant