FlyBase Allele Report: Dmel\Dg[O55]

General Information

Symbol

Dmel\Dg^O55

Species

D. melanogaster

Name

FlyBase ID

FBal0218113

Feature type

allele

Associated gene

Dmel\Dg

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Christoforou et al., 2008)

Progenitor genotype

Cytology

Description

23bp deletion within exon 11 which is replaced with a 3bp insertion of unrelated sequence. This results in a frameshift that introduces a stop codon three amino acid residues after L653.

(Christoforou et al., 2008)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

delins

2R:16,084,473..16,084,495 [-]

Comment:

Inferred location of 23 bp deletion and 3 bp insertion in exon 11 of Dg-RA that leads to a frameshift after L653 of Dg-PA and a stop 3 amino acids downstream.

(Christoforou et al., 2008)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of muscular dystrophy

CEA with Dg^O86

(Carney et al., 2023, Yatsenko et al., 2021, Marrone et al., 2012, Kucherenko et al., 2011)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate

(Yatsenko et al., 2014)

abnormal locomotor behavior | temperature conditional (with Df(3R)Exel6184)

(Kucherenko et al., 2011)

abnormal locomotor behavior | temperature conditional (with Dg^O86)

(Kucherenko et al., 2011)

abnormal neuroanatomy

(Yatsenko et al., 2014)

partially lethal - majority die

(Yatsenko et al., 2014)

partially lethal - majority die (with Dg^O38)

(Yatsenko et al., 2014)

partially lethal - majority die (with Dg^O43)

(Yatsenko et al., 2014)

partially lethal - majority die (with Dg^O86)

(Yatsenko et al., 2014)

partially lethal - majority live

(Christoforou et al., 2008)

partially lethal - majority live (with Df(2R)ED2457)

(Christoforou et al., 2008)

partially lethal - majority live (with Dg^J43)

(Christoforou et al., 2008)

viable (with Dg²⁴⁸)

(Mirouse et al., 2009)

viable (with Dg³²³)

(Mirouse et al., 2009)

viable (with Dg^O38)

(Mirouse et al., 2009)

viable (with Dg^O43)

(Mirouse et al., 2009)

viable (with Dg^O86)

(Mirouse et al., 2009)

visible (with Dg^J43)

(Christoforou et al., 2008)

Phenotype Manifest In

crossvein (with Dg^J43)

(Christoforou et al., 2008)

embryonic/larval brain

(Yatsenko et al., 2014)

lamina plexus | third instar larval stage (with Dg^O86)

(Marrone et al., 2011)

neuroblast

(Yatsenko et al., 2014)

oocyte (with Dg^O43)

(Kucherenko et al., 2008)

oocyte (with Dg^O86)

(Kucherenko et al., 2008)

rhabdomere | adult stage (with Dg^O86)

(Marrone et al., 2011)

Detailed Description

Statement

Reference

Dg^O55/Dg^O86 show no significant changes in systolic diameter, diastolic diameter or fractional shortening compared to wild type.

(Taghli-Lamallem et al., 2014)

Homozygous Dg^O55 mutant flies exhibit a delay in pre-adult development and reduced viability.

The brains of Dg^O55 mutant larvae contain fewer M-phase neuroblasts per lobe than in controls. No increase in apoptotic cells is seen.

(Yatsenko et al., 2014)

Dg^O86/Dg^O55 mutant flies exhibit temperature-induced mobility defects.

Dg^O55/Df(3R)Exel6184 mutant flies exhibit temperature-induced mobility defects.

Increased indirect flight muscle degeneration is seen in Dg^O86/Dg^O55 mutant flies at 25[o]C and, as in wild type, the phenotype is increased ~2 fold by an increase in temperature to 33[o]C. On paraquat-containing food the ratio or degree of muscle degeneration is not increased in Dg^O86/Dg^O55 mutant flies in comparison to control. Unlike in wild type, animals exposure of Dg^O86/Dg^O55 mutants to lower temperature (18[o]C) causes the appearance of severe progressive degeneration, followed by focal muscle loss. At 25[o]C the phenotype does not become significantly more severe with age. Sugar-free food conditions promote severe muscle loss. Metabolic rate on sugar-free food is reduced compared to wild type controls.

(Kucherenko et al., 2011)

Dg^O86/Dg^O55 transheterozygous third instar larvae frequently show lamina plexus defects in the brain and the adults display significantly reduced rhabdomere length compared to wild-type.

(Marrone et al., 2011)

The Dg[-] progeny of Dg^O43/Dg^O86 females mated to Dg^O55/+ males are viable when grown without competition from heterozygous larvae.

The Dg[-] progeny of Dg^O55/Dg^O86 females mated to Dg^O38/+ males are viable when grown without competition from heterozygous larvae.

Dg^O86/Dg^O55 animals show reduced viability under normal culture conditions.

66% of eggs laid by Dg^O55/Dg^O86 females mated to wild-type males hatch.

79% of eggs laid by Dg^O43/Dg^O86 females mated to Dg^O55/+ males hatch.

82% of eggs laid by Dg^O55/Dg^O86 females mated to Dg^O38/+ males hatch.

(Mirouse et al., 2009)

Homozygotes show a posterior crossvein phenotype with 100% penetrance. More than 90% of wings show a "detached" phenotype (the crossvein is detached from both L4 and L5). A small fraction of wings with a "gapped" phenotype (the crossvein is detached from either vein L4 or L5, but not both) are seen.

Dg^O55/Df(2R)ED2457 animals show 65% viability compared to controls.

(Christoforou et al., 2008)

Dg^O86/Dg^O55 mutants are arrested at early stages of oogenesis and show mislocalisation of the oocyte.

Dg^O43/Dg^O55 mutants are arrested at early stages of oogenesis and show mislocalisation of the oocyte.

(Kucherenko et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Other

Statement

Reference

Df(3R)Exel6184/+, Dg^O55 has indirect flight muscle cell phenotype

(Kucherenko et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Dg Dys double heterozygous flies (Dg^O55/Df(3R)Exel6184) exhibit indirect flight muscle degeneration.

(Kucherenko et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Dg⁰⁵⁵

(Taghli-Lamallem et al., 2014)

Dg^O55

(Carney et al., 2023, Yatsenko et al., 2021, Yatsenko et al., 2014, Marrone et al., 2012, Kucherenko et al., 2011, Marrone et al., 2011, Mirouse et al., 2009, Mirouse et al., 2009, Christoforou et al., 2008, Kucherenko et al., 2008)

Name Synonyms

Secondary FlyBase IDs

References (11)

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