FlyBase Allele Report: Dmel\Dg[O43]

General Information

Symbol

Dmel\Dg^O43

Species

D. melanogaster

Name

FlyBase ID

FBal0218112

Feature type

allele

Associated gene

Dmel\Dg

Associated Insertion(s)

Carried in Construct

Also Known As

Dg⁰⁴³

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Christoforou et al., 2008)

Progenitor genotype

Cytology

Description

Nucleotide substitution: G2007A.

(Christoforou et al., 2008)

Amino acid replacement: W462term.

(Christoforou et al., 2008)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:16,085,069..16,085,069 [-]

Nucleotide change:

G16085069A

Reported nucleotide change:

G2007A

Amino acid change:

W462term | Dg-PA; W379term | Dg-PB; W644term | Dg-PC; W727term | Dg-PD

Reported amino acid change:

W462term

Comment:

TGG to TGA nonsense mutation in codon W462. Reported coordinates appear to be relative to GB:GH09323.

(Christoforou et al., 2008)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

partially lethal - majority die (with Dg^O55)

(Yatsenko et al., 2014)

partially lethal - majority live

(Christoforou et al., 2008)

partially lethal - majority live (with Df(2R)ED2457)

(Christoforou et al., 2008)

partially lethal - majority live (with Dg^J43)

(Christoforou et al., 2008)

viable (with Dg²⁴⁸)

(Mirouse et al., 2009)

viable (with Dg³²³)

(Mirouse et al., 2009)

viable (with Dg^O38)

(Mirouse et al., 2009)

viable (with Dg^O55)

(Mirouse et al., 2009)

viable (with Dg^O86)

(Mirouse et al., 2009)

visible (with Dg^J43)

(Christoforou et al., 2008)

Phenotype Manifest In

crossvein (with Dg^J43)

(Christoforou et al., 2008)

follicle cell (with Dg^O86)

(Mirouse et al., 2009)

oocyte (with Dg^O55)

(Kucherenko et al., 2008)

oocyte (with Dg^O86)

(Kucherenko et al., 2008)

stress fiber (with Dg^O86)

(Mirouse et al., 2009)

Detailed Description

Statement

Reference

The Dg[-] progeny of Dg^O38/Dg^O86 females mated to Dg^O43/+ males are viable when grown without competition from heterozygous larvae.

The Dg[-] progeny of Dg^O43/Dg^O86 females mated to Dg^O55/+ males are viable when grown without competition from heterozygous larvae.

Dg^O86/Dg^O43 animals show reduced viability under normal culture conditions.

67% of eggs laid by Dg^O43/Dg^O86 females mated to wild-type males hatch.

85% of eggs laid by Dg^O38/Dg^O86 females mated to Dg^O43/+ males hatch.

79% of eggs laid by Dg^O43/Dg^O86 females mated to Dg^O55/+ males hatch.

(Mirouse et al., 2009)

Females carrying homozygous germline clones produce eggs that are normal in shape.

Dg^O43/Dg^O86 follicle cells show a defect in the organisation of the actin stress fibres; the fibres are still properly aligned in mutant follicle cells, but they do not align along the dorsal-ventral axis, and they sometimes show a mixed orientation within a single cell.

Under normal food conditions, Dg^O43 mutant follicle cell clones have normal apical-basal polarity.

(Mirouse et al., 2009)

Homozygotes show a posterior crossvein phenotype with 100% penetrance. More than 90% of wings show a "detached" phenotype (the crossvein is detached from both L4 and L5). A small fraction of wings with a "gapped" phenotype (the crossvein is detached from either vein L4 or L5, but not both) are seen.

Dg^O43/Df(2R)ED2457 animals show 63% viability compared to controls.

(Christoforou et al., 2008)

Dg^O43/Dg^O55 mutants are arrested at early stages of oogenesis and show mislocalisation of the oocyte.

Dg^O86/Dg^O43 mutants are arrested at early stages of oogenesis and show mislocalisation of the oocyte.

(Kucherenko et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

63048

y¹ w^*; P{FRT(w^hs)}G13 sha¹ Dg^O43/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Dg⁰⁴³

(Villedieu et al., 2023, Koper et al., 2012)

Dg^O43

(Cerqueira Campos et al., 2020, Yatsenko et al., 2014, Mirouse et al., 2009, Christoforou et al., 2008, Kucherenko et al., 2008)

dg043

(Yatsenko et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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