UASt regulatory sequences drive expression of an inverted repeat.
Expressing Zip99CGD370 under the control of Scer\GAL4NP3084 is partially lethal (majority die) in flies fed an iron-depleted diet (but not under a regular or iron-rich diet).
Expressing Zip99CGD370 under the control of Scer\GAL4fkh.PH does not significantly affect the clearing of salivary gland tissue in pupae, as compared to controls.
Scer\GAL4NP3084-mediated expression of Zip99CGD370 results in whole body iron levels dropping by about 50%, while zinc levels stay unaltered.
Expression of Zip99CGD370 via Scer\GAL4da.PU results in developmental arrest in the pupal stage - only about 10% of animals eclose. Eclosion rates can be rescued via dietary iron supplementation, whereas zinc supplementation exacerbates the phenotype. The shortened lifespan of eclosed adults expressing Zip99CGD370 via Scer\GAL4da.PU is also partially rescued by dietary iron supplementation, but not zinc.
Expression of Zip99CGD370 under the control of Scer\GAL4GMR.PFa does not result in a mutant phenotype.
Expression of Zip99CGD370 under the control of Scer\GAL4pnr-MD237 does not result in a mutant phenotype.
Adults expressing Zip99CGD370 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) can show significantly reduced avoidance of noxious temperature (46[o]C) compared to control flies, depending on the particular P{GD370} insertion line used.
Adults expressing Zip99CGD370 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) show no significant change in the kinetics of high temperature (46[o]C) induced paralysis compared to control flies.
Scer\GAL4NP3084, Zip99CGD370 has partially lethal - majority die | nutrition conditional phenotype, enhanceable by Tsf1UAS.cXa/Scer\GAL4NP3084
Scer\GAL4NP3084, Zip99CGD370 has partially lethal - majority die | nutrition conditional phenotype, suppressible by Scer\GAL4NP3084/Tsf1KK103815
Scer\GAL4NP3084, Zip99CGD370 has partially lethal - majority die | nutrition conditional phenotype, suppressible | partially by Tsf1NIG.6186R/Scer\GAL4NP3084
Scer\GAL4unspecified, Zip99CGD370 has partially lethal - majority die phenotype, suppressible | partially by Hsap\SLC39A13UAS.cXa, Scer\GAL4unspecified
Scer\GAL4unspecified, Zip99CGD370 has short lived phenotype, suppressible | partially by Hsap\SLC39A13UAS.cXa, Scer\GAL4unspecified
Scer\GAL4unspecified, Zip99CGD370 has partially lethal - majority die phenotype, non-suppressible by Hsap\SLC39A7UAS.cXa, Scer\GAL4unspecified
Scer\GAL4unspecified, Zip99CGD370 has short lived phenotype, non-suppressible by Hsap\SLC39A7UAS.cXa, Scer\GAL4unspecified
Zip99CGD370/Scer\GAL4pnr-MD237 is an enhancer of visible phenotype of Scer\GAL4pnr-MD237, Zip42C.1UAS.EGFP, ZnT63CVDRC.cUa
Zip99CGD370/Scer\GAL4GMR.long is a suppressor | partially of visible | adult stage phenotype of Scer\GAL4GMR.long, Zip71BUAS.Tag:FLAG
Zip99CGD370/Scer\GAL4GMR.long is a suppressor | partially of abnormal eye color phenotype of Scer\GAL4GMR.long, Zip71BUAS.Tag:FLAG
Zip99CGD370/Scer\GAL4GMR.PFa is a suppressor of visible phenotype of Scer\GAL4GMR.PFa, Zip42C.1UAS.EGFP, ZnT63CVDRC.cUa
Zip99CGD370/Scer\GAL4GMR.long is a non-suppressor of visible | adult stage phenotype of Scer\GAL4GMR.long, ZnT86DUAS.cDa
Zip99CGD370/Scer\GAL4GMR.long is a non-suppressor of abnormal eye color phenotype of Scer\GAL4GMR.long, ZnT86DUAS.cDa
Zip99CGD370/Scer\GAL4GMR.long is a non-suppressor of visible | adult stage phenotype of Scer\GAL4GMR.long, ZnT86DUAS.EGFP
Zip99CGD370/Scer\GAL4GMR.long is a non-suppressor of abnormal eye color phenotype of Scer\GAL4GMR.long, ZnT86DUAS.EGFP
Scer\GAL4NP3084, Tsf1UAS.cXa, Zip99CGD370 has partially lethal - majority live phenotype
Scer\GAL4NP3084, Tsf1KK103815, Zip99CGD370 has viable phenotype
Scer\GAL4NP3084, Tsf1NIG.6186R, Zip99CGD370 has partially lethal - majority live | nutrition conditional phenotype
Zip99CGD370/Scer\GAL4pnr-MD237 is an enhancer of adult thorax phenotype of Scer\GAL4pnr-MD237, Zip42C.1UAS.EGFP, ZnT63CVDRC.cUa
Zip99CGD370/Scer\GAL4GMR.long is a suppressor | partially of eye phenotype of Scer\GAL4GMR.long, Zip71BUAS.Tag:FLAG
Zip99CGD370/Scer\GAL4GMR.PFa is a suppressor of eye phenotype of Scer\GAL4GMR.PFa, Zip42C.1UAS.EGFP, ZnT63CVDRC.cUa
Zip99CGD370/Scer\GAL4GMR.long is a non-suppressor of eye phenotype of Scer\GAL4GMR.long, ZnT86DUAS.cDa
Zip99CGD370/Scer\GAL4GMR.long is a non-suppressor of eye phenotype of Scer\GAL4GMR.long, ZnT86DUAS.EGFP
Co-expressing Tsf1KK103815 with Zip99CGD370 under the control of Scer\GAL4NP3084 results in a full rescue of lethality in flies fed an iron-depleted diet. Flies are viable also when fed a regular or iron-rich diet.
Co-expressing Tsf1NIG.6186R with Zip99CGD370 under the control of Scer\GAL4NP3084 results in a partial rescue of lethality in flies fed an iron-depleted diet.
The morphological and pigmentation defects in the adult eye seen upon expression of ZnT86DScer\UAS.cDa under the control of Scer\GAL4GMR.long do not change upon co-expression of Zip99CGD370 RNAi.
The morphological and pigmentation defects in the adult eye seen upon expression of ZnT86DScer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4GMR.long do not change upon co-expression of Zip99CGD370 RNAi.
The morphological and pigmentation defects in the adult eye seen upon expression of Zip71BScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4GMR.long are ameliorated by co-expression of Zip99CGD370 RNAi.
The mutant eye phenotype seen in flies co-expressing both ZnT63CVDRC.cUa and Zip42C.1Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4GMR.PFa is suppressed if the flies are also co-expressing Zip99CGD370.
The thorax defects caused by co-expression of ZnT63CVDRC.cUa and Zip42C.1Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4pnr-MD237 are enhanced if the flies are also co-expressing Zip99CGD370.