FlyBase Allele Report: Dmel\IKKε[DN.UAS]

General Information

Symbol

Dmel\IKKε^DN.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0194831

Feature type

allele

Associated gene

Dmel\IKKε

Associated Insertion(s)

Carried in Construct

P{UAS-IKKε.DN}

Key Links

Transgenic product class

missense_variant

(Oshima, 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Oshima, 2006)

Mutagen

in vitro construct

(Oshima, 2006)

Progenitor genotype

IKKε^UAS.cOa

(Oshima, 2006)

Carried in construct

P{UAS-IKKε.DN}

Cytology

Description

Amino acid replacement: K41A.

(Oshima, 2006)

The K41A mutation is within the catalytic center of the kinase domain.

(Oshima, 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

IKKε

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell shape, with Scer\GAL4^Act5C.PI

(Oshima et al., 2006)

abnormal neuroanatomy | pupal stage, with Scer\GAL4^ppk.PG

(Lee et al., 2009)

decreased occurrence of cell division | embryonic stage 13, with Scer\GAL4^sim.PS

(Barros and Bossing, 2021)

Phenotype Manifest In

actin filament bundle, with Scer\GAL4^ap-md544

(Otani et al., 2011)

arista lateral, with Scer\GAL4^ap-md544

(Oshima et al., 2006)

chaeta, with Scer\GAL4^ap-md544

(Otani et al., 2011)

dendrite & dorsal multidendritic neuron ddaC | pupal stage, with Scer\GAL4^ppk.PG

(Lee et al., 2009)

macrochaeta, with Scer\GAL4^ap-md544

(Oshima et al., 2006)

macrochaeta, with Scer\GAL4^{neur-GAL4-A101}

(Bitan et al., 2010)

microtubule, with Scer\GAL4^{neur-GAL4-A101}

(Bitan et al., 2010)

S2 cell-line & filamentous actin, with Scer\GAL4^Act5C.PI

(Oshima et al., 2006)

scutellar bristle, with Scer\GAL4^{neur-GAL4-A101}

(Bitan et al., 2010)

scutellar bristle, with Scer\GAL4^sca.PU

(Otani et al., 2011)

ventral midline of embryo | embryonic stage 13, with Scer\GAL4^sim.PS

(Barros and Bossing, 2021)

Detailed Description

Statement

Reference

The expression of IKKε^DN.UAS under the control of Scer\GAL4^sca-537.4 suppresses mitosis in the embryonic ventral midline, either under control conditions or upon the physical ablation of a section of the ventral midline.

(Barros and Bossing, 2021)

The number of actin bundles is significantly increased compared to wild type in the bristles of flies expressing ik2^DN.Scer\UAS under the control of Scer\GAL4^ap-md544.

(Otani et al., 2011)

Expression of ik2^DN.Scer\UAS under the control of Scer\GAL4^{neur-GAL4-A101} results in shorter and thicker anterior and posterior scutellar macrochaetae. Approximately 23% of these mutant bristles exhibit wild-type morphology, in which the shaft diameter is the widest at the bristle base and tapered toward the tip. In most of the mutant fly bristles, the widest shaft diameter is not found at the base of the bristle. Approximately 75% of the mutant bristles exhibit an altered growth direction that is not axially biased and 51% terminate in several minitips instead of a single tip. The middle third of the mutant bristles show moderately disorganized ridges that are shallower and more numerous than the wild-type bristle surface, which is characterized by straight ridges and valleys, suggesting that alterations in actin organization occurs in the mutants during the middle phase of bristle elongation.

Microtubule organisation and polarization is affected in bristles expressing ik2^DN.Scer\UAS under the control of Scer\GAL4^{neur-GAL4-A101}.

(Bitan et al., 2010)

The proximal dendrites remain intact for most ddaC neurons at 18 hours after puparium formation (APF) in animals expressing ik2^DN.Scer\UAS under the control of Scer\GAL4^ppk.PG, indicating a defect in dendritic severing during dendrite pruning (proximal dendrites can be seen disconnected from the ddaC soma at 5 hours APF in wild-type animals).

(Lee et al., 2009)

Expression of ik2^DN.Scer\UAS in S2 cells, under the control of Scer\GAL4^Act5C.PI, does not affect cell viability. However, these S2 cells show an increased likelihood to have a serrate or stellate morphology, instead of the typical round morphology. In the cells with the serrate morphology, there is a slight deceleration of the retrograde F actin flow.

Flies that express ik2^DN.Scer\UAS under the control of Scer\GAL4^ap-md544, show gnarled, forked macrochaetae. These flies also show abnormal aristae; the unicellular laterals are split at their tips or branched.

(Oshima et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

IKKε^DN.UAS, Scer\GAL4^sim.PS is an enhancer of increased occurrence of cell division | embryonic stage 13 phenotype of αTub67C^UASp.GFP, Scer\GAL4^sim.PS

(Barros and Bossing, 2021)

Phenotype Manifest In