FlyBase Allele Report: Dmel\Dark[RNAi.UAS.cLa]

General Information

Symbol

Dmel\Dark^RNAi.UAS.cLa

Species

D. melanogaster

Name

FlyBase ID

FBal0265673

Feature type

allele

Associated gene

Dmel\Dark

Associated Insertion(s)

Carried in Construct

P{UAS-Dark.RNAi.L}

Key Links

Transgenic product class

RNAi_reagent

(Leulier et al., 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Leulier et al., 2006)

Mutagen

in vitro construct

(Leulier et al., 2006, Oshima, 2006)

Progenitor genotype

Carried in construct

P{UAS-Dark.RNAi.L}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat of a fragment of Dark.

(Leulier et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Dark

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of pupal stage, with Scer\GAL4^αTub84B.PL

(Leulier et al., 2006)

some die during pupal stage, with Scer\GAL4^αTub84B.PL

(Leulier et al., 2006)

Phenotype Manifest In

chaeta | increased number, with Scer\GAL4^αTub84B.PL

(Leulier et al., 2006)

interommatidial precursor cell | pharate adult stage P9 | increased number, with Scer\GAL4^GMR.PF

(Leulier et al., 2006)

salivary gland | pupal stage, with Scer\GAL4^34B

(Leulier et al., 2006)

wing, with Scer\GAL4^αTub84B.PL

(Leulier et al., 2006)

Detailed Description

Statement

Reference

No apoptosis is observed in the dorsal compartment of the wing imaginal disc when Ark^{dsRNA.Scer\UAS.cLa} is expressed under the control of Scer\GAL4^ap-md544.

(Umemori et al., 2009)

Animals expressing Ark^{dsRNA.Scer\UAS.cLa} under the control of Scer\GAL4^αTub84B.PL have high levels of pupal lethality, and rare escapers display wing defects and supernumeral bristles.

Animals expressing Ark^{dsRNA.Scer\UAS.cLa} under the control of Scer\GAL4^GMR.PF have an aberrant number of pupal retinal lattice cells (68 hours after puparium formation).

Expression of Ark^{dsRNA.Scer\UAS.cLa} in the salivary glands under the control of Scer\GAL4^34B delays salivary gland histolysis during pupal development.

(Leulier et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^da.PU/Dark^RNAi.UAS.cLa is a suppressor of abnormal neuroanatomy | progressive phenotype of YME1L^del

(Qi et al., 2016)

Dark^RNAi.UAS.cLa/Scer\GAL4^GMR.PF is a suppressor of visible phenotype of hid^GMR.PG

(Leulier et al., 2006)

Dark^RNAi.UAS.cLa, Scer\GAL4^GMR.PF is a suppressor of visible phenotype of Diap1^RNAi.UAS.cLa, Scer\GAL4^GMR.PF

(Leulier et al., 2006)

Phenotype Manifest In

Enhancer of

Statement

Reference

Dark^RNAi.UAS.cLa, Scer\GAL4^ap-md544 is an enhancer of arista lateral phenotype of IKKε^DN.UAS, Scer\GAL4^ap-md544

(Oshima et al., 2006)

Suppressor of

Statement

Reference

Scer\GAL4^da.PU/Dark^RNAi.UAS.cLa is a suppressor of rhabdomere | progressive phenotype of YME1L^del

(Qi et al., 2016)

Dark^RNAi.UAS.cLa/Scer\GAL4^GMR.PF is a suppressor of eye phenotype of hid^GMR.PG

(Leulier et al., 2006)

Dark^RNAi.UAS.cLa, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Diap1^RNAi.UAS.cLa, Scer\GAL4^GMR.PF

(Leulier et al., 2006)

Dark^RNAi.UAS.cLa, Scer\GAL4^GMR.PF is a suppressor of interommatidial bristle phenotype of Diap1^RNAi.UAS.cLa, Scer\GAL4^GMR.PF

(Leulier et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Dark^{dsRNA.Scer\UAS.cLa} under the control of Scer\GAL4^da.PU suppresses the age-dependent loss of rhabdomeres seen in YME1L^del homozygotes.

Expression of Dark^{dsRNA.Scer\UAS.cLa} under the control of Scer\GAL4^da.PU partially suppresses the age-dependent locomotor defect seen in YME1L^del homozygotes.

(Qi et al., 2016)

The Scer\GAL4^ap-md544>ik2^DN.Scer\UAS arista lateral phenotype is enhanced by RNAi of Ark, generated by expression of Ark^{dsRNA.Scer\UAS}.

(Oshima et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Carried in a plasmid and transfected into S2 cells. Transcription from the plasmid should produce Ark dsRNA, resulting in dsRNA interference (RNAi) of the Ark gene.

(Oshima et al., 2006)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

Ark^{dsRNA.Scer\UAS.cLa}

Ark^{dsRNA.Scer\UAS}

Dark^RNAi.UAS.cLa

Dark^{dsRNA.Scer\UAS.cLa}

Dark^{dsRNA.UAS.cLa}

dArk^IR

(Qi et al., 2016)

Name Synonyms

Secondary FlyBase IDs

FBal0244094
FBal0194835

References (6)

Report Sections

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