FlyBase Allele Report: Dmel\lncRNA:Hsrω[EP3037]

General Information

Symbol

Dmel\lncRNA:Hsrω^EP3037

Species

D. melanogaster

Name

FlyBase ID

FBal0194559

Feature type

allele

Associated gene

Dmel\lncRNA:Hsrω

Associated Insertion(s)

P{EP}lncRNA:Hsrω^EP3037

Carried in Construct

Also Known As

EP3037

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Associated Insertion(s)

P{EP}lncRNA:Hsrω^EP3037

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates neurodegenerative disease

modeled by Zzzz\CAG^{127Q.UAS.Tag:HA}

(Mallik and Lakhotia, 2009, Sengupta and Lakhotia, 2006)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of a single copy of lncRNA:Hsrω^EP3037 under the control of Scer\GAL4^dpp.blk1 causes a mild disruption of photoreceptor cells; expression of two copies causes high mortality during development and severe disruption of photoreceptors.

Expression of a single copy of lncRNA:Hsrω^EP3037 under the control of Scer\GAL4^elav-C155 causes an increase in pupal lethality with no effect on lifespan.

Expression of a single copy of lncRNA:Hsrω^EP3037 under the control of Scer\GAL4^ptc-559.1 causes changes in scutellar bristle polarity; expression of two copies causes extra or missing scutellar bristles.

Expression of a single copy of lncRNA:Hsrω^EP3037 under the control of Scer\GAL4^C805 causes partial lethality during the pupal stage, with survivors being very weak; expression of two copies additionally causes wing blistering, male sterility and sex comb malformation, with a reduction in the number of sex comb teeth.

Expression of lncRNA:Hsrω^EP3037 under the control of Scer\GAL4^c825 does not result in sex comb defects.

Expression of lncRNA:Hsrω^EP3037 under the control of Scer\GAL4^GMR.PU results in complete lethality during early stages of development.

Expression of lncRNA:Hsrω^EP3037 under the control of Scer\GAL4^Act5C.PI causes a majority of animals to die before adult stage; pupal mortality is greatly increased and fertility is reduced in flies with two copies of lncRNA:Hsrω^EP3037; the gender ratio of survivors is skewed in favor of females; flies with a single copy of lncRNA:Hsrω^EP3037 are fertile, but have reduced fecundity. Expression of lncRNA:Hsrω^EP3037 under the control of Scer\GAL4^Act5C.PI also results in fewer, larger omega speckles in the nucleus.

(Mallik and Lakhotia, 2011)

Expression of one copy of Hsrω^EP3037 using Scer\GAL4^GMR.PF at 25[o]C causes disruption in the organization of ommatidial units in larval eye discs.

(Mallik and Lakhotia, 2010)

Eyes of Hsrω^EP3037 homozygous flies show disorganisation of ommatidial units.

(Sengupta and Lakhotia, 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, Zzzz\CAG^{127Q.UAS.Tag:HA}, lncRNA:Hsrω^EP3037 has abnormal eye color phenotype, suppressible | partially by lncRNA:Hsrω^RNAi.Sym.UAS/Scer\GAL4^GMR.PF

(Mallik and Lakhotia, 2009)

Enhancer of

Statement

Reference

lncRNA:Hsrω^EP3037/ lncRNA:Hsromega [+] is an enhancer of visible | adult stage phenotype of Scer\GAL4^GMR.PU

(Mallik and Lakhotia, 2011)

lncRNA:Hsrω^EP3037, Scer\GAL4^GMR.PF is an enhancer of partially lethal - majority die | pupal stage phenotype of Df(3R)Hrb87F/+, Scer\GAL4^GMR.PF, Zzzz\CAG^{127Q.UAS.Tag:HA}

(Mallik and Lakhotia, 2010)

lncRNA:Hsrω^EP3037/Scer\GAL4^GMR.PF is an enhancer of partially lethal - majority die | pupal stage phenotype of Scer\GAL4^GMR.PF, Zzzz\CAG^{127Q.UAS.Tag:HA}, nej^{F2161A.UAS.Tag:V5}

(Mallik and Lakhotia, 2010)

lncRNA:Hsrω^EP3037, Scer\GAL4^GMR.PF is an enhancer of abnormal eye color phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.PF

(Mallik and Lakhotia, 2009)

lncRNA:Hsrω^EP3037/Scer\GAL4^GMR.PF is an enhancer of abnormal eye color phenotype of Scer\GAL4^GMR.PF, Zzzz\CAG^{127Q.UAS.Tag:HA}

(Mallik and Lakhotia, 2009)

lncRNA:Hsrω^EP3037/Scer\GAL4^GMR.PF is an enhancer of abnormal neuroanatomy phenotype of Scer\GAL4^GMR.PF, Zzzz\CAG^{127Q.UAS.Tag:HA}

(Sengupta and Lakhotia, 2006)

NOT Enhancer of

Statement

Reference

lncRNA:Hsrω^EP3037/Scer\GAL4^GMR.PF is a non-enhancer of lethal | pupal stage phenotype of Scer\GAL4^GMR.PF, nej^ΔQ.UAS

(Mallik and Lakhotia, 2010)

lncRNA:Hsrω^EP3037, Scer\GAL4^GMR.PF is a non-enhancer of lethal | pupal stage phenotype of Diap1^RNAi.UAS.cLa, Scer\GAL4^GMR.PF

(Mallik and Lakhotia, 2009)

lncRNA:Hsrω^EP3037/Scer\GAL4^GMR.PF is a non-enhancer of lethal | pupal stage phenotype of Scer\GAL4^GMR.PF, egr^UAS.cIa

(Mallik and Lakhotia, 2009)

lncRNA:Hsrω^EP3037/Scer\GAL4^GMR.PF is a non-enhancer of lethal | pupal stage phenotype of Scer\GAL4^GMR.PF, Tak1^UAS.cTa

(Mallik and Lakhotia, 2009)

NOT Suppressor of

Statement

Reference

lncRNA:Hsrω^EP3037/Scer\GAL4^GMR.PF is a non-suppressor of lethal | pupal stage phenotype of Scer\GAL4^GMR.PF, nej^ΔQ.UAS

(Mallik and Lakhotia, 2010)

lncRNA:Hsrω^EP3037, Scer\GAL4^GMR.PF is a non-suppressor of lethal | pupal stage phenotype of Diap1^RNAi.UAS.cLa, Scer\GAL4^GMR.PF

(Mallik and Lakhotia, 2009)

Other

Statement

Reference

Scer\GAL4^GMR.PF, Zzzz\CAG^{127Q.UAS.Tag:HA}, lncRNA:Hsrω^EP3037, nej^RNAi.UAS.cKa has lethal | pharate adult stage phenotype

(Mallik and Lakhotia, 2010)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, lncRNA:Hsrω^EP3037, rpr^GMR.PW has eye phenotype, suppressible by lncRNA:Hsrω^RNAi.Sym.UAS/Scer\GAL4^GMR.PF

(Mallik and Lakhotia, 2009)

Scer\GAL4^GMR.PF, grim^GMR.PH, lncRNA:Hsrω^EP3037 has eye phenotype, suppressible by lncRNA:Hsrω^RNAi.Sym.UAS/Scer\GAL4^GMR.PF

(Mallik and Lakhotia, 2009)

Scer\GAL4^GMR.PF, Zzzz\CAG^{127Q.UAS.Tag:HA}, lncRNA:Hsrω^EP3037 has eye phenotype, suppressible | partially by lncRNA:Hsrω^RNAi.Sym.UAS/Scer\GAL4^GMR.PF

(Mallik and Lakhotia, 2009)

Enhancer of

Statement

Reference

lncRNA:Hsrω^EP3037/ lncRNA:Hsromega [+] is an enhancer of eye phenotype of Scer\GAL4^GMR.PU

(Mallik and Lakhotia, 2011)

lncRNA:Hsrω^EP3037, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Df(3R)Hrb87F/+, Scer\GAL4^GMR.PF, Zzzz\CAG^{127Q.UAS.Tag:HA}

(Mallik and Lakhotia, 2010)

lncRNA:Hsrω^EP3037/Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, Zzzz\CAG^{127Q.UAS.Tag:HA}, nej^{F2161A.UAS.Tag:V5}

(Mallik and Lakhotia, 2010)

lncRNA:Hsrω^EP3037/Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, nej^{F2161A.UAS.Tag:V5}

(Mallik and Lakhotia, 2010)

lncRNA:Hsrω^EP3037/Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, nej^ΔNZK.UAS

(Mallik and Lakhotia, 2010)

lncRNA:Hsrω^EP3037/Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, nej^RNAi.UAS.cKa

(Mallik and Lakhotia, 2010)

lncRNA:Hsrω^EP3037, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Diap1^RNAi.UAS.cLa, Scer\GAL4^GMR.PF

(Mallik and Lakhotia, 2009)

lncRNA:Hsrω^EP3037/Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, egr^UAS.cIa

(Mallik and Lakhotia, 2009)

lncRNA:Hsrω^EP3037/Scer\GAL4^GMR.PF is an enhancer of eye photoreceptor cell | third instar larval stage phenotype of Scer\GAL4^GMR.PF, egr^UAS.cIa

(Mallik and Lakhotia, 2009)

lncRNA:Hsrω^EP3037/Scer\GAL4^GMR.PF is an enhancer of embryonic/larval optic stalk phenotype of Scer\GAL4^GMR.PF, egr^UAS.cIa

(Mallik and Lakhotia, 2009)

lncRNA:Hsrω^EP3037/Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, Tak1^UAS.cTa

(Mallik and Lakhotia, 2009)

lncRNA:Hsrω^EP3037/Scer\GAL4^GMR.PF is an enhancer of eye phenotype of rpr^GMR.PW

(Mallik and Lakhotia, 2009)

lncRNA:Hsrω^EP3037/Scer\GAL4^GMR.PF is an enhancer of eye phenotype of grim^GMR.PH

(Mallik and Lakhotia, 2009)

lncRNA:Hsrω^EP3037, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.PF

(Mallik and Lakhotia, 2009)

lncRNA:Hsrω^EP3037, Scer\GAL4^GMR.PF is an enhancer of retina phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.PF

(Mallik and Lakhotia, 2009)

lncRNA:Hsrω^EP3037, Scer\GAL4^GMR.PF is an enhancer of rhabdomere phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^GMR.PF

(Mallik and Lakhotia, 2009)

lncRNA:Hsrω^EP3037/Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, Zzzz\CAG^{127Q.UAS.Tag:HA}

(Mallik and Lakhotia, 2009, Sengupta and Lakhotia, 2006)

NOT Enhancer of

Statement

Reference

lncRNA:Hsrω^EP3037/Scer\GAL4^GMR.PF is a non-enhancer of eye phenotype of Scer\GAL4^GMR.PF, nej^ΔBHQ.UAS

(Mallik and Lakhotia, 2010)

lncRNA:Hsrω^EP3037/Scer\GAL4^GMR.PF is a non-enhancer of eye phenotype of Scer\GAL4^GMR.PF, Traf4^EP578

(Mallik and Lakhotia, 2009)

lncRNA:Hsrω^EP3037/Scer\GAL4^GMR.PF is a non-enhancer of eye phenotype of hid^GMR.PG

(Mallik and Lakhotia, 2009)

lncRNA:Hsrω^EP3037, Scer\GAL4^GMR.PF is a non-enhancer of eye phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Mallik and Lakhotia, 2009)

lncRNA:Hsrω^EP3037, Scer\GAL4^GMR.PF is a non-enhancer of eye phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^GMR.PF

(Mallik and Lakhotia, 2009)

lncRNA:Hsrω^EP3037, Scer\GAL4^GMR.PF is a non-enhancer of eye phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^GMR.PF

(Mallik and Lakhotia, 2009)

NOT Suppressor of

Statement

Reference

lncRNA:Hsrω^EP3037/Scer\GAL4^GMR.PF is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PF, nej^ΔBHQ.UAS

(Mallik and Lakhotia, 2010)

lncRNA:Hsrω^EP3037/Scer\GAL4^GMR.PF is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PF, Traf4^EP578

(Mallik and Lakhotia, 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Coexpression of one copy of Hsrω^EP3037 in the Scer\GAL4^GMR.PF, nej^{F2161A.Scer\UAS.T:SV5\V5} background exaggerates the eye damage.

Co-expression of Hsrω^EP3037 in the Scer\GAL4^GMR.PF, nej^{dsRNA.Scer\UAS.cKa} background enhances eye degeneration with the loss of ommatidial integrity extending to most of the eye.

Co-expression of Hsrω^EP3037 enhances the eye damage seen in Scer\GAL4^GMR.PF, nej^{ΔNZK.Scer\UAS} flies.

(Mallik and Lakhotia, 2010)

Scer\GAL4^GMR.PF-mediated expression of Hsrω^EP3037 significantly enhances the rpr^GMR.PW or grim^GMR.PH eye phenotypes, but does not discernibly enhance the W^GMR.PG eye phenotype, probably because W^GMR.PG eyes are already very damaged.

Scer\GAL4^GMR.PF-mediated expression of P{Sym-UAS-Hsrω} eliminates the enhancing effect of Hsrω^EP3037 on rpr^GMR.PW or grim^GMR.PH eyes as well as reversing the level of degeneration seen in eyes expressing rpr^GMR.PW or grim^GMR.PH alone.

Co-expression of Hsrω^EP3037 leads to a slight enhancement of the eye phenotype in Scer\GAL4^GMR.PF,th^{dsRNA.Scer\UAS.cLa} flies.

Co-expression of Hsrω^EP3037 has no effect on the pupal lethality of Scer\GAL4^GMR.PF,th^{dsRNA.Scer\UAS.cLa} flies.

Coexpression of Hsrω^EP3037 enhances the Scer\GAL4^GMR.PF, egr^Scer\UAS.cIa eye phenotype, leading to severe head malformations and the formation of black lesions on the eye, though pupal lethality is not increased.

Coexpression of Hsrω^EP3037 enhances the Scer\GAL4^GMR.PF, Tak1^Scer\UAS.cTa eye phenotype, though pupal lethality is not increased.

Co-expression of Hsrω^EP3037 enhances the photoreceptor degeneration phenotype seen in Scer\GAL4^GMR.PF, egr^Scer\UAS.cIa eye discs.

Co-expression of Hsrω^EP3037 enhances the optic stalk axonal projection phenotype seen in Scer\GAL4^GMR.PF, egr^Scer\UAS.cIa eye discs.

(Mallik and Lakhotia, 2009)

Xenogenetic Interactions

Statement

Reference

The rough eye phenotype seen in the absence of any responder transgene in animals carrying two copies of Scer\GAL4^GMR.PU is enhanced by the addition of lncRNA:Hsrω^EP3037/+.

(Mallik and Lakhotia, 2011)

Expression of Hsrω^EP3037 in the Scer\GAL4^GMR.PF, Zzzz\CAG^{127Q.Scer\UAS.T:Ivir\HA1}, Df(3R)Hrb87F/+ background significantly enhances the appearence of black lesions on the eye surface, and also increases pupal mortality.

Expression of nej^{F2161A.Scer\UAS.T:SV5\V5} in the Scer\GAL4^GMR.PF, Zzzz\CAG^{127Q.Scer\UAS.T:Ivir\HA1} background results in 23% lethality at the pupal stage. Surviving flies show a reduction in eye size and a greater disruption in ommatidial arrays. Additional co-expression of Hsrω^EP3037 results in a greater reduction in eye size, near complete loss of ommatidial arrays and bristles and the appearence of black lesions on the eye surface. Co-expression of Hsrω^EP3037 also enhances pupal lethality.

(Mallik and Lakhotia, 2010)

Co-expression of Hsrω^EP3037 significantly enhances the Zzzz\CAG^{127Q.Scer\UAS.T:Ivir\HA1}, Scer\GAL4^GMR.PF eye phenotype, leading to extensive black lesions on the eye surface.

Co-expression of a single copy of P{Sym-UAS-Hsrω} eliminates the enhancing effect of Hsrω^EP3037 expression on the Zzzz\CAG^{127Q.Scer\UAS.T:Ivir\HA1}, Scer\GAL4^GMR.PF eye phenotype, and also substantially reverses the eye degeneration primarily induced by Zzzz\CAG^{127Q.Scer\UAS.T:Ivir\HA1}.

(Mallik and Lakhotia, 2009)

The neurodegeneration phenotype caused by expression of Zzzz\CAG^{127Q.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^GMR.PF is enhanced by co-expression of Hsrω^EP3037 under the control of Scer\GAL4^GMR.PF; extensive black necrotic lesions are seen in the eye.

(Sengupta and Lakhotia, 2006)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Separable from: A secondary mutation causing recessive embryonic lethality.

(Mallik and Lakhotia, 2009)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

EP3037

(Mallik and Lakhotia, 2011, Mallik and Lakhotia, 2009, Sengupta and Lakhotia, 2006)

Hsrω^EP3037

lncRNA:Hsrω^EP3037

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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