FlyBase Allele Report: Dmel\nej[ΔQ.UAS]

General Information

Symbol

Dmel\nej^ΔQ.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0175575

Feature type

allele

Associated gene

Dmel\nej

Associated Insertion(s)

Carried in Construct

P{UAS-nej.ΔQ}

Also Known As

UAS-CBP ΔQ

Key Links

Transgenic product class

inframe_deletion

(Kumar et al., 2004)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Kumar et al., 2004)

Mutagen

in vitro construct

(Kumar et al., 2004)

Progenitor genotype

Carried in construct

P{UAS-nej.ΔQ}

Cytology

Description

UASt regulatory sequences drive expression of a truncated form of nej (encodes amino acids 1-2677).

(Kumar et al., 2004)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

nej

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Alzheimer's disease

modeled by Hsap\APP^{Aβ42.UAS.cUa}

(Cutler et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

eye, with Scer\GAL4^ey.PH

(Kumar et al., 2004)

eye, with Scer\GAL4^hs.2sev

(Kumar et al., 2004)

eye disc, with Scer\GAL4^ey.PH

(Kumar et al., 2004)

eye photoreceptor cell, with Scer\GAL4^hs.2sev

(Anderson et al., 2005)

ommatidial precursor cluster, with Scer\GAL4^GMR.PF

(Kumar et al., 2004)

ommatidium, with Scer\GAL4^hs.2sev

(Anderson et al., 2005)

Detailed Description

Statement

Reference

Expression of nej^ΔQ.Scer\UAS under the control of Scer\GAL4^GMR.PU does not result in any obvious eye phenotypes in adults.

(Cutler et al., 2015)

Scer\GAL4^GMR.PF-driven expression of nej^ΔQ.Scer\UAS results in 100% lethality at the early pupal stage.

(Mallik and Lakhotia, 2010)

Expression of nej^ΔQ.Scer\UAS under the control of Scer\GAL4^hs.2sev results in loss of ommatidia within the middle of the retina. The remaining ommatidia lack variable numbers of photoreceptors.

(Anderson et al., 2005)

Animals expressing nej^ΔQ.Scer\UAS under the control of Scer\GAL4^GMR.PF die shortly after cessation of larval development. Each ommatidium in the larval eye disc contains only the R8, R2 and R5 photoreceptors, suggesting that ommatidial assembly is blocked at the recruitment of the photoreceptor R3/R4 pair. Expression of nej^ΔQ.Scer\UAS under the control of Scer\GAL4^hs.2sev results in almost complete deletion of the eye. Expression of nej^ΔQ.Scer\UAS under the control of Scer\GAL4^ey.PH blocks initiation pattern formation in the eye disc, which results in complete elimination of the eye in the adult. The severe rough eye phenotype caused by expression of nej^ΔQ.Scer\UAS under the control of Scer\GAL4^hs.2sev is not affected if the flies also carry nej³/+.

(Kumar et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, nej^ΔQ.UAS has lethal | pupal stage phenotype, non-enhanceable by lncRNA:Hsrω^EP3037/Scer\GAL4^GMR.PF

(Mallik and Lakhotia, 2010)

Scer\GAL4^GMR.PF, nej^ΔQ.UAS has lethal | pupal stage phenotype, non-enhanceable by Scer\GAL4^GMR.PF/lncRNA:Hsrω^EP93D

(Mallik and Lakhotia, 2010)

Scer\GAL4^GMR.PF, nej^ΔQ.UAS has lethal | pupal stage phenotype, non-enhanceable by lncRNA:Hsrω^RNAi.Sym.UAS/Scer\GAL4^GMR.PF

(Mallik and Lakhotia, 2010)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, nej^ΔQ.UAS has lethal | pupal stage phenotype, non-suppressible by lncRNA:Hsrω^RNAi.Sym.UAS/Scer\GAL4^GMR.PF

(Mallik and Lakhotia, 2010)

Scer\GAL4^GMR.PF, nej^ΔQ.UAS has lethal | pupal stage phenotype, non-suppressible by lncRNA:Hsrω^EP3037/Scer\GAL4^GMR.PF

(Mallik and Lakhotia, 2010)

Scer\GAL4^GMR.PF, nej^ΔQ.UAS has lethal | pupal stage phenotype, non-suppressible by Scer\GAL4^GMR.PF/lncRNA:Hsrω^EP93D

(Mallik and Lakhotia, 2010)

Suppressor of

Statement

Reference

nej^ΔQ.UAS, Scer\GAL4^GMR.PU is a suppressor of abnormal size | adult stage phenotype of Hsap\APP^{Aβ42.UAS.cUa}, Scer\GAL4^GMR.PU

(Cutler et al., 2015)

nej^ΔQ.UAS, Scer\GAL4^GMR.PU is a suppressor of increased cell death | third instar larval stage phenotype of Hsap\APP^{Aβ42.UAS.cUa}, Scer\GAL4^GMR.PU

(Cutler et al., 2015)

NOT Suppressor of

Statement

Reference

nej^ΔQ.UAS/Scer\GAL4^unspecified is a non-suppressor of visible | dominant phenotype of so^D

(Kumar et al., 2004)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^hs.2sev, nej^ΔQ.UAS has eye phenotype, enhanceable by CrebA^UAS.cRa

(Anderson et al., 2005)

Scer\GAL4^GMR.PF, nej^ΔQ.UAS has eye phenotype, enhanceable by Ubx^unspecified

(Anderson et al., 2005)

Scer\GAL4^GMR.PF, nej^ΔQ.UAS has eye phenotype, enhanceable by sog^unspecified

(Anderson et al., 2005)

Scer\GAL4^GMR.PF, nej^ΔQ.UAS has eye phenotype, enhanceable by Scr^unspecified

(Anderson et al., 2005)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, nej^ΔQ.UAS has eye phenotype, suppressible by srp^unspecified

(Anderson et al., 2005)

Scer\GAL4^GMR.PF, nej^ΔQ.UAS has eye phenotype, suppressible by Glo1^unspecified

(Anderson et al., 2005)

Scer\GAL4^GMR.PF, nej^ΔQ.UAS has eye phenotype, suppressible by RhoGAP93B^unspecified

(Anderson et al., 2005)

Scer\GAL4^GMR.PF, nej^ΔQ.UAS has eye phenotype, suppressible by Tsp42Ea^unspecified

(Anderson et al., 2005)

Scer\GAL4^GMR.PF, nej^ΔQ.UAS has eye phenotype, suppressible by cgd^unspecified

(Anderson et al., 2005)

NOT suppressed by

Statement

Reference

Scer\GAL4^hs.2sev, nej^ΔQ.UAS has eye phenotype, non-suppressible by Df(3L)H99

(Anderson et al., 2005)

Suppressor of

Statement

Reference

nej^ΔQ.UAS, Scer\GAL4^GMR.PU is a suppressor of eye phenotype of Hsap\APP^{Aβ42.UAS.cUa}, Scer\GAL4^GMR.PU

(Cutler et al., 2015)

nej^ΔQ.UAS, Scer\GAL4^GMR.PU is a suppressor of lens phenotype of Hsap\APP^{Aβ42.UAS.cUa}, Scer\GAL4^GMR.PU

(Cutler et al., 2015)

nej^ΔQ.UAS, Scer\GAL4^GMR.PU is a suppressor of eye disc | third instar larval stage phenotype of Hsap\APP^{Aβ42.UAS.cUa}, Scer\GAL4^GMR.PU

(Cutler et al., 2015)

NOT Suppressor of

Statement

Reference

nej^ΔQ.UAS/Scer\GAL4^unspecified is a non-suppressor of eye phenotype of so^D

(Kumar et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of nej^ΔQ.Scer\UAS under the control of Scer\GAL4^unspecified does not suppress the no-eye phenotype of so^D/+.

(Kumar et al., 2004)

Xenogenetic Interactions

Statement

Reference

Co-expression of nej^ΔQ.Scer\UAS partially rescues the small eye and lens fusion phenotype seen in adult flies expressing Hsap\APP^{Aβ42.Scer\UAS.cUa} under the control of Scer\GAL4^GMR.PU. Co-expression of nej^ΔQ.Scer\UAS partially suppresses the increased cell death observed in third instar eye discs of flies expressing Hsap\APP^{Aβ42.Scer\UAS.cUa} under the control of Scer\GAL4^GMR.PU.

(Cutler et al., 2015)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

nej^ΔQ.Scer\UAS

nej^ΔQ.UAS

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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