Insertion within the coding sequence.
decreased fecundity | female (with OtePK)
decreased fecundity | male | progressive (with OtePK)
decreased size | adult stage | female (with OtePK)
female sterile (with OtePK)
escort cell | adult stage (with OtePK)
fusome (with Df(2R)ED3636)
germarium (with Df(2R)ED3636)
germarium cap cell | adult stage | progressive (with OtePK)
hub cell | adult stage (with OtePK)
ovary | adult stage (with OtePK)
spectrosome (with OteEMS)
spectrosome (with OtePK)
OtePK/OteB279 mutant females display a severe reduction in the number of eggs laid per day, and males are fertile in the first 6 days of adulthood but exhibit significantly decreased fertility with age. Ovaries from OtePK/OteB279 mutant adult females contain normal numbers of terminal filament cells, but decreased numbers of cap cells and escort cells, and some mutants show loss of germline stem cells, and absence of differentiating germ cells, as compared to wild type. Cap cell number further declines with age, and the decreased cap cell number is more pronounced in mutant germaria that do not contain germ cells. In the larval ovary, OtePK/OteB279 mutants display delayed terminal filament formation. A subset of OtePK/OteB279 mutant adult males display testis phenotypes, including varying degrees of germ cell and germline stem cell loss that worsen with age, decreased cell adhesion in the niche, on average fewer (but more variability in number of) hub cells in the niche, and increasing variability in hub size with age, but no defect in somatic cyst stem cell maintenance, as compared to wild type. There is no association of germ cell loss with canonical apoptosis. Male gonads are smaller in OtePK/OteB279 mutant third instar larvae than in wild type, and the niches in mutants have reduced germline stem cell numbers compared to wild type.
Ovaries from homozygous, OteB279/Df(2R)ED3636, OteB279/OtePK and OteB279/OteEMS females have a range of mutant germarial phenotypes. These include germaria containing no germ cells, germaria containing fusomes only, germaria containing both spectrosomes and fusomes (the vast majority of this class carry a single developing cyst connected by an incompletely branched fusome) and germaria containing spectrosomes only (germaria in this class contain a higher number of spectrosome containing cells than normal). The "empty germaria" and "spectrosomes only" classes are recovered at high frequency. The phenotype is sensitive to genetic background.
Ovaries of OteB279/OtePK third instar larvae have a normal cellular organisation, with approximately 30% fewer primordial germ cells (PGCs) than age-matched control ovaries. The mutant PGCs all contain spectrosomes with no evidence of branching. Niches are formed in the mutant gonads at the pupal stage and occupied by germ cells, as occurs in wild type. However, the mutant pupal gonads do not contain differentiated egg chambers (egg chambers are established in wild type pupal gonads).
Homozygous germline stem cell clones are lost more quickly from ovaries compared to control clones.
Nemp[+]/NempRR1, OteB279 has decreased cell number | female | adult stage phenotype
Nemp[+]/NempRR1, OtePK/OteB279 has decreased cell number | female | adult stage phenotype
OtePK/OteB279, bocksΔ10/bocksΔ66 has abnormal mitotic cell cycle phenotype
OtePK/OteB279, bocksΔ10/bocksΔ66 has some die during P-stage phenotype
OtePK/OteB279, bocksΔ10/bocksΔ66 has majority die during larval stage phenotype
OtePK/OteB279, bocksΔ10/bocksΔ66 has decreased occurrence of cell division | larval stage phenotype
MAN1Δ26/MAN1Δ81, OtePK/OteB279 has some die during P-stage phenotype
MAN1Δ26/MAN1Δ81, OtePK/OteB279 has some die during larval stage phenotype
Nemp[+]/NempRR1, OteB279 has ovary | adult stage phenotype
Nemp[+]/NempRR1, OteB279 has female germline cell | adult stage | decreased number phenotype
Nemp[+]/NempRR1, OtePK/OteB279 has ovary | adult stage phenotype
Nemp[+]/NempRR1, OtePK/OteB279 has female germline cell | adult stage | decreased number phenotype
OtePK/OteB279, bocksΔ10/bocksΔ66 has imaginal disc phenotype
OtePK/OteB279, bocksΔ10/bocksΔ66 has embryonic/larval brain | larval stage phenotype
OtePK/OteB279 is partially rescued by Otenanos.PB
OtePK/OteB279 is not rescued by OteUASp.cBa/Scer\GAL4en.PU
OtePK/OteB279 is not rescued by OteUASp.cBa/Scer\GAL4bab1-Gal4
OtePK/OteB279 is not rescued by OteUASp.cBa/Scer\GAL4C587
Expression of Otenos.PB restores fertility, rescues the germline stem cell maintenance, differentiation germ cell number, somatic cell number and cell adhesion phenotypes of both OtePK/OteB279 mutant males and females, but does not fully restore hub size in mutant males.
Expression of OteScer\UAS.P\T.cBa under the control of Scer\GAL4en.PU, Scer\GAL4bab1-Gal4, or Scer\GAL4C587 fails to rescue the sterility, loss of germline stem cells, lack of differentiating germ cells, or loss of cap cells observed in OtePK/OteB279 mutant females.